PMID- 31975318
OWN - NLM
STAT- MEDLINE
DCOM- 20201008
LR  - 20201008
IS  - 1567-2387 (Electronic)
IS  - 1567-2379 (Linking)
VI  - 51
IP  - 1
DP  - 2020 Feb
TI  - K31 as a novel marker for clear secretory cells in human eccrine sweat glands.
PG  - 47-53
LID - 10.1007/s10735-020-09855-y [doi]
AB  - K31 was previously considered as one of the hair keratins. During a study on 
      differential markers between hair follicles and eccrine sweat glands, we observed 
      that K31 was expressed in eccrine sweat gland cells in a scattered pattern, 
      similar to the distribution of dark or clear secretory cells. To investigate the 
      precise cell localization of K31 in human eccrine sweat glands and find new 
      marker for eccrine sweat gland cells, human skin samples were fixed, paraffined 
      and sectioned. The serial sections were stained for K31, dark secretory cell 
      marker gross cystic disease fluid protein 15 (GCDFP15) and clear secretory cell 
      marker carbonic anhydrase II (CAII). The exact cell localization of K31 was 
      detected by double immunofluorescence staining of K31 and a serial of 
      cell-specific markers, and further by dual stain using a combination of periodic 
      acid-Schiff (PAS) and immunofluorescence for K31 and GCDFP15. The expression 
      pattern of K31-positive cells was similar to that of CAII-positive cells but was 
      different from that of GCDFP15-positive staining in serial sections. Double 
      immunofluorescent staining showed that K31-positive cells co-expressed K7 and 
      CAII, but not S100P, alpha-SMA or GCDFP15. Dual stain by combined PAS and 
      immunofluorescence showed that K31-positive cells are negative for PAS staining. 
      We conclude that K31 is a previously unreported eccrine clear cell marker that 
      allows for distinction between clear and dark secretory cells, as well as between 
      secretory coils and ducts of eccrine sweat glands in human eccrine sweat glands.
FAU - Du, Lijie
AU  - Du L
AD  - Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei 
      University of Medicine, 32 South Renmin Road, Shiyan, 442000, Hubei, China.
FAU - Zhang, Xiang
AU  - Zhang X
AD  - Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei 
      University of Medicine, 32 South Renmin Road, Shiyan, 442000, Hubei, China.
FAU - Chen, Liyun
AU  - Chen L
AD  - Department of Plastic Surgery and Burn Center, Second Affiliated Hospital, 
      Shantou University Medical College, North Dongxia Road, Shantou, 515041, 
      Guangdong, China.
FAU - Zhang, Lei
AU  - Zhang L
AD  - Mental Health Center, Taihe Hospital, Hubei University of Medicine, 32 South 
      Renmin Road, Shiyan, 442000, Hubei, China.
FAU - Li, Haihong
AU  - Li H
AD  - Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei 
      University of Medicine, 32 South Renmin Road, Shiyan, 442000, Hubei, China. 
      lihaihong1051@126.com.
LA  - eng
GR  - 81772102/National Natural Science Foundation of China/
GR  - 81471882/National Natural Science Foundation of China/
PT  - Clinical Trial
PT  - Journal Article
DEP - 20200123
PL  - Netherlands
TA  - J Mol Histol
JT  - Journal of molecular histology
JID - 101193653
RN  - 0 (Antigens, Differentiation)
RN  - 0 (KRT31 protein, human)
RN  - 0 (Keratins, Hair-Specific)
RN  - 0 (Keratins, Type I)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (PIP protein, human)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Antigens, Differentiation/*biosynthesis
MH  - Child
MH  - Eccrine Glands/cytology/*metabolism
MH  - Female
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Keratins, Hair-Specific/*biosynthesis
MH  - Keratins, Type I/*biosynthesis
MH  - Male
MH  - Membrane Transport Proteins/biosynthesis
MH  - Middle Aged
OTO - NOTNLM
OT  - Clear secretory cells
OT  - Eccrine sweat glands
OT  - K31
OT  - Marker
EDAT- 2020/01/25 06:00
MHDA- 2020/10/09 06:00
CRDT- 2020/01/25 06:00
PHST- 2019/10/22 00:00 [received]
PHST- 2020/01/20 00:00 [accepted]
PHST- 2020/01/25 06:00 [pubmed]
PHST- 2020/10/09 06:00 [medline]
PHST- 2020/01/25 06:00 [entrez]
AID - 10.1007/s10735-020-09855-y [pii]
AID - 10.1007/s10735-020-09855-y [doi]
PST - ppublish
SO  - J Mol Histol. 2020 Feb;51(1):47-53. doi: 10.1007/s10735-020-09855-y. Epub 2020 
      Jan 23.