PMID- 31978319
OWN - NLM
STAT- MEDLINE
DCOM- 20210202
LR  - 20231213
IS  - 1557-8976 (Electronic)
IS  - 0882-8245 (Linking)
VI  - 33
IP  - 1
DP  - 2020 Jan/Feb
TI  - Mechanisms of mTOR and Autophagy in Human Endothelial Cell Infected with Dengue 
      Virus-2.
PG  - 61-70
LID - 10.1089/vim.2019.0009 [doi]
AB  - The mechanistic mammalian target of rapamycin (mTOR) plays a crucial role in 
      response to many major cellular processes, including cellular metabolism, 
      proliferation, and autophagy. Both mTOR and autophagy are suggested to be 
      involved in the viral infection. However, little is known about the role of mTOR 
      and autophagy in human endothelial cell infected with dengue virus-2 (DENV-2), 
      this study is to investigate the role of mTOR and autophagy in human umbilical 
      vein endothelial cells (HUVECs) infected with DENV-2 and related regulatory 
      mechanisms. HUVECs were cultured in epithelial cell medium. A series of 
      experiments involving immunohistochemistry, TCID(50) method, real-time PCR, 
      western blot, and laser confocal were performed in this study. The cell line was 
      identified as HUVEC by the expression of cell factor VIII. The expression level 
      of DENV-2 mRNA increased and showed an upward trend. Compared with the control 
      group, the fluorescence of autophagy-labeled protein LC3B and lysosome-labeled 
      protein lysosome-associated membrane protein 1 (LAMP1) in the cytoplasm of HUVEC 
      induced by rapamycin was observed, and intensity was significantly enhanced under 
      confocal laser scanning microscope, after fluorescence synthesis, the 
      fluorescence of autophagy-labeled protein LC3B and lysosome-labeled protein LAMP1 
      overlaps were reduced. The intensity of fluorescence of autophagy-labeled protein 
      LC3B and lysosome-labeled protein LAMP1 increased in 1 x 10(4) TCID(50) DENV-2 
      infection group, after fluorescence synthesis, fluorescence of autophagy-labeled 
      protein LC3B, lysosome-labeled protein LAMP1, and DEN2 NS1 overlapped. Compared 
      with the control group, the phosphorylation level of mTOR, Atg13, and p-ULK1 in 
      DENV-2-infected group or Rapa treatment group decreased significantly (p < 0.05), 
      and the level of LC3-II increased significantly (p < 0.05). These results suggest 
      that DENV-2 induces autophagy in HUVECs through mTOR signaling molecule.
FAU - Kong, Weiying
AU  - Kong W
AD  - Department of Immunology, Guizhou Medical University, Guiyang, China.
AD  - Center for Clinical Laboratories, The Affiliated Hospital of Guizhou Medical 
      University, Guiyang, China.
FAU - Mao, Jiaxuan
AU  - Mao J
AD  - Department of Immunology, Guizhou Medical University, Guiyang, China.
FAU - Yang, Yang
AU  - Yang Y
AD  - Department of Immunology, Guizhou Medical University, Guiyang, China.
FAU - Yuan, Jing
AU  - Yuan J
AD  - Department of Immunology, Guizhou Medical University, Guiyang, China.
FAU - Chen, Junhao
AU  - Chen J
AD  - Department of Immunology, Guizhou Medical University, Guiyang, China.
FAU - Luo, Yu
AU  - Luo Y
AD  - Department of Immunology, Guizhou Medical University, Guiyang, China.
FAU - Lai, Tao
AU  - Lai T
AD  - Department of Immunology, Guizhou Medical University, Guiyang, China.
FAU - Zuo, Li
AU  - Zuo L
AD  - Department of Immunology, Guizhou Medical University, Guiyang, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Viral Immunol
JT  - Viral immunology
JID - 8801552
RN  - 0 (LAMP1 protein, human)
RN  - 0 (Lysosomal Membrane Proteins)
RN  - 0 (MAP1LC3B protein, human)
RN  - 0 (Microtubule-Associated Proteins)
RN  - 0 (NS1 protein, Dengue virus type 2)
RN  - 0 (Viral Nonstructural Proteins)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - *Autophagy
MH  - Cell Line
MH  - Dengue Virus/genetics/*pathogenicity
MH  - Fluorescence
MH  - Human Umbilical Vein Endothelial Cells/drug effects/pathology/*virology
MH  - Humans
MH  - Lysosomal Membrane Proteins/genetics
MH  - Microtubule-Associated Proteins/genetics
MH  - Phosphorylation
MH  - Signal Transduction
MH  - Sirolimus/pharmacology
MH  - TOR Serine-Threonine Kinases/*genetics
MH  - Viral Nonstructural Proteins/genetics
OTO - NOTNLM
OT  - DENV-2
OT  - HUVECs
OT  - LC3B
OT  - autophagy
OT  - mTOR
EDAT- 2020/01/25 06:00
MHDA- 2021/02/03 06:00
CRDT- 2020/01/25 06:00
PHST- 2020/01/25 06:00 [entrez]
PHST- 2020/01/25 06:00 [pubmed]
PHST- 2021/02/03 06:00 [medline]
AID - 10.1089/vim.2019.0009 [doi]
PST - ppublish
SO  - Viral Immunol. 2020 Jan/Feb;33(1):61-70. doi: 10.1089/vim.2019.0009.