PMID- 31979387
OWN - NLM
STAT- MEDLINE
DCOM- 20201127
LR  - 20231113
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 25
IP  - 3
DP  - 2020 Jan 23
TI  - A New Method for Determination of Thymol and Carvacrol in Thymi herba by 
      Ultraperformance Convergence Chromatography (UPC(2)).
LID - 10.3390/molecules25030502 [doi]
LID - 502
AB  - Ultraperformance convergence chromatography is an environmentally friendly 
      analytical technique for dramatically reducing the use of organic solvents 
      compared to conventional chromatographic methods. In this study, a rapid and 
      sensitive ultraperformance convergence chromatography method was firstly 
      established for quantification of thymol and carvacrol, two positional isomers of 
      a major bioactive in the volatile oil of Thymi herba, the dried leaves and 
      flowers of Thymus mongolicus or Thymus przewalskii, known in China as "Dijiao." 
      Using a Trefoil(TM) CEL1 column, thymol and carvacrol were separated in less than 
      2.5 min and resolution was enhanced. The method was validated with respect to 
      precision, accuracy, and linearity according to the National Medical Products 
      Administration guidelines. The optimized method exhibited good linear correlation 
      (r = 0.9998-0.9999), excellent precision (relative standard deviations (RSDs) < 
      1.50%), and acceptable recoveries (87.29-102.89%). The limits of detection for 
      thymol and carvacrol were 1.31 and 1.57 ng/L, respectively, while their 
      corresponding limits of quantification were 2.63 and 3.14 ng/L. Finally, the 
      quantities of the two compounds present in 16 T. mongolicus and four T. 
      przewalskii samples were successfully evaluated by employing the developed 
      method. It is hoped that the results of this study will serve as a guideline for 
      the quality control of Thymi herba.
FAU - Chang, Xiaoqiang
AU  - Chang X
AD  - Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 
      Beijing 100700, China.
AD  - Artemisinin Research Center, China Academy of Chinese Medical Sciences, Beijing 
      100700, China.
FAU - Sun, Peng
AU  - Sun P
AD  - Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 
      Beijing 100700, China.
AD  - Artemisinin Research Center, China Academy of Chinese Medical Sciences, Beijing 
      100700, China.
FAU - Ma, Yue
AU  - Ma Y
AD  - Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 
      Beijing 100700, China.
AD  - Artemisinin Research Center, China Academy of Chinese Medical Sciences, Beijing 
      100700, China.
FAU - Han, Dongchen
AU  - Han D
AD  - Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 
      Beijing 100700, China.
AD  - Artemisinin Research Center, China Academy of Chinese Medical Sciences, Beijing 
      100700, China.
FAU - Zhao, Yifan
AU  - Zhao Y
AD  - Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 
      Beijing 100700, China.
AD  - Artemisinin Research Center, China Academy of Chinese Medical Sciences, Beijing 
      100700, China.
FAU - Bai, Yue
AU  - Bai Y
AD  - Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 
      Beijing 100700, China.
AD  - Artemisinin Research Center, China Academy of Chinese Medical Sciences, Beijing 
      100700, China.
FAU - Zhang, Dong
AU  - Zhang D
AD  - Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 
      Beijing 100700, China.
AD  - Artemisinin Research Center, China Academy of Chinese Medical Sciences, Beijing 
      100700, China.
FAU - Yang, Lan
AU  - Yang L
AD  - Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 
      Beijing 100700, China.
AD  - Artemisinin Research Center, China Academy of Chinese Medical Sciences, Beijing 
      100700, China.
LA  - eng
GR  - 2060302/The ability establishment of sustainable use for valuable Chinese 
      medicine resources/
PT  - Journal Article
DEP - 20200123
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Cymenes)
RN  - 0 (Oils, Volatile)
RN  - 3J50XA376E (Thymol)
RN  - 9B1J4V995Q (carvacrol)
SB  - IM
MH  - China
MH  - Chromatography/instrumentation/*methods
MH  - Cymenes/*analysis/chemistry/isolation & purification
MH  - Flowers/chemistry
MH  - Isomerism
MH  - Oils, Volatile/chemistry
MH  - Plant Leaves/chemistry
MH  - Thymol/*analysis/chemistry/isolation & purification
MH  - Thymus Plant/*chemistry
PMC - PMC7037148
OTO - NOTNLM
OT  - Thymus mongolicus
OT  - Thymus przewalskii
OT  - carvacrol
OT  - thymol
OT  - ultraperformance convergence chromatography
COIS- We declare that there is no conflict of interest with this work.
EDAT- 2020/01/26 06:00
MHDA- 2020/11/28 06:00
PMCR- 2020/01/23
CRDT- 2020/01/26 06:00
PHST- 2019/12/19 00:00 [received]
PHST- 2020/01/15 00:00 [revised]
PHST- 2020/01/21 00:00 [accepted]
PHST- 2020/01/26 06:00 [entrez]
PHST- 2020/01/26 06:00 [pubmed]
PHST- 2020/11/28 06:00 [medline]
PHST- 2020/01/23 00:00 [pmc-release]
AID - molecules25030502 [pii]
AID - molecules-25-00502 [pii]
AID - 10.3390/molecules25030502 [doi]
PST - epublish
SO  - Molecules. 2020 Jan 23;25(3):502. doi: 10.3390/molecules25030502.