PMID- 31980137
OWN - NLM
STAT- MEDLINE
DCOM- 20210111
LR  - 20210111
IS  - 1618-0372 (Electronic)
IS  - 0065-1281 (Linking)
VI  - 122
IP  - 3
DP  - 2020 Apr
TI  - miRNA-183 approximately 96 approximately 182 regulates melanogenesis, cell proliferation and migration in B16 
      cells.
PG  - 151508
LID - S0065-1281(20)30007-6 [pii]
LID - 10.1016/j.acthis.2020.151508 [doi]
AB  - Melanoma is a highly invasive malignant skin tumor having high metastatic rate 
      and poor prognosis. The biology of melanoma is controled by miRNAs. The miRNA-183 
      cluster, which is composed of miRNA-183 approximately 96 approximately 182 genes, plays an important roles in 
      tumor development. In order to investigate the role and action of miRNA-183 
      cluster in B16 cells, we overexpressed and knocked down miRNA-183 cluster in B16 
      cells. Using bioinformatics analysis, we predicted that the key framscript factor 
      of melangenic genes. Microphthalmia-associated transcription factor (MITF) is one 
      of the targets of miRNA-183 cluster. The results of Luciferase activity assays 
      confirmed that MITF was targeted by miRNA-183 cluster. Overexpression and 
      knockdown of miRNA-183 cluster in B16 cells resulted in down and up regulation of 
      MITF expression, respectively at both mRNA and protein levels. Furthmore, 
      overexpression and knockdown of the miRNA-183 cluster in B16 cells decreased and 
      increased the expression of mRNA and protein of melangenic genes tyrosinase 
      (TYR), and tyrosinase-related protein 1 (TYRP1), dopachrome-tautomerase (DCT), as 
      well as the production of melanins and eumelanin production, respectively. On the 
      proliferation and migration pathway, overexpression and knockdown of miRNA-183 
      cluster increased and decreased, respectively the expression of mRNA and protein 
      of mitogen-activated protein kinase 1 (MEK1), extracellular regulated protein 
      kinases1/2 (ERK1/2) and cAMP-responsive-element binding protein (CREB). These 
      results indicated that miRNA-183 cluster regulated melanogenesis in B16 cells as 
      well as cell proliferation and migration by directly targeting MITF through 
      migration pathway.
CI  - Copyright (c) 2020 Elsevier GmbH. All rights reserved.
FAU - Du, Bin
AU  - Du B
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China.
FAU - Liu, Xuexian
AU  - Liu X
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China.
FAU - Khan, Ajab
AU  - Khan A
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China.
FAU - Wan, Shuangxiu
AU  - Wan S
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China.
FAU - Guo, Xiang
AU  - Guo X
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China.
FAU - Xue, Jixuan
AU  - Xue J
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China.
FAU - Fan, Ruiwen
AU  - Fan R
AD  - College of Animal Science and Veterinary Medicine, Shanxi Agricultural 
      University, Taigu, 030801, China. Electronic address: ruiwenfan@163.com.
LA  - eng
PT  - Journal Article
DEP - 20200121
PL  - Germany
TA  - Acta Histochem
JT  - Acta histochemica
JID - 0370320
RN  - 0 (MITF protein, human)
RN  - 0 (Melanins)
RN  - 0 (MicroRNAs)
RN  - 0 (Microphthalmia-Associated Transcription Factor)
RN  - 12627-86-0 (eumelanin)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.14.18.- (tyrosinase-related protein-1)
RN  - EC 1.14.18.1 (Monophenol Monooxygenase)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 1)
RN  - EC 2.7.12.2 (MAP2K1 protein, human)
SB  - IM
MH  - Cell Movement/*genetics
MH  - *Cell Proliferation
MH  - Computational Biology
MH  - Gene Expression Regulation/*genetics
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - MAP Kinase Kinase 1/biosynthesis/genetics
MH  - MAP Kinase Signaling System/genetics
MH  - Melanins/*biosynthesis/*genetics
MH  - Melanocytes/metabolism
MH  - Melanoma, Experimental/*genetics
MH  - MicroRNAs/*genetics
MH  - Microphthalmia-Associated Transcription Factor/biosynthesis/genetics
MH  - Monophenol Monooxygenase/metabolism
MH  - Multigene Family
MH  - Oxidoreductases
OTO - NOTNLM
OT  - B16 cell
OT  - MAPK
OT  - MITF
OT  - Melanogenesis
OT  - Migration
OT  - Proliferation
OT  - microRNA-183 cluster
COIS- Declaration of Competing Interest The authors declare no conflict of interest.
EDAT- 2020/01/26 06:00
MHDA- 2021/01/12 06:00
CRDT- 2020/01/26 06:00
PHST- 2019/10/25 00:00 [received]
PHST- 2020/01/06 00:00 [revised]
PHST- 2020/01/13 00:00 [accepted]
PHST- 2020/01/26 06:00 [pubmed]
PHST- 2021/01/12 06:00 [medline]
PHST- 2020/01/26 06:00 [entrez]
AID - S0065-1281(20)30007-6 [pii]
AID - 10.1016/j.acthis.2020.151508 [doi]
PST - ppublish
SO  - Acta Histochem. 2020 Apr;122(3):151508. doi: 10.1016/j.acthis.2020.151508. Epub 
      2020 Jan 21.