PMID- 31981817
OWN - NLM
STAT- MEDLINE
DCOM- 20210520
LR  - 20210520
IS  - 1872-7603 (Electronic)
IS  - 0165-0378 (Linking)
VI  - 137
DP  - 2020 Feb
TI  - Effect of seminal plasma on dendritic cell differentiation in vitro depends on 
      the serum source in the culture medium.
PG  - 103076
LID - S0165-0378(19)30438-3 [pii]
LID - 10.1016/j.jri.2019.103076 [doi]
AB  - Dendritic cells (DCs) are key in shaping immune responses and are recruited to 
      the human cervix after coitus by seminal plasma (SP). SP has been shown to skew 
      the differentiation of monocyte-derived DCs towards an anti-inflammatory profile 
      when cultured in medium containing fetal calf serum (FCS). Here, we confirmed 
      that SP skewed DCs cultured in fetal bovine serum (FBS) towards a tolerogenic 
      profile. To create a setting more similar to the in vivo situations in humans, we 
      tested the immune regulatory effect of SP on DCs in cell cultures containing 
      human serum (HS). SP-DCs cultured in HS did show increased CD14 and decreased 
      CD1a, indicating an inhibited maturation phenotype. Gene expression of TGF-beta and 
      IL-10 and IL-10 protein expression were elevated in LPS-activated SP-DCs, whereas 
      IL-12p70 protein levels were decreased compared to LPS-activated control DCs. In 
      contrast to FBS culture conditions, in the presence of HS co-cultures of SP-DCs 
      with allogeneic peripheral blood mononuclear cells (PBMCs) did not result in 
      decreased T cell proliferation and inflammatory cytokine production. Thus, under 
      HS culture conditions SP can skew the differentiation of monocyte-derived DCs 
      phenotypically towards alternatively activated DCs, but this immune regulatory 
      phenotype is functionally less pronounced compared to SP-treated DCs cultured in 
      FBS containing medium. These findings highlight the importance of the source of 
      the serum that is used in SP treated cell cultures in vitro.
CI  - Copyright (c) 2019. Published by Elsevier B.V.
FAU - Craenmehr, M H C
AU  - Craenmehr MHC
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, the Netherlands. Electronic address: M.H.C.craenmehr@lumc.nl.
FAU - van der Keur, C
AU  - van der Keur C
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, the Netherlands. Electronic address: C.van_der_Keur@lumc.nl.
FAU - Anholts, J D H
AU  - Anholts JDH
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, the Netherlands. Electronic address: J.D.H.Anholts@lumc.nl.
FAU - Kapsenberg, J M
AU  - Kapsenberg JM
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, the Netherlands. Electronic address: J.M.Kapsenberg@lumc.nl.
FAU - van der Westerlaken, L A
AU  - van der Westerlaken LA
AD  - Department of Gynaecology, Leiden University Medical Center, Leiden, the 
      Netherlands. Electronic address: L.A.J.van_der_Westerlaken@lumc.nl.
FAU - van Kooten, C
AU  - van Kooten C
AD  - Department of Nephrology, Leiden University Medical Center, Leiden, the 
      Netherlands. Electronic address: C.van_Kooten@lumc.nl.
FAU - Claas, F H J
AU  - Claas FHJ
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, the Netherlands. Electronic address: F.H.J.Claas@lumc.nl.
FAU - Heidt, S
AU  - Heidt S
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, the Netherlands. Electronic address: S.Heidt@lumc.nl.
FAU - Eikmans, M
AU  - Eikmans M
AD  - Department of Immunohematology and Blood Transfusion, Leiden University Medical 
      Center, Leiden, the Netherlands. Electronic address: M.Eikmans@lumc.nl.
LA  - eng
PT  - Journal Article
DEP - 20191224
PL  - Ireland
TA  - J Reprod Immunol
JT  - Journal of reproductive immunology
JID - 8001906
RN  - 0 (Culture Media)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - ZIF514RVZR (Serum Albumin, Human)
SB  - IM
MH  - Cell Culture Techniques
MH  - Cell Differentiation/*immunology
MH  - Cells, Cultured
MH  - Culture Media/*metabolism
MH  - Dendritic Cells/*physiology
MH  - Humans
MH  - Immune Tolerance
MH  - Leukocytes, Mononuclear
MH  - Male
MH  - Primary Cell Culture/*methods
MH  - Semen/*immunology
MH  - Serum Albumin, Bovine/metabolism
MH  - Serum Albumin, Human/metabolism
OTO - NOTNLM
OT  - Dendritic cell
OT  - Fetal bovine serum
OT  - Fetal calf serum
OT  - Human serum
OT  - Seminal plasma
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/01/26 06:00
MHDA- 2021/05/21 06:00
CRDT- 2020/01/26 06:00
PHST- 2019/09/02 00:00 [received]
PHST- 2019/12/10 00:00 [revised]
PHST- 2019/12/20 00:00 [accepted]
PHST- 2020/01/26 06:00 [pubmed]
PHST- 2021/05/21 06:00 [medline]
PHST- 2020/01/26 06:00 [entrez]
AID - S0165-0378(19)30438-3 [pii]
AID - 10.1016/j.jri.2019.103076 [doi]
PST - ppublish
SO  - J Reprod Immunol. 2020 Feb;137:103076. doi: 10.1016/j.jri.2019.103076. Epub 2019 
      Dec 24.