PMID- 31983129
OWN - NLM
STAT- MEDLINE
DCOM- 20200619
LR  - 20200619
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 24
IP  - 6
DP  - 2019 Nov-Dec
TI  - Over-expression of TM4SF1 improves cell metastasis and growth by activating 
      ERK1/2 signaling pathway in human prostate cancer.
PG  - 2531-2538
AB  - PURPOSE: To explore the effects of Transmembrane-4-L-six-family-1 (TM4SF1) in 
      prostate cancer (PCa), and the related underlying mechanisms. METHODS: PCa 
      tissues were obtained from 78 patients. PCa cell lines DU145 and RWPE-2 were 
      purchased from American Type Culture Collection (ATCC). Quantitative real-time 
      polymerase chain reaction (qRT-PCR) and Western blot were conducted to analyze 
      the expression of TM4SF1 in PCa tissues and DU145 cells. Plasmid containing 
      over-expressed TM4SF1 was achieved by plasmid transfection. Transwell assay and 
      wound-healing assay were designed to examine the invasion and migration of DU145 
      cells, whereas colony formation assay and 5-Ethynyl-2'- deoxyuridine (EdU) 
      staining assay were performed to study the proliferation ability of DU145 cells. 
      RESULTS: TM4SF1 was found over-expressed in PCa tissues and DU145 cells. 
      Over-expression of TM4SF1 significantly activated the extracellular regulated 
      protein kinases (ERK)1/2 signaling pathway, increased the epithelial-mesenchymal 
      transition (EMT) expression, and enhanced the invasion, migration and 
      proliferation of DU145 cells. Further studies revealed that suppression of ERK1/2 
      signaling pathway nearly resisted the positive effects on DU145 cells induced by 
      TM4SF1 over-expression. CONCLUSIONS: The present study demonstrated that TM4SF1 
      enhanced the invasion, migration and proliferation of DU145 cells by activating 
      ERK1/2 signaling pathway.
FAU - Chen, Junyi
AU  - Chen J
AD  - Department of Urology, the Second Affiliated Hospital of Fujian Medical 
      University, Quanzhou, China.
FAU - Wang, Fubo
AU  - Wang F
FAU - Xu, Huan
AU  - Xu H
FAU - Chen, Dong
AU  - Chen D
FAU - Liu, Weihui
AU  - Liu W
FAU - Wang, Jialiang
AU  - Wang J
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Antigens, Surface)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Neoplasm Proteins)
RN  - 147016-68-0 (TM4SF1 protein, human)
RN  - EC 2.7.11.24 (MAPK1 protein, human)
RN  - EC 2.7.11.24 (MAPK3 protein, human)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 1)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Antigens, Surface/genetics/*metabolism
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Cell Movement
MH  - *Cell Proliferation
MH  - Epithelial-Mesenchymal Transition
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - Mitogen-Activated Protein Kinase 1/genetics/*metabolism
MH  - Mitogen-Activated Protein Kinase 3/genetics/*metabolism
MH  - Neoplasm Metastasis
MH  - Neoplasm Proteins/genetics/*metabolism
MH  - Prognosis
MH  - Prostatic Neoplasms/genetics/metabolism/*secondary
MH  - Tumor Cells, Cultured
MH  - Wound Healing
EDAT- 2020/01/27 06:00
MHDA- 2020/06/20 06:00
CRDT- 2020/01/27 06:00
PHST- 2020/01/27 06:00 [entrez]
PHST- 2020/01/27 06:00 [pubmed]
PHST- 2020/06/20 06:00 [medline]
PST - ppublish
SO  - J BUON. 2019 Nov-Dec;24(6):2531-2538.