PMID- 31983538
OWN - NLM
STAT- MEDLINE
DCOM- 20200714
LR  - 20240212
IS  - 1097-4199 (Electronic)
IS  - 0896-6273 (Print)
IS  - 0896-6273 (Linking)
VI  - 105
IP  - 6
DP  - 2020 Mar 18
TI  - Human Herpesvirus 6 Detection in Alzheimer's Disease Cases and Controls across 
      Multiple Cohorts.
PG  - 1027-1035.e2
LID - S0896-6273(19)31097-9 [pii]
LID - 10.1016/j.neuron.2019.12.031 [doi]
AB  - The interplay between viral infection and Alzheimer's disease (AD) has long been 
      an area of interest, but proving causality has been elusive. Several recent 
      studies have renewed the debate concerning the role of herpesviruses, and human 
      herpesvirus 6 (HHV-6) in particular, in AD. We screened for HHV-6 detection 
      across three independent AD brain repositories using (1) RNA sequencing (RNA-seq) 
      datasets and (2) DNA samples extracted from AD and non-AD control brains. The 
      RNA-seq data were screened for pathogens against taxon references from over 
      25,000 microbes, including 118 human viruses, whereas DNA samples were probed for 
      PCR reactivity to HHV-6A and HHV-6B. HHV-6 demonstrated little specificity to AD 
      brains over controls by either method, whereas other viruses, such as 
      Epstein-Barr virus (EBV) and cytomegalovirus (CMV), were detected at comparable 
      levels. These direct methods of viral detection do not suggest an association 
      between HHV-6 and AD.
CI  - Published by Elsevier Inc.
FAU - Allnutt, Mary Alice
AU  - Allnutt MA
AD  - Viral Immunology Section, National Institute of Neurological Disorders and 
      Stroke, National Institutes of Health, Bethesda, MD 20814, USA.
FAU - Johnson, Kory
AU  - Johnson K
AD  - Bioinformatics Section, Information Technology & Bioinformatics Program, Division 
      of Intramural Research (DIR), National Institute of Neurological Disorders and 
      Stroke/National Institute of Health, Bethesda, MD 20814, USA.
FAU - Bennett, David A
AU  - Bennett DA
AD  - Alzheimer Disease Center, RUSH University, Chicago, IL 60612, USA.
FAU - Connor, Sarah M
AU  - Connor SM
AD  - Center for Translational & Computational Neuroimmunology, Department of 
      Neurology, Columbia University Medical Center, New York, NY 10032, USA.
FAU - Troncoso, Juan C
AU  - Troncoso JC
AD  - Department of Pathology (Neuropathology), Johns Hopkins University Medical 
      Center, Baltimore, MD 21205, USA.
FAU - Pletnikova, Olga
AU  - Pletnikova O
AD  - Department of Pathology (Neuropathology), Johns Hopkins University Medical 
      Center, Baltimore, MD 21205, USA.
FAU - Albert, Marilyn S
AU  - Albert MS
AD  - Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, 
      MD 21205, USA.
FAU - Resnick, Susan M
AU  - Resnick SM
AD  - Laboratory of Behavioral Neuroscience, National Institute on Aging, National 
      Institutes of Health, Baltimore, MD 21224, USA.
FAU - Scholz, Sonja W
AU  - Scholz SW
AD  - Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, 
      MD 21205, USA; Neurodegenerative Diseases Research Unit, National Institute of 
      Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 
      20814, USA.
FAU - De Jager, Philip L
AU  - De Jager PL
AD  - Center for Translational & Computational Neuroimmunology, Department of 
      Neurology, Columbia University Medical Center, New York, NY 10032, USA.
FAU - Jacobson, Steven
AU  - Jacobson S
AD  - Viral Immunology Section, National Institute of Neurological Disorders and 
      Stroke, National Institutes of Health, Bethesda, MD 20814, USA. Electronic 
      address: jacobsons@ninds.nih.gov.
LA  - eng
GR  - U01 AG046152/AG/NIA NIH HHS/United States
GR  - P30 AG066507/AG/NIA NIH HHS/United States
GR  - P50 AG005146/AG/NIA NIH HHS/United States
GR  - R01 AG030146/AG/NIA NIH HHS/United States
GR  - R01 AG017917/AG/NIA NIH HHS/United States
GR  - P30 AG010161/AG/NIA NIH HHS/United States
GR  - ZIA NS003154/ImNIH/Intramural NIH HHS/United States
GR  - Z99 NS999999/ImNIH/Intramural NIH HHS/United States
GR  - U01 AG061356/AG/NIA NIH HHS/United States
GR  - U01 AG032984/AG/NIA NIH HHS/United States
GR  - R01 AG036836/AG/NIA NIH HHS/United States
GR  - R01 AG015819/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20200123
PL  - United States
TA  - Neuron
JT  - Neuron
JID - 8809320
SB  - IM
MH  - Alzheimer Disease/*virology
MH  - Brain/*virology
MH  - Case-Control Studies
MH  - Cohort Studies
MH  - Female
MH  - Herpesvirus 6, Human/genetics/*isolation & purification
MH  - Humans
MH  - Male
MH  - Sequence Analysis, DNA/statistics & numerical data
MH  - Sequence Analysis, RNA/statistics & numerical data
PMC - PMC7182308
MID - NIHMS1569250
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - herpesvirus
OT  - human herpesvirus 6
COIS- Declaration of Interests The authors declare no competing interests.
EDAT- 2020/01/28 06:00
MHDA- 2020/07/15 06:00
PMCR- 2021/03/18
CRDT- 2020/01/28 06:00
PHST- 2019/09/23 00:00 [received]
PHST- 2019/11/11 00:00 [revised]
PHST- 2019/12/26 00:00 [accepted]
PHST- 2020/01/28 06:00 [pubmed]
PHST- 2020/07/15 06:00 [medline]
PHST- 2020/01/28 06:00 [entrez]
PHST- 2021/03/18 00:00 [pmc-release]
AID - S0896-6273(19)31097-9 [pii]
AID - 10.1016/j.neuron.2019.12.031 [doi]
PST - ppublish
SO  - Neuron. 2020 Mar 18;105(6):1027-1035.e2. doi: 10.1016/j.neuron.2019.12.031. Epub 
      2020 Jan 23.