PMID- 31986422
OWN - NLM
STAT- MEDLINE
DCOM- 20210106
LR  - 20210106
IS  - 1532-8198 (Electronic)
IS  - 1092-9134 (Linking)
VI  - 45
DP  - 2020 Apr
TI  - Maspin expression and anti-apoptotic pathway regulation by bcl2 in laryngeal 
      cancer.
PG  - 151471
LID - S1092-9134(20)30012-5 [pii]
LID - 10.1016/j.anndiagpath.2020.151471 [doi]
AB  - OBJECTIVES: Comprehension of the interplay of pro-apoptotic and anti-apoptotic 
      stimuli in laryngeal squamous cell carcinoma (LSCC) is crucial to understand 
      tumor development, biological behavior and treatment response. Bcl-2 family 
      proteins mainly regulate the apoptotic signal cascade. In some cancers, maspin 
      seems to influence the balance between pro-apoptosis and anti-apoptosis bcl-2 
      family proteins. The aim of this study was to investigate the potential 
      relationship between bcl-2 anti-apoptotic factor and the tumor suppressor maspin 
      in LSCC. MATERIALS AND METHODS: 31 consecutive patients who underwent primary 
      surgery and post-operative radiotherapy for LSCC were evaluated retrospectively. 
      For each case, immunohistochemistry assays for bcl-2 and maspin were performed. 
      Data were also collected on N-status, pT stage, grading, recurrence and 
      disease-free survival (DFS). RESULTS: Patients with nuclear maspin pattern of 
      expression showed a significantly lower recurrence rate (p = 0.04) and longer DFS 
      (p = 0.0018). The expression of bcl-2 was not associated with recurrence rate or 
      DFS either in the whole cohort or in cases with nuclear maspin pattern, while in 
      patients with non-nuclear maspin pattern, a statistical trend was found toward a 
      shorter DFS for bcl-2 positive cases (p = 0.062). In the multivariate model, only 
      maspin expression pattern retained its independent prognostic significance 
      (p = 0.006). CONCLUSIONS: Nuclear maspin pattern seemed to be an independent 
      positive prognostic factor, while bcl-2 prognostic value was related to maspin 
      expression pattern. Further investigations are needed to support the use of bcl-2 
      inhibitors in multimodality or multitarget strategies against advanced LSCCs, 
      also considering the role and expression of tumor suppressor genes.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Lovato, Andrea
AU  - Lovato A
AD  - Department of Neuroscience DNS, Audiology Unit, University of Padova, Treviso, 
      Italy.
FAU - Franz, Leonardo
AU  - Franz L
AD  - Department of Neuroscience DNS, Otolaryngology Section, University of Padova, 
      Padova, Italy.
FAU - Carraro, Valentina
AU  - Carraro V
AD  - Department of Medicine DIMED, University of Padova, Padova, Italy.
FAU - Bandolin, Luigia
AU  - Bandolin L
AD  - Department of Neuroscience DNS, Otolaryngology Section, University of Padova, 
      Padova, Italy.
FAU - Contro, Giacomo
AU  - Contro G
AD  - Department of Neuroscience DNS, Otolaryngology Section, University of Padova, 
      Padova, Italy.
FAU - Ottaviano, Giancarlo
AU  - Ottaviano G
AD  - Department of Neuroscience DNS, Otolaryngology Section, University of Padova, 
      Padova, Italy.
FAU - de Filippis, Cosimo
AU  - de Filippis C
AD  - Department of Neuroscience DNS, Audiology Unit, University of Padova, Treviso, 
      Italy.
FAU - Blandamura, Stella
AU  - Blandamura S
AD  - Department of Medicine DIMED, University of Padova, Padova, Italy.
FAU - Alessandrini, Lara
AU  - Alessandrini L
AD  - Department of Medicine DIMED, University of Padova, Padova, Italy.
FAU - Marioni, Gino
AU  - Marioni G
AD  - Department of Neuroscience DNS, Otolaryngology Section, University of Padova, 
      Padova, Italy. Electronic address: gino.marioni@unipd.it.
LA  - eng
PT  - Journal Article
DEP - 20200120
PL  - United States
TA  - Ann Diagn Pathol
JT  - Annals of diagnostic pathology
JID - 9800503
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (BCL2 protein, human)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (SERPIN-B5)
RN  - 0 (Serpins)
SB  - IM
MH  - Aged
MH  - Apoptosis/*genetics
MH  - Apoptosis Regulatory Proteins/genetics
MH  - Carcinoma, Squamous Cell/*genetics/radiotherapy/surgery
MH  - Disease-Free Survival
MH  - Female
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Laryngeal Neoplasms/*pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Grading/methods
MH  - Neoplasm Recurrence, Local/pathology
MH  - Prognosis
MH  - Proto-Oncogene Proteins c-bcl-2/*genetics/metabolism
MH  - Retrospective Studies
MH  - Serpins/*genetics
OTO - NOTNLM
OT  - Apoptosis
OT  - Bcl-2
OT  - Laryngeal squamous cell carcinoma
OT  - Maspin pattern
OT  - Prognosis
COIS- Declaration of competing interest The authors declare that they have no conflict 
      of interest.
EDAT- 2020/01/28 06:00
MHDA- 2021/01/07 06:00
CRDT- 2020/01/28 06:00
PHST- 2019/10/22 00:00 [received]
PHST- 2019/11/25 00:00 [revised]
PHST- 2020/01/09 00:00 [accepted]
PHST- 2020/01/28 06:00 [pubmed]
PHST- 2021/01/07 06:00 [medline]
PHST- 2020/01/28 06:00 [entrez]
AID - S1092-9134(20)30012-5 [pii]
AID - 10.1016/j.anndiagpath.2020.151471 [doi]
PST - ppublish
SO  - Ann Diagn Pathol. 2020 Apr;45:151471. doi: 10.1016/j.anndiagpath.2020.151471. 
      Epub 2020 Jan 20.