PMID- 31987502
OWN - NLM
STAT- MEDLINE
DCOM- 20201016
LR  - 20201016
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 524
IP  - 2
DP  - 2020 Apr 2
TI  - beta-catenin stimulates Tcf7l1 degradation through recruitment of casein kinase 2 in 
      mouse embryonic stem cells.
PG  - 280-287
LID - S0006-291X(20)30143-1 [pii]
LID - 10.1016/j.bbrc.2020.01.074 [doi]
AB  - Activation of the Wnt/beta-catenin signaling pathway by the inhibition of glycogen 
      synthase kinase-3 (GSK-3) will induce Tcf7l1 protein degradation to effectively 
      promote embryonic stem cell (ESC) self-renewal. However, the exact mechanism 
      remains unclear. Here, we found that inhibition of casein kinase 2 (Csnk2) by TBB 
      or DMAT was sufficient to block the reduction of the Tcf7l1 protein induced by 
      CHIR99021, a specific inhibitor of GSK-3. Similarly, downregulation of Csnk2 
      increased the Tcf7l1 level. In contrast, overexpression of Csnk2 significantly 
      decreased Tcf7l1 protein stability in mouse ESCs. Notably, Csnk2alpha1 controls 
      Tcf7l1 turnover to a greater degree than the other two isoforms of Csnk2, Csnk2alpha2 
      and Csnk2beta, as Csnk2alpha1-overexpressing mouse ESCs exhibited the lowest level of 
      Tcf7l1. Csnk2alpha1 interacted with and phosphorylated Tcf7l1. In addition, the 
      association of Csnk2alpha1 and Tcf7l1 was enhanced by CHIR99021. Our study 
      demonstrated, for the first time, that Csnk2 is involved in Tcf7l1 turnover 
      mediated by the Wnt/beta-catenin signaling pathway. These results expand our 
      understanding of the function and circuit of Wnt/beta-catenin signaling pathway in 
      ESCs.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences & 
      Institute of Physical Science and Information Technology, Anhui University, 
      Hefei, 230601, PR China.
FAU - Zhu, Zhenhua
AU  - Zhu Z
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences & 
      Institute of Physical Science and Information Technology, Anhui University, 
      Hefei, 230601, PR China.
FAU - Ding, Huiwen
AU  - Ding H
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences & 
      Institute of Physical Science and Information Technology, Anhui University, 
      Hefei, 230601, PR China.
FAU - Wan, Shengpeng
AU  - Wan S
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences & 
      Institute of Physical Science and Information Technology, Anhui University, 
      Hefei, 230601, PR China.
FAU - Zhang, Xinbao
AU  - Zhang X
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences & 
      Institute of Physical Science and Information Technology, Anhui University, 
      Hefei, 230601, PR China.
FAU - Li, Yuting
AU  - Li Y
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences & 
      Institute of Physical Science and Information Technology, Anhui University, 
      Hefei, 230601, PR China.
FAU - Ji, Junxiang
AU  - Ji J
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences & 
      Institute of Physical Science and Information Technology, Anhui University, 
      Hefei, 230601, PR China.
FAU - Wang, Xin
AU  - Wang X
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences & 
      Institute of Physical Science and Information Technology, Anhui University, 
      Hefei, 230601, PR China.
FAU - Zhang, Meng
AU  - Zhang M
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences & 
      Institute of Physical Science and Information Technology, Anhui University, 
      Hefei, 230601, PR China.
FAU - Ye, Shou-Dong
AU  - Ye SD
AD  - Center for Stem Cell and Translational Medicine, School of Life Sciences & 
      Institute of Physical Science and Information Technology, Anhui University, 
      Hefei, 230601, PR China. Electronic address: shdye@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200125
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Tcf7l1 protein, mouse)
RN  - 0 (Transcription Factor 7-Like 1 Protein)
RN  - 0 (beta Catenin)
RN  - EC 2.7.11.1 (Casein Kinase II)
RN  - EC 2.7.11.1 (Csnk2a1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Casein Kinase II/*metabolism
MH  - Cell Line
MH  - Mice
MH  - Mouse Embryonic Stem Cells/*metabolism
MH  - Protein Interaction Maps
MH  - Proteolysis
MH  - Transcription Factor 7-Like 1 Protein/*metabolism
MH  - beta Catenin/*metabolism
OTO - NOTNLM
OT  - Csnk2
OT  - Degradation
OT  - Embryonic stem cells
OT  - Tcf7l1
OT  - beta-catenin
COIS- Declaration of competing interest None.
EDAT- 2020/01/29 06:00
MHDA- 2020/10/21 06:00
CRDT- 2020/01/29 06:00
PHST- 2019/12/09 00:00 [received]
PHST- 2020/01/13 00:00 [accepted]
PHST- 2020/01/29 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2020/01/29 06:00 [entrez]
AID - S0006-291X(20)30143-1 [pii]
AID - 10.1016/j.bbrc.2020.01.074 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2020 Apr 2;524(2):280-287. doi: 
      10.1016/j.bbrc.2020.01.074. Epub 2020 Jan 25.