PMID- 31988622 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200929 IS - 1344-7610 (Print) IS - 1347-3735 (Electronic) IS - 1344-7610 (Linking) VI - 69 IP - 4 DP - 2019 Dec TI - Construction of a genetic linkage map of Lentinula edodes based on SSR, SRAP and TRAP markers. PG - 585-591 LID - 10.1270/jsbbs.18123 [doi] AB - Genetic mapping is a basic tool for eukaryotic genomic research. It allows the localization of genes or quantitative trait loci (QTLs) and map-based cloning. In this study, we constructed a linkage map based on DNA samples from a commercial strain L808, including two parental monokaryons and 93 single spore isolates considered with segregating to 1:1:1:1 at four mating types (A(1)B(1), A(1)B(2), A(2)B(1) and A(2)B(2)). Using Simple Sequence Repeats (SSR), Sequence Related Amplified Polymorphism (SRAP), Target Region Amplified Polymorphism (TRAP) molecular markers, 182 molecular markers and two mating factors were located on 11 linkage groups (LGs). The total length of the map was 948.083 centimorgan (cM), with an average marker interval distance of 4.817 cM. Only two gaps spanning more than 20 cM was observed. The probability of 20 cM, 10 cM, 5 cM genetic distance cover one marker was 99.68%, 94.36%, 76.43% in our genetic linkage map, respectively. This is the first linkage map of Lentinula edodes using SSR markers, which provides essential information for quantitative trait analyses and improvement of genome assembly. CI - Copyright (c) 2019 by JAPANESE SOCIETY OF BREEDING. FAU - Dong, Hui AU - Dong H AD - Institute of Agro-Food Quality Standard and Testing Technology, Shanghai Academy of Agricultural Sciences, Laboratory of Agro-Food Quality and Safety Risk Assessment at Shanghai, Shanghai, 201403, China. FAU - Shang, Xiaodong AU - Shang X AD - Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Research Center of Edible Fungi Biotechnology and Engineering, Shanghai, 201403, China. FAU - Zhao, Xiaoyan AU - Zhao X AD - Institute of Agro-Food Quality Standard and Testing Technology, Shanghai Academy of Agricultural Sciences, Laboratory of Agro-Food Quality and Safety Risk Assessment at Shanghai, Shanghai, 201403, China. FAU - Yu, Hailong AU - Yu H AD - Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Research Center of Edible Fungi Biotechnology and Engineering, Shanghai, 201403, China. FAU - Jiang, Ning AU - Jiang N AD - Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Research Center of Edible Fungi Biotechnology and Engineering, Shanghai, 201403, China. FAU - Zhang, Meiyan AU - Zhang M AD - Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Research Center of Edible Fungi Biotechnology and Engineering, Shanghai, 201403, China. FAU - Tan, Qi AU - Tan Q AD - Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Research Center of Edible Fungi Biotechnology and Engineering, Shanghai, 201403, China. FAU - Zhou, Changyan AU - Zhou C AD - Institute of Agro-Food Quality Standard and Testing Technology, Shanghai Academy of Agricultural Sciences, Laboratory of Agro-Food Quality and Safety Risk Assessment at Shanghai, Shanghai, 201403, China. FAU - Zhang, Lujun AU - Zhang L AD - Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, National Research Center of Edible Fungi Biotechnology and Engineering, Shanghai, 201403, China. LA - eng PT - Journal Article DEP - 20190919 PL - Japan TA - Breed Sci JT - Breeding science JID - 9888571 PMC - PMC6977451 OTO - NOTNLM OT - Lentinula edodes OT - SSR OT - genetic linkage map EDAT- 2020/01/29 06:00 MHDA- 2020/01/29 06:01 PMCR- 2019/12/01 CRDT- 2020/01/29 06:00 PHST- 2018/08/15 00:00 [received] PHST- 2019/07/01 00:00 [accepted] PHST- 2020/01/29 06:00 [entrez] PHST- 2020/01/29 06:00 [pubmed] PHST- 2020/01/29 06:01 [medline] PHST- 2019/12/01 00:00 [pmc-release] AID - 69_18123 [pii] AID - 10.1270/jsbbs.18123 [doi] PST - ppublish SO - Breed Sci. 2019 Dec;69(4):585-591. doi: 10.1270/jsbbs.18123. Epub 2019 Sep 19.