PMID- 31989429
OWN - NLM
STAT- MEDLINE
DCOM- 20201019
LR  - 20210216
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 47
IP  - 3
DP  - 2020 Mar
TI  - Detection of antibiotic resistance profiles and aminoglycoside-modifying enzyme 
      (AME) genes in high-level aminoglycoside-resistant (HLAR) enterococci isolated 
      from raw milk and traditional cheeses in Turkey.
PG  - 1703-1712
LID - 10.1007/s11033-020-05262-4 [doi]
AB  - The aim of this study was isolation and identification of the high-level 
      aminoglycoside-resistant (HLAR) enterococci in raw milk and dairy products and to 
      analyze their antibiotic resistance and the presence of aminoglycoside-modifying 
      enzyme (AME) genes. A total of 59 HLAR enterococci were isolated from raw milk 
      and traditional cheese samples. Thirty-nine of the 59 HLAR enterococci were 
      isolated on streptomycin-containing agar medium, while the other 20 HLAR strains 
      were isolated on gentamicin containing agar medium. The 59 HLAR enterococci were 
      identified as 26 E. faecalis (44.07%), 18 E. faecium (30.51%), 13 E. durans 
      (22.03%), and two E. gallinarum (3.39%) by species-specific PCR. Disk diffusion 
      tests showed that teicoplanin were the most effective antibiotics used in this 
      study, while 89.83% of isolates were found to be resistant to tetracycline. High 
      rates of multiple antibiotic resistance were detected in HLAR isolates. Minimum 
      inhibitory concentration (MIC) values of HLAR enterococci against streptomycin 
      and gentamicin were found in the range of 64 to > 4096 microg/mL. Forty-seven 
      (79.66%) of the 59 HLAR enterococci were found to be both high-level 
      streptomycin-resistant (HLSR) and high-level gentamicin-resistant (HLGR) by MIC 
      tests. However, no correlation was found between the results of the disk 
      diffusion and MIC tests for gentamicin and streptomycin in some HLAR strains. The 
      aph(3')-IIIa (94.92%) was found to be most prevalent AME gene followed by 
      ant(4')-Ia (45.76%), ant(6')-Ia (20.34%) and aph(2'')-Ic (10.17%). None of the 
      isolates contained the aac(6')-Ie-aph(2'')-Ia, aph(2'')-Ib or aph(2'')-Id genes. 
      None of the AME-encoding genes were identified in E. durans RG20.1, E. faecalis 
      RG22.4, or RG26.1. In conclusion, HLAR enterococci strains isolated in this study 
      may act as reservoirs in the dissemination of antibiotic resistance genes.
FAU - Ozdemir, Rahime
AU  - Ozdemir R
AD  - Department of Food Engineering, Faculty of Engineering, Suleyman Demirel 
      University, Isparta, Turkey.
FAU - Tuncer, Yasin
AU  - Tuncer Y
AUID- ORCID: 0000-0002-2075-5027
AD  - Department of Food Engineering, Faculty of Engineering, Suleyman Demirel 
      University, Isparta, Turkey. yasintuncer@sdu.edu.tr.
LA  - eng
GR  - 4739-YL1-16/Suleyman Demirel Universitesi/
PT  - Journal Article
DEP - 20200127
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Aminoglycosides)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Gentamicins)
RN  - 61036-62-2 (Teicoplanin)
RN  - EC 2.7.1.95 (Kanamycin Kinase)
RN  - Y45QSO73OB (Streptomycin)
SB  - IM
MH  - Aminoglycosides/*pharmacology
MH  - Animals
MH  - Bacterial Proteins/*genetics/metabolism
MH  - Cheese/*microbiology
MH  - Drug Resistance, Bacterial/*genetics
MH  - Drug Resistance, Multiple, Bacterial/*genetics
MH  - Enterococcus/classification/*genetics/metabolism
MH  - Gentamicins/pharmacology
MH  - Humans
MH  - Kanamycin Kinase/genetics/metabolism
MH  - Microbial Sensitivity Tests/methods
MH  - Milk/*microbiology
MH  - Streptomycin/pharmacology
MH  - Teicoplanin/pharmacology
MH  - Turkey
OTO - NOTNLM
OT  - Aminoglycoside-modifying enzymes
OT  - Cheese
OT  - Enterococcus
OT  - High-level aminoglycoside resistance
OT  - Raw milk
OT  - aph(3')-IIIa
EDAT- 2020/01/29 06:00
MHDA- 2020/10/21 06:00
CRDT- 2020/01/29 06:00
PHST- 2019/07/31 00:00 [received]
PHST- 2020/01/18 00:00 [accepted]
PHST- 2020/01/04 00:00 [revised]
PHST- 2020/01/29 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2020/01/29 06:00 [entrez]
AID - 10.1007/s11033-020-05262-4 [pii]
AID - 10.1007/s11033-020-05262-4 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2020 Mar;47(3):1703-1712. doi: 10.1007/s11033-020-05262-4. Epub 
      2020 Jan 27.