PMID- 31989773
OWN - NLM
STAT- MEDLINE
DCOM- 20200731
LR  - 20200731
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 128
IP  - 6
DP  - 2020 Jun
TI  - Diversity and abundance of comammox bacteria in the sediments of an urban lake.
PG  - 1647-1657
LID - 10.1111/jam.14593 [doi]
AB  - AIMS: Although comammox have been discovered in a variety of ecosystems, there 
      are few studies in urban lakes. This paper attempted to confirm whether this 
      ammonia-oxidizing microbe exists in urban lakes and to determine the factors 
      influencing its existence. METHODS AND RESULTS: This study investigated the 
      diversity and abundance of comammox bacteria in sediments of a typical urban lake 
      in China, and their ecological relationship with other ammonia-oxidizing 
      micro-organisms. The phylogenetic analysis indicated that comammox clade A 
      existed in the sediment of Lake Donghu, and the comammox bacteria co-existed with 
      ammonia-oxidizing archaea (AOA), ammonia-oxidizing bacteria (AOB) and anaerobic 
      ammonia-oxidizing (anammox) bacteria in the sediment of this lake. The abundances 
      of the ammonia monooxygenase subunit A (amoA) genes for comammox, AOA, AOB and 
      anammox 16S rRNA were 2.43 x 10(8) , 1.07 x 10(8) , 3.24 x 10(7) and 
      3.21 x 10(11) copies per gram dry sediment respectively. Moreover, the amoA gene 
      abundance of comammox was positively correlated with that of AOA and AOB. The 
      redundancy analysis showed that the abundance of the comammox amoA gene was 
      negatively correlated with the concentration of main indicators for nitrogen 
      status in both the sediment and the water column, indicating that eutrophication 
      may inhibit the growth of comammox bacteria. CONCLUSIONS: Comammox bacteria play 
      an important ecological role in the nitrogen cycle of urban lake sediments. 
      SIGNIFICANCE AND IMPACT OF THE STUDY: Our results indicated comammox bacteria 
      were widespread in urban lakes and eutrophication may inhibit their growth.
CI  - (c) 2020 The Society for Applied Microbiology.
FAU - Xu, Y
AU  - Xu Y
AD  - Laboratory of Eco-Environmental Engineering Research of Huazhong Agricultural 
      University, Wuhan, China.
AD  - Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze 
      River), Ministry of Agriculture, Wuhan, China.
FAU - Lu, J
AU  - Lu J
AD  - Australian Rivers Institute, Griffith University, Nathan, Qld, Australia.
FAU - Wang, Y
AU  - Wang Y
AD  - State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, 
      Beijing, China.
AD  - Department of Water Environment, China Institute of Water Resources and 
      Hydropower Research, Beijing, China.
FAU - Liu, G
AU  - Liu G
AD  - Laboratory of Eco-Environmental Engineering Research of Huazhong Agricultural 
      University, Wuhan, China.
AD  - Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze 
      River), Ministry of Agriculture, Wuhan, China.
FAU - Wan, X
AU  - Wan X
AD  - Laboratory of Eco-Environmental Engineering Research of Huazhong Agricultural 
      University, Wuhan, China.
AD  - Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze 
      River), Ministry of Agriculture, Wuhan, China.
FAU - Hua, Y
AU  - Hua Y
AD  - Laboratory of Eco-Environmental Engineering Research of Huazhong Agricultural 
      University, Wuhan, China.
AD  - Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze 
      River), Ministry of Agriculture, Wuhan, China.
FAU - Zhu, D
AU  - Zhu D
AD  - Laboratory of Eco-Environmental Engineering Research of Huazhong Agricultural 
      University, Wuhan, China.
AD  - Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze 
      River), Ministry of Agriculture, Wuhan, China.
FAU - Zhao, J
AU  - Zhao J
AUID- ORCID: 0000-0003-1465-6134
AD  - Laboratory of Eco-Environmental Engineering Research of Huazhong Agricultural 
      University, Wuhan, China.
AD  - Key Laboratory of Arable Land Conservation (Middle and Lower Reaches of Yangtze 
      River), Ministry of Agriculture, Wuhan, China.
LA  - eng
SI  - GENBANK/MK948223
SI  - GENBANK/MK948291
GR  - 41371452, U1802241, 91647208/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20200214
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 7664-41-7 (Ammonia)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.3.- (ammonia monooxygenase)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Anaerobiosis
MH  - Archaea/classification/genetics/growth & development/metabolism
MH  - Bacteria/classification/genetics/growth & development/*metabolism
MH  - China
MH  - Ecosystem
MH  - Eutrophication
MH  - Geologic Sediments/*microbiology
MH  - Lakes/chemistry/*microbiology
MH  - Nitrogen Cycle
MH  - Oxidation-Reduction
MH  - Oxidoreductases/genetics
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
OTO - NOTNLM
OT  - abundance
OT  - ammonia-oxidizing micro-organisms
OT  - comammox
OT  - diversity
OT  - sediment
OT  - urban lake
EDAT- 2020/01/29 06:00
MHDA- 2020/08/01 06:00
CRDT- 2020/01/29 06:00
PHST- 2019/08/30 00:00 [received]
PHST- 2020/01/19 00:00 [revised]
PHST- 2020/01/21 00:00 [accepted]
PHST- 2020/01/29 06:00 [pubmed]
PHST- 2020/08/01 06:00 [medline]
PHST- 2020/01/29 06:00 [entrez]
AID - 10.1111/jam.14593 [doi]
PST - ppublish
SO  - J Appl Microbiol. 2020 Jun;128(6):1647-1657. doi: 10.1111/jam.14593. Epub 2020 
      Feb 14.