PMID- 31990133
OWN - NLM
STAT- MEDLINE
DCOM- 20200804
LR  - 20200804
IS  - 1099-1565 (Electronic)
IS  - 0958-0344 (Linking)
VI  - 31
IP  - 5
DP  - 2020 Sep
TI  - A feasible UHPLC-MS/MS method for concurrent quantification of 10 bioactive 
      principles in Aquilaria leaf tea by the multiple reaction monitoring analytical 
      mode.
PG  - 583-593
LID - 10.1002/pca.2923 [doi]
AB  - INTRODUCTION: Recently, the fresh leaves of Aquilaria trees have been processed 
      as food products such as agarwood tea due to its beneficial medicinal properties. 
      However, there have not been any reported analytical methods to quantify the 
      bioactive principles in the processed products. OBJECTIVE: A rapid and sensitive 
      ultrahigh-performance liquid chromatography (UHPLC) coupled with electrospray 
      ionisation (ESI) tandem mass spectrometry (MS/MS) method was developed and 
      validated for the simultaneous determination of 10 bioactive components in 
      Aquilaria leaf tea. METHODS: The UHPLC-MS/MS was used for quantification operated 
      in multiple reaction monitoring (MRM) mode. The optimised chromatographic 
      parameters were conducted on a Shim-pack XR-ODS II column and mobile phases 
      consisted of acetonitrile and 0.1% formic acid in water. RESULTS: All the samples 
      were analysed within 20 min. The established method showed excellent linearity 
      (R(2) > 0.9988), good repeatability with all the relative standard deviation 
      values lower than 3.27%, and satisfactory recovery (79.72-119.22%). The matrix 
      effect factors ranged from 87.65 to 97.27% in the examination. The developed 
      method was applied to the determination of 10 bioactive principles (1-10) in six 
      different Aquilaria leaf tea samples. Among the analytes, mangiferin (1) and 
      iriflophenone 2-O-alpha-l-rhamnopyranoside (2) were the most abundant compounds in 
      the extracts of Aquilaria leaf tea, and it indicated that these biotech products 
      may possess laxative effects. CONCLUSION: This proposed method appeared to be a 
      useful tool for the quality control of commercial products of Aquilaria leaf tea 
      and thus provided an analytical reference for herbal drinks.
CI  - (c) 2020 John Wiley & Sons, Ltd.
FAU - Kuo, Ping-Chung
AU  - Kuo PC
AUID- ORCID: 0000-0003-3019-1138
AD  - School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan, 
      701, Taiwan.
FAU - Li, Yue-Chiun
AU  - Li YC
AD  - School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan, 
      701, Taiwan.
AD  - Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, 
      402, Taiwan.
FAU - Yang, Mei-Lin
AU  - Yang ML
AD  - School of Pharmacy, College of Medicine, National Cheng Kung University, Tainan, 
      701, Taiwan.
FAU - Tzen, Jason T C
AU  - Tzen JTC
AD  - Graduate Institute of Biotechnology, National Chung-Hsing University, Taichung, 
      402, Taiwan.
LA  - eng
GR  - MOST/Ministry of Science and Technology, Taiwan/
PT  - Journal Article
DEP - 20200128
PL  - England
TA  - Phytochem Anal
JT  - Phytochemical analysis : PCA
JID - 9200492
RN  - 0 (Tea)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Plant Leaves
MH  - Reproducibility of Results
MH  - *Tandem Mass Spectrometry
MH  - Tea
MH  - *Thymelaeaceae
OTO - NOTNLM
OT  - accuracy
OT  - agarwood tea
OT  - multiple reaction monitoring
OT  - precision
EDAT- 2020/01/29 06:00
MHDA- 2020/08/05 06:00
CRDT- 2020/01/29 06:00
PHST- 2019/12/22 00:00 [received]
PHST- 2020/01/11 00:00 [revised]
PHST- 2020/01/13 00:00 [accepted]
PHST- 2020/01/29 06:00 [pubmed]
PHST- 2020/08/05 06:00 [medline]
PHST- 2020/01/29 06:00 [entrez]
AID - 10.1002/pca.2923 [doi]
PST - ppublish
SO  - Phytochem Anal. 2020 Sep;31(5):583-593. doi: 10.1002/pca.2923. Epub 2020 Jan 28.