PMID- 31993168
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240328
IS  - 2048-7177 (Print)
IS  - 2048-7177 (Electronic)
IS  - 2048-7177 (Linking)
VI  - 8
IP  - 1
DP  - 2020 Jan
TI  - Camellia (Camellia oleifera Abel.) seed oil promotes milk fat and protein 
      synthesis-related gene expression in bovine mammary epithelial cells.
PG  - 419-427
LID - 10.1002/fsn3.1326 [doi]
AB  - Camellia (Camellia oleifera Abel.) seed oil is a commonly used edible oil of 
      China. In ancient Chinese literature, it is mentioned to be helpful for 
      postpartum repair and lactation in women. Research on camellia seed oil (CO) as a 
      feed additive for dairy cattle is less. We investigated the effect of CO on the 
      expression of milk fat and protein syntheses-related genes in differentiated 
      bovine mammary epithelial cells (MAC-T) using soybean oil (SO) as the control. 
      The results showed that CO increased the expression of genes related to de novo 
      synthesis of fatty acids including sterol regulatory element-binding protein 1 
      (SREBP1), acetyl-CoA carboxylase 1 (ACC), fatty acid synthase (FASN), lipoprotein 
      lipase (LPL), and stearoyl-CoA desaturase (SCD) (p < .05). Among the milk protein 
      genes analyzed, CO increased beta-casein mRNA expression (p < .05) and decreased 
      alphaS1-casein mRNA expression (p < .05) in MAC-T cells. CO upregulated the pathways 
      related to milk protein synthesis with increased mRNA levels of phosphoinositide 
      3-kinase (PI3K), RAC-alpha serine/threonine-protein kinase (AKT1), and mammalian 
      target of rapamycin (mTOR) (p < .05) in MAC-T cells. Ribosomal protein S6 kinase 
      beta-1 (S6K1) gene was upregulated, and eukaryotic initiation factor 4E (eIF4E) 
      gene (p < .05) was downregulated with CO treatment. The mRNA expression levels of 
      janus kinase 2 (JAK2), activator of transcription 5-beta (STAT5-beta), and E74-like 
      factor 5 (ELF5) were elevated in MAC-T cells treated with CO (p < .05). 
      Meanwhile, the protein expression levels of S6K1, STAT5-beta, phosphorylated mTOR 
      (p-mTOR), p-S6K1, and p-STAT5-beta increased in MAC-T cells treated with CO 
      (p < .05). In summary, CO promoted beta-casein synthesis by regulating 
      PI3K-mTOR-S6K1 and JAK2-STAT5 signaling pathways and influenced fatty acid 
      synthesis by regulating SREBP1-related genes in MAC-T cells. We need to further 
      confirm the function of CO using in vivo models.
CI  - (c) 2019 The Authors. Food Science & Nutrition published by Wiley Periodicals, Inc.
FAU - Zhong, Wanqi
AU  - Zhong W
AUID- ORCID: 0000-0002-9086-5133
AD  - Department of Animal Science College of Animal Science Jilin University Changchun 
      China.
FAU - Shen, Jinglin
AU  - Shen J
AUID- ORCID: 0000-0003-1921-4407
AD  - Department of Animal Science College of Animal Science Jilin University Changchun 
      China.
FAU - Liao, Xiandong
AU  - Liao X
AUID- ORCID: 0000-0002-8851-1715
AD  - Department of Animal Science College of Animal Science Jilin University Changchun 
      China.
FAU - Liu, Xinlu
AU  - Liu X
AUID- ORCID: 0000-0003-1257-9964
AD  - Department of Animal Science College of Animal Science Jilin University Changchun 
      China.
FAU - Zhang, Jing
AU  - Zhang J
AUID- ORCID: 0000-0002-4823-4629
AD  - Department of Animal Science College of Animal Science Jilin University Changchun 
      China.
FAU - Zhou, Changhai
AU  - Zhou C
AUID- ORCID: 0000-0003-4750-2107
AD  - Department of Animal Science College of Animal Science Jilin University Changchun 
      China.
FAU - Jin, Yongcheng
AU  - Jin Y
AUID- ORCID: 0000-0002-3462-3674
AD  - Department of Animal Science College of Animal Science Jilin University Changchun 
      China.
LA  - eng
PT  - Journal Article
DEP - 20191205
PL  - United States
TA  - Food Sci Nutr
JT  - Food science & nutrition
JID - 101605473
PMC - PMC6977417
OTO - NOTNLM
OT  - bovine mammary epithelial cells
OT  - camellia seed oil
OT  - casein
OT  - fatty acids
OT  - mRNA expression
OT  - protein expression
COIS- The authors declare that they do not have any conflict of interest.
EDAT- 2020/01/30 06:00
MHDA- 2020/01/30 06:01
PMCR- 2019/12/05
CRDT- 2020/01/30 06:00
PHST- 2019/10/26 00:00 [received]
PHST- 2019/11/15 00:00 [revised]
PHST- 2019/11/18 00:00 [accepted]
PHST- 2020/01/30 06:00 [entrez]
PHST- 2020/01/30 06:00 [pubmed]
PHST- 2020/01/30 06:01 [medline]
PHST- 2019/12/05 00:00 [pmc-release]
AID - FSN31326 [pii]
AID - 10.1002/fsn3.1326 [doi]
PST - epublish
SO  - Food Sci Nutr. 2019 Dec 5;8(1):419-427. doi: 10.1002/fsn3.1326. eCollection 2020 
      Jan.