PMID- 31995234
OWN - NLM
STAT- MEDLINE
DCOM- 20200625
LR  - 20200625
IS  - 1095-8649 (Electronic)
IS  - 0022-1112 (Linking)
VI  - 96
IP  - 3
DP  - 2020 Mar
TI  - Metabolic and immune responses of Chinook salmon (Oncorhynchus tshawytscha) 
      smolts to a short-term poly (I:C) challenge.
PG  - 731-746
LID - 10.1111/jfb.14266 [doi]
AB  - Polyinosinic:polycytidylic acid [poly (I:C)] was administered in vivo to Chinook 
      salmon (Oncorhynchus tshawytscha) post-smolts to determine the immune responses 
      on haematological and cellular functional parameters, including spleen (SP), head 
      kidney (HK) and red blood cell (RBC) cytokine expression, as well as serum 
      metabolomics. Poly (I:C) in vivo (24 h exposure) did not affect fish 
      haematological parameters, leucocyte phagocytic activity and phagocytic index, 
      reactive oxygen species and nitric oxide production. Gas chromatography-mass 
      spectrometry-based metabolomics revealed that poly (I:C) significantly altered 
      the serum biochemistry profile of 25 metabolites. Metabolites involved in the 
      branched-chain amino acid/glutathione and transsulphuration pathways and 
      phospholipid metabolism accumulated in poly (I:C)-treated fish, whereas those 
      involved in the glycolytic and energy metabolism pathways were downregulated. At 
      cytokine transcript level, poly (I:C) induced a significant upregulation of 
      antiviral ifngamma in HK and Mx1 protein in HK, SP and RBCs. This study provides 
      evidence for poly (I:C)-induced, immune-related biomarkers at metabolic and 
      molecular levels in farmed O. tshawytscha in vivo. These findings provide 
      insights into short-term effects of poly (I:C) at haematological, innate and 
      adaptive immunity and metabolic levels, setting the stage for future studies.
CI  - (c) 2020 The Fisheries Society of the British Isles.
FAU - Lulijwa, Ronald
AU  - Lulijwa R
AD  - Aquaculture Biotechnology Research Group, School of Science, Faculty of Health 
      and Environmental Sciences, Auckland University of Technology, Auckland, New 
      Zealand.
AD  - National Agricultural Research Organisation (NARO), Rwebitaba Zonal Agricultural 
      Research and Development Institute (Rwebitaba-ZARDI), Fort Portal, Uganda.
FAU - Alfaro, Andrea C
AU  - Alfaro AC
AUID- ORCID: 0000-0003-0543-7212
AD  - Aquaculture Biotechnology Research Group, School of Science, Faculty of Health 
      and Environmental Sciences, Auckland University of Technology, Auckland, New 
      Zealand.
FAU - Merien, Fabrice
AU  - Merien F
AD  - Aquaculture Biotechnology Research Group, School of Science, Faculty of Health 
      and Environmental Sciences, Auckland University of Technology, Auckland, New 
      Zealand.
AD  - AUT-Roche Diagnostics Laboratory, School of Science, Faculty of Health and 
      Environmental Sciences, Auckland University of Technology, Auckland, New Zealand.
FAU - Burdass, Mark
AU  - Burdass M
AD  - Nelson Marlborough Institute of Technology (NMIT), Nelson, New Zealand.
FAU - Meyer, Jill
AU  - Meyer J
AD  - Aquaculture Biotechnology Research Group, School of Science, Faculty of Health 
      and Environmental Sciences, Auckland University of Technology, Auckland, New 
      Zealand.
AD  - AUT-Roche Diagnostics Laboratory, School of Science, Faculty of Health and 
      Environmental Sciences, Auckland University of Technology, Auckland, New Zealand.
FAU - Venter, Leonie
AU  - Venter L
AD  - Aquaculture Biotechnology Research Group, School of Science, Faculty of Health 
      and Environmental Sciences, Auckland University of Technology, Auckland, New 
      Zealand.
FAU - Young, Tim
AU  - Young T
AD  - Aquaculture Biotechnology Research Group, School of Science, Faculty of Health 
      and Environmental Sciences, Auckland University of Technology, Auckland, New 
      Zealand.
AD  - The Centre for Biomedical and Chemical Sciences, School of Science, Faculty of 
      Health and Environmental Sciences, Auckland University of Technology, Auckland, 
      New Zealand.
LA  - eng
GR  - This project was funded by a New Zealand AID doctoral scholarship to Ronald 
      Lulijwa under the supervision of Andrea C. Alfaro and Fabrice Merien and 
      co-funded by AUT's Aquaculture Biotechnology Research Group (ABRG)./
PT  - Journal Article
DEP - 20200223
PL  - England
TA  - J Fish Biol
JT  - Journal of fish biology
JID - 0214055
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Fish Proteins)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
EIN - J Fish Biol. 2020 Jul;97(1):318-319. PMID: 32681696
MH  - Adjuvants, Immunologic/pharmacology
MH  - Animals
MH  - Energy Metabolism/*drug effects
MH  - Fish Proteins/genetics
MH  - Gene Expression Regulation/*drug effects
MH  - Head Kidney/drug effects
MH  - Immune System/*drug effects
MH  - Leukocytes/drug effects
MH  - Phagocytosis/drug effects
MH  - Poly I-C/*pharmacology
MH  - Salmon/*immunology/metabolism
OTO - NOTNLM
OT  - Chinook salmon
OT  - cytokine transcript expression
OT  - fish immunology
OT  - metabolomics
OT  - peripheral blood mononuclear cells
OT  - poly (I:C)
EDAT- 2020/01/30 06:00
MHDA- 2020/06/26 06:00
CRDT- 2020/01/30 06:00
PHST- 2019/10/29 00:00 [received]
PHST- 2020/01/27 00:00 [accepted]
PHST- 2020/01/30 06:00 [pubmed]
PHST- 2020/06/26 06:00 [medline]
PHST- 2020/01/30 06:00 [entrez]
AID - 10.1111/jfb.14266 [doi]
PST - ppublish
SO  - J Fish Biol. 2020 Mar;96(3):731-746. doi: 10.1111/jfb.14266. Epub 2020 Feb 23.