PMID- 31997722
OWN - NLM
STAT- MEDLINE
DCOM- 20210812
LR  - 20221207
IS  - 1479-487X (Electronic)
IS  - 0959-3330 (Linking)
VI  - 42
IP  - 20
DP  - 2021 Aug
TI  - In-depth assessment of microbial communities in the full-scale vertical flow 
      treatment wetlands fed with raw domestic wastewater.
PG  - 3106-3121
LID - 10.1080/09593330.2020.1723709 [doi]
AB  - A multiphase study was proposed to examine microbial communities linked to the 
      nitrogen cycle in the first stage of four full-scale French vertical flow 
      treatment systems. To this end, denaturing gradient gel electrophoresis (DGGE) 
      was performed for structural assessment and quantitative PCR (qPCR) to enumerate 
      the abundance of ammonia-oxidizing (AOB). 16S rRNA sequencing was used to assess 
      the taxonomic profile followed by putative assessment of functional genes. The 
      samples were collected under different conditions, such as operational time 
      (presence/absence of sludge layer on the surface of the filters), season (winter 
      and summer), sampling depth (0, 15 and 30 cm) and operation cycle (rest and feed 
      periods). A structural disparity was noted in the upper layers, whereas higher 
      similarity at 30 cm was observed highlighting the effect of organic matter on 
      bacterial diversity. The 7th rest day was highlighted by an apparent decline in 
      the microbial community abundance. Additionally, qPCR indicated that the largest 
      amount of AOB was found at 30 cm depth and during the feeding period. From the 
      taxonomic profile, Mycobacterium, Acinetobacter, Flavobacterium, and Nitrospira 
      were the most abundant genre found in all systems. The functional prediction 
      results showed predicted genes linked to the denitrification process. The results 
      suggested that operating time and season were responsible for the pattern of the 
      microbial community behavior. This study allowed us to further understand the 
      bacterial dynamics and to advance the idea of design modifications made in the 
      first stage of the classical French system to improve nitrogen removal are 
      promising.
FAU - Silveira, D D
AU  - Silveira DD
AUID- ORCID: 0000-0002-8299-0030
AD  - UFSC, Federal University of Santa Catarina, Florianopolis, Brazil.
AD  - INRAE, Villeurbanne, France.
FAU - Filho, P Belli
AU  - Filho PB
AD  - UFSC, Federal University of Santa Catarina, Florianopolis, Brazil.
FAU - Philippi, L S
AU  - Philippi LS
AD  - UFSC, Federal University of Santa Catarina, Florianopolis, Brazil.
FAU - Cantao, M E
AU  - Cantao ME
AD  - EMBRAPA SUINOS E AVES, Concordia, Brazil.
FAU - Foulquier, A
AU  - Foulquier A
AD  - CNRS, LECA, Univ. Grenoble Alpes, Univ. Savoie Mont Blanc Grenoble, France.
FAU - Bayle, S
AU  - Bayle S
AD  - LGEI, IMT Mines Ales, Univ. Montpellier, Ales, France.
FAU - Delforno, T P
AU  - Delforno TP
AUID- ORCID: 0000-0002-1705-0763
AD  - Microbial Resources Division, Research Center for Chemistry, Biology and 
      Agriculture (CPQBA), Campinas University - UNICAMP, Campinas, Brazil.
FAU - Molle, P
AU  - Molle P
AD  - INRAE, Villeurbanne, France.
LA  - eng
PT  - Journal Article
DEP - 20200209
PL  - England
TA  - Environ Technol
JT  - Environmental technology
JID - 9884939
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Waste Water)
RN  - 7664-41-7 (Ammonia)
RN  - N762921K75 (Nitrogen)
SB  - IM
MH  - Ammonia
MH  - *Microbiota/genetics
MH  - Nitrogen
MH  - RNA, Ribosomal, 16S/genetics
MH  - Wastewater
MH  - *Wetlands
OTO - NOTNLM
OT  - French VF wetland
OT  - Functional prediction
OT  - Microbial community structure
OT  - Nitrifying bacteria
OT  - Taxonomic profile
EDAT- 2020/01/31 06:00
MHDA- 2021/08/13 06:00
CRDT- 2020/01/31 06:00
PHST- 2020/01/31 06:00 [pubmed]
PHST- 2021/08/13 06:00 [medline]
PHST- 2020/01/31 06:00 [entrez]
AID - 10.1080/09593330.2020.1723709 [doi]
PST - ppublish
SO  - Environ Technol. 2021 Aug;42(20):3106-3121. doi: 10.1080/09593330.2020.1723709. 
      Epub 2020 Feb 9.