PMID- 31998896
OWN - NLM
STAT- MEDLINE
DCOM- 20201123
LR  - 20201123
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 11
IP  - 2
DP  - 2020 Feb 26
TI  - Grape pomace extract supplementation activates FNDC5/irisin in muscle and 
      promotes white adipose browning in rats fed a high-fat diet.
PG  - 1537-1546
LID - 10.1039/c9fo02463h [doi]
AB  - Irisin is a myokine regulated by peroxisome proliferator-activated receptor gamma 
      co-activator-1alpha (PGC-1alpha) in the exercising skeletal muscle and released into the 
      bloodstream after cleavage of FNDC5. Circulating irisin can up-regulate UCP-1 
      expression in white adipose tissue (WAT) promoting the formation of brown-like 
      adipocytes. The aim of this study was to evaluate if supplementation with a grape 
      pomace extract (GPE) could activate the FNDC5/irisin pathway via PGC-1alpha in rats 
      fed a high fat diet (HFD). For this purpose we characterized the activation of: 
      i. the FNDC5/irisin pathway and AMPK in skeletal muscle and ii. proteins involved 
      in the formation of brown-like cells in epididymal WAT (eWAT). Consumption of the 
      GPE activated the FNDC5/irisin pathway, increased AMPK phosphorylation in 
      skeletal muscle and enhanced irisin plasma levels. In eWAT, the GPE increased the 
      level of proteins involved in WAT browning, i.e. PGC-1alpha, PPARgamma, PRDM16 and UCP-1. 
      The GPE also prevented HFD-induced adipocyte hypertrophy and systemic insulin 
      resistance. Consistently, in L6 myotubes, (-)-epicatechin (EC), a flavonoid 
      abundant in the GPE, prevented palmitate-mediated downregulation of FNDC5/irisin 
      protein expression and secretion, in part via PGC-1alpha activation. Consumption of 
      the GPE, a winemaking residue rich in bioactive compounds, could be a beneficial 
      strategy to counteract the adverse effects of Western style diets through the 
      promotion of WAT browning.
FAU - Rodriguez Lanzi, Cecilia
AU  - Rodriguez Lanzi C
AD  - Laboratorio de Nutricion y Fisiopatologia de la Obesidad, Instituto de Medicina y 
      Biologia Experimental de Cuyo (IMBECU)-CONICET, Mendoza, Argentina. 
      mvazquez@fcm.uncu.edu.ar.
FAU - Perdicaro, Diahann J
AU  - Perdicaro DJ
FAU - Gambarte Tudela, Julian
AU  - Gambarte Tudela J
FAU - Muscia, Victoria
AU  - Muscia V
FAU - Fontana, Ariel R
AU  - Fontana AR
FAU - Oteiza, Patricia I
AU  - Oteiza PI
FAU - Vazquez Prieto, Marcela A
AU  - Vazquez Prieto MA
LA  - eng
PT  - Journal Article
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 0 (FNDC5 protein, rat)
RN  - 0 (Fibronectins)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/drug effects/metabolism
MH  - Adipose Tissue, Brown/drug effects/metabolism
MH  - Adipose Tissue, White/*drug effects/metabolism
MH  - Animals
MH  - Diet, High-Fat
MH  - Fibronectins/*metabolism
MH  - Male
MH  - Mice
MH  - Muscle, Skeletal/*drug effects/metabolism
MH  - Plant Extracts/*pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Vitis
EDAT- 2020/01/31 06:00
MHDA- 2020/11/24 06:00
CRDT- 2020/01/31 06:00
PHST- 2020/01/31 06:00 [pubmed]
PHST- 2020/11/24 06:00 [medline]
PHST- 2020/01/31 06:00 [entrez]
AID - 10.1039/c9fo02463h [doi]
PST - ppublish
SO  - Food Funct. 2020 Feb 26;11(2):1537-1546. doi: 10.1039/c9fo02463h.