PMID- 32000560
OWN - NLM
STAT- MEDLINE
DCOM- 20201207
LR  - 20201214
IS  - 1532-4281 (Electronic)
IS  - 1079-9893 (Linking)
VI  - 40
IP  - 2
DP  - 2020 Apr
TI  - Cabin1 involves in renal tubular epithelial cells mitochondrial dysfunction 
      through SIRT1/p53 pathway.
PG  - 141-147
LID - 10.1080/10799893.2020.1719518 [doi]
AB  - Background: Angiotensin II (AngII) induced Calcineurin binding protein 1 (Cabin1) 
      protein expression significantly increased during Renal tubular epithelial cells 
      (RTEC) injury. However, the detailed function of Cabin1 protein in RTEC was not 
      characterized well. In this study, we aimed to explore the downstream target of 
      Cabin1 in vitro model.Methods: Rat kidney epithelial cells were cultured and 
      stimulated with AngII. Electron microscopy was performed to observe mitochondrial 
      morphology change. Immunofluorescence staining was detected to observe the 
      distribution of cytoskeleton and Cabin1. Mitochondrial morphology change and 
      protein expression were detected by electrical microscopy and western 
      blot.Results: AngII induced the disruption of cytoskeleton at 24 and 48 h. 
      Western blot analysis showed AngII significantly induced the overexpression of 
      Cabin1. AngII induced a great deal of small, long and irregular mitochondria in 
      RTEC, aspect ratio which reflects the length-to-width ratio of mitochondria 
      remarkably increased at 12 and 24 h. Knocking down Cabin1 aggravated 
      mitochondrial morphological abnormality in AngII treated RTEC. In comparison with 
      control, Cabin1, p53 and cyto C level were significantly increased in AngII 
      treated cells, while SIRT1 level was obviously decreased. Knocked down Cabin1 
      plus AngII stimulated, SIRT1 was further decreased, while p53 and cyto C were 
      significantly increased.Conclusions: Cabin1 involves in RTEC mitochondrial 
      dysfunction through SIRT1/p53 pathway. Cabin1 may be used as a new marker for the 
      mechanisms of RTEC injury.
FAU - Wen, Yueqiang
AU  - Wen Y
AD  - Department of Nephrology, The Second Affiliated Hospital, Guangzhou Medical 
      University, Guangzhou, China.
FAU - Chen, Jieru
AU  - Chen J
AD  - Department of Intensive Care Unit, The Second Affiliated Hospital, Guangzhou 
      Medical University, Guangzhou, China.
FAU - Liu, Lingling
AU  - Liu L
AD  - Department of General Medicine, The Third Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, China.
FAU - Guo, Guanhua
AU  - Guo G
AD  - Department of Nephrology, The Second Affiliated Hospital, Guangzhou Medical 
      University, Guangzhou, China.
FAU - Zeng, Yinsi
AU  - Zeng Y
AD  - Department of Nephrology, The Second Affiliated Hospital, Guangzhou Medical 
      University, Guangzhou, China.
FAU - Zhang, Yujing
AU  - Zhang Y
AD  - Department of Nephrology, The Second Affiliated Hospital, Guangzhou Medical 
      University, Guangzhou, China.
FAU - Zeng, Zhili
AU  - Zeng Z
AD  - Department of Nephrology, The Second Affiliated Hospital, Guangzhou Medical 
      University, Guangzhou, China.
FAU - Wang, Zebin
AU  - Wang Z
AD  - Department of Nephrology, The Second Affiliated Hospital, Guangzhou Medical 
      University, Guangzhou, China.
FAU - Peng, Xuan
AU  - Peng X
AD  - Department of Intensive Care Unit, The Second Affiliated Hospital, Guangzhou 
      Medical University, Guangzhou, China.
FAU - Liang, Jianbo
AU  - Liang J
AD  - Department of Nephrology, The Second Affiliated Hospital, Guangzhou Medical 
      University, Guangzhou, China.
LA  - eng
PT  - Journal Article
DEP - 20200131
PL  - England
TA  - J Recept Signal Transduct Res
JT  - Journal of receptor and signal transduction research
JID - 9509432
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Cabin1 protein, rat)
RN  - 0 (Tp53 protein, rat)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 11128-99-7 (Angiotensin II)
RN  - EC 3.5.1.- (Sirt1 protein, rat)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - Angiotensin II/*genetics/pharmacology
MH  - Animals
MH  - Apoptosis Regulatory Proteins/*genetics
MH  - Epithelial Cells/metabolism/pathology
MH  - Humans
MH  - Kidney Tubules/injuries/metabolism/pathology
MH  - Male
MH  - Mitochondria/*genetics/metabolism/pathology
MH  - Nephrectomy
MH  - Podocytes/metabolism/pathology
MH  - Rats
MH  - Sirtuin 1/*genetics
MH  - Tumor Suppressor Protein p53/*genetics
OTO - NOTNLM
OT  - Cabin1
OT  - SIRT1
OT  - mitochondrial dysfunction
OT  - p53
OT  - renal tubular epithelial cells
EDAT- 2020/02/01 06:00
MHDA- 2020/12/15 06:00
CRDT- 2020/02/01 06:00
PHST- 2020/02/01 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/02/01 06:00 [entrez]
AID - 10.1080/10799893.2020.1719518 [doi]
PST - ppublish
SO  - J Recept Signal Transduct Res. 2020 Apr;40(2):141-147. doi: 
      10.1080/10799893.2020.1719518. Epub 2020 Jan 31.