PMID- 32000907
OWN - NLM
STAT- MEDLINE
DCOM- 20201218
LR  - 20201218
IS  - 1557-7988 (Electronic)
IS  - 0076-6879 (Linking)
VI  - 632
DP  - 2020
TI  - Cytofluorometric assessment of dendritic cell-mediated uptake of cancer cell 
      apoptotic bodies.
PG  - 39-54
LID - S0076-6879(19)30350-7 [pii]
LID - 10.1016/bs.mie.2019.08.005 [doi]
AB  - Dendritic cells (DCs) are specialized antigen presenting cells (APCs) able to 
      intake and crosspresent antigens (Ags) on major histocompatibility complex (MHC) 
      class I and II molecules to T cells thus initiating primary and memory immune 
      responses. DC-mediated Ag uptake and crosspresentation represent crucial steps 
      toward cancer recognition and eventually elimination. Cytofluorometry is a 
      standardized procedure to study phagocytosis. By fast and reproducible single 
      cell measurements, flow cytometry allows for simultaneous biochemical and 
      functional analyses of Ag intake. In this chapter, we discuss a two-color flow 
      cytometric analysis of DC-mediated uptake of apoptotic bodies. We also show data 
      on the adjuvanticity of Type-I-interferons (Type-I-IFNs) during Ag retention as 
      we offer a guideline and a range of advice on sample preparation and acquisition.
CI  - (c) 2020 Elsevier Inc. All rights reserved.
FAU - Musella, Martina
AU  - Musella M
AD  - Unit of Tumor Immunology and Immunotherapy, Department of Research, Advanced 
      Diagnostics, and Technological Innovation, IRCCS Regina Elena National Cancer 
      Institute, Rome, Italy; Department of Molecular Medicine, University "La 
      Sapienza", Rome, Italy.
FAU - Manic, Gwenola
AU  - Manic G
AD  - Unit of Tumor Immunology and Immunotherapy, Department of Research, Advanced 
      Diagnostics, and Technological Innovation, IRCCS Regina Elena National Cancer 
      Institute, Rome, Italy.
FAU - Galassi, Claudia
AU  - Galassi C
AD  - Istituto di Patologia Generale, Universita Cattolica del Sacro Cuore, Rome, 
      Italy.
FAU - Vitale, Ilio
AU  - Vitale I
AD  - Italian Institute for Genomic Medicine (IIGM), Turin, Italy; Candiolo Cancer 
      Institute-FPO, IRCCS, Candiolo, Italy. Electronic address: iliovit@gmail.com.
FAU - Sistigu, Antonella
AU  - Sistigu A
AD  - Unit of Tumor Immunology and Immunotherapy, Department of Research, Advanced 
      Diagnostics, and Technological Innovation, IRCCS Regina Elena National Cancer 
      Institute, Rome, Italy; Istituto di Patologia Generale, Universita Cattolica del 
      Sacro Cuore, Rome, Italy. Electronic address: antonella.sistigu@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20191018
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Coculture Techniques/methods
MH  - Dendritic Cells/*immunology
MH  - Extracellular Vesicles/*immunology
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - Immunogenic Cell Death
MH  - Mice
MH  - Neoplasms/*immunology
MH  - Phagocytosis
OTO - NOTNLM
OT  - Antigen presenting cells
OT  - Cytofluorometry
OT  - Immunogenic cell death
OT  - Phagocytosis
OT  - Tumor
EDAT- 2020/02/01 06:00
MHDA- 2020/12/19 06:00
CRDT- 2020/02/01 06:00
PHST- 2020/02/01 06:00 [entrez]
PHST- 2020/02/01 06:00 [pubmed]
PHST- 2020/12/19 06:00 [medline]
AID - S0076-6879(19)30350-7 [pii]
AID - 10.1016/bs.mie.2019.08.005 [doi]
PST - ppublish
SO  - Methods Enzymol. 2020;632:39-54. doi: 10.1016/bs.mie.2019.08.005. Epub 2019 Oct 
      18.