PMID- 32003502
OWN - NLM
STAT- MEDLINE
DCOM- 20200413
LR  - 20210110
IS  - 1365-2184 (Electronic)
IS  - 0960-7722 (Print)
IS  - 0960-7722 (Linking)
VI  - 53
IP  - 3
DP  - 2020 Mar
TI  - The 5.8S pre-rRNA maturation factor, M-phase phosphoprotein 6, is a female 
      fertility factor required for oocyte quality and meiosis.
PG  - e12769
LID - 10.1111/cpr.12769 [doi]
LID - e12769
AB  - OBJECTIVES: M-phase phosphoprotein 6 (MPP6) is important for 5.8S pre-rRNA 
      maturation in somatic cells and was screened as a female fertility factor. 
      However, whether MPP6 functions in oocyte meiosis and fertility is not yet known. 
      We aimed to address this. MATERIALS AND METHODS: Mouse oocytes with surrounded 
      nucleus (SN) or non-surrounded nucleus (NSN) were used for all experiments. 
      Peptide nanoparticle-mediated antibody transfection was used to deplete MPP6. 
      Immunofluorescence staining, immunohistochemistry and live tracker staining were 
      used to examine MPP6 localization and characterize phenotypes after control or 
      MPP6 depletion. High-fidelity PCR and fluorescence in situ hybridization (FISH) 
      were used to examine the localization and level of 5.8S rRNAs. Western blot was 
      used to examine the protein level. MPP6-EGFP mRNA microinjection was used to do 
      the rescue. RESULTS: MPP6 was enriched within ovaries and oocytes. MPP6 depletion 
      significantly impeded oocyte meiosis. MPP6 depletion increased 5.8S pre-rRNA. The 
      mRNA levels of MPP6 and 5.8S rRNA decreased within ageing oocytes, and MPP6 mRNA 
      injection partially increased 5.8S rRNA maturation and improved oocyte quality. 
      CONCLUSIONS: MPP6 is required for 5.8S rRNA maturation, meiosis and quality 
      control in mouse oocytes, and MPP6 level might be a marker for oocyte quality.
CI  - (c) 2020 The Authors. Cell Proliferation published by John Wiley & Sons Ltd.
FAU - Peng, Rui-Rui
AU  - Peng RR
AD  - Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to 
      Shandong University, Jinan, China.
AD  - State Key Lab of Reproductive Medicine, Nanjing Medical University, Nanjing, 
      China.
FAU - Wang, Li-Li
AU  - Wang LL
AD  - State Key Lab of Reproductive Medicine, Nanjing Medical University, Nanjing, 
      China.
FAU - Gao, Wen-Yi
AU  - Gao WY
AD  - State Key Lab of Reproductive Medicine, Nanjing Medical University, Nanjing, 
      China.
FAU - Zhu, Feng-Yu
AU  - Zhu FY
AD  - State Key Lab of Reproductive Medicine, Nanjing Medical University, Nanjing, 
      China.
FAU - Hu, Fan
AU  - Hu F
AD  - State Key Lab of Reproductive Medicine, Nanjing Medical University, Nanjing, 
      China.
FAU - Zeng, Wen-Tao
AU  - Zeng WT
AD  - Animal Core Facility, Nanjing Medical University, Nanjing, China.
FAU - Shi, Li-Ya
AU  - Shi LY
AD  - The Second Affiliated Hospital, Nanjing Medical University, Nanjing, China.
FAU - Chen, Xi-Chen
AU  - Chen XC
AD  - Analysis and Test Center, Nanjing Medical University, Nanjing, China.
FAU - Cai, Jing-Yang
AU  - Cai JY
AD  - State Key Lab of Reproductive Medicine, Nanjing Medical University, Nanjing, 
      China.
FAU - Zhang, Dong
AU  - Zhang D
AUID- ORCID: 0000-0002-0257-4441
AD  - State Key Lab of Reproductive Medicine, Nanjing Medical University, Nanjing, 
      China.
AD  - Animal Core Facility, Nanjing Medical University, Nanjing, China.
FAU - Xia, Zheng-Rong
AU  - Xia ZR
AD  - Analysis and Test Center, Nanjing Medical University, Nanjing, China.
FAU - Yang, Zhi-Xia
AU  - Yang ZX
AD  - State Key Lab of Reproductive Medicine, Nanjing Medical University, Nanjing, 
      China.
LA  - eng
GR  - 31671561/General Program of the National Natural Science Foundation of China/
GR  - NMUB2018044/Science and Technology Development Fund of Nanjing Medical 
      University/
GR  - 2017YFC1001503/National Key Research and Development Program of China/
GR  - 2017NJMUZD015/Science and Technology Development Fund of Nanjing Medical 
      University/
PT  - Journal Article
DEP - 20200131
PL  - England
TA  - Cell Prolif
JT  - Cell proliferation
JID - 9105195
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Mphosph6 protein, mouse)
RN  - 0 (RNA, Ribosomal, 5.8S)
RN  - 0 (RNA-Binding Proteins)
SB  - IM
MH  - Animals
MH  - Cell Cycle Proteins/genetics/*metabolism
MH  - Cell Division
MH  - Cells, Cultured
MH  - Cellular Senescence
MH  - Female
MH  - Fertility
MH  - Fertilization in Vitro
MH  - Male
MH  - Meiosis
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Oocytes/*cytology/metabolism/ultrastructure
MH  - RNA, Ribosomal, 5.8S/*metabolism
MH  - RNA-Binding Proteins/genetics/*metabolism
PMC - PMC7106954
OTO - NOTNLM
OT  - 5.8S Pre-rRNA
OT  - M-phase phosphoprotein 6
OT  - female fertility factor
OT  - meiosis
OT  - oocyte
OT  - quality
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2020/02/01 06:00
MHDA- 2020/04/14 06:00
PMCR- 2020/01/31
CRDT- 2020/02/01 06:00
PHST- 2019/09/07 00:00 [received]
PHST- 2019/12/07 00:00 [revised]
PHST- 2020/01/04 00:00 [accepted]
PHST- 2020/02/01 06:00 [pubmed]
PHST- 2020/04/14 06:00 [medline]
PHST- 2020/02/01 06:00 [entrez]
PHST- 2020/01/31 00:00 [pmc-release]
AID - CPR12769 [pii]
AID - 10.1111/cpr.12769 [doi]
PST - ppublish
SO  - Cell Prolif. 2020 Mar;53(3):e12769. doi: 10.1111/cpr.12769. Epub 2020 Jan 31.