PMID- 32003557
OWN - NLM
STAT- MEDLINE
DCOM- 20210119
LR  - 20210119
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 92
IP  - 5
DP  - 2020 Mar 3
TI  - Diamond-Like Carbon Thin Film Electrodes for Microfluidic Bioelectrochemical 
      Sensing Platforms.
PG  - 3650-3657
LID - 10.1021/acs.analchem.9b04689 [doi]
AB  - This work aims to utilize diamond-like carbon (DLC) thin films for bioreceptor 
      immobilization and amperometric biosensing in a microfluidic platform. A specific 
      RF-PECVD method was employed to prepare DLC thin film electrodes with desirable 
      surface and bulk properties. The films possessed a relatively high sp(2) 
      fraction, a moderate electrical conductivity (7.75 x 10(-3) S cm(-1)), and an 
      optical band gap of 1.67 eV. X-ray photoelectron spectroscopy (XPS) and 
      attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy 
      revealed a presence of oxygen-containing functional groups on the DLC surface. 
      The DLC electrodes were integrated into polydimethylsiloxane (PDMS) microfluidic 
      electrochemical cells with the channel volume of 2.24 muL. Glucose oxidase (GOx) 
      was chosen as a model bioreceptor to validate the employment of DLC electrodes 
      for bioelectrochemical sensing. In-channel immobilization of glucose oxidase 
      (GOx) at the DLC surface was realized through carbodiimide covalent linkages. 
      Enzyme bound DLC electrode was confirmed with the redox potential at around -79 
      mV vs NHE in 0.1 M phosphate buffer pH 7.4. Amperometric flow-injection glucose 
      sensing at a potential of -0.45 V vs Ag in the absence of standard redox 
      mediators showed the increase of current response upon increasing the glucose 
      concentration. The sensing mechanism is based on the reduction process of 
      H(2)O(2) liberated from the enzymatic activity. The proposed model for the 
      catalytic H(2)O(2) reduction to H(2)O on DLC electrodes was attributed to the 
      dissociation of C-O bonds at the DLC surface.
FAU - Triroj, Napat
AU  - Triroj N
AUID- ORCID: 0000-0003-3125-476X
AD  - Department of Electrical Engineering, Faculty of Engineering, Khon Kaen 
      University, Khon Kaen 40002, Thailand.
FAU - Saensak, Rattanakorn
AU  - Saensak R
AD  - Department of Electrical Engineering, Faculty of Engineering, Khon Kaen 
      University, Khon Kaen 40002, Thailand.
FAU - Porntheeraphat, Supanit
AU  - Porntheeraphat S
AD  - Thai Microelectronics Center (TMEC), Chachoengsao 24000, Thailand.
AD  - National Electronics and Computer Technology Center, Pathum Thani 12120, 
      Thailand.
FAU - Paosawatyanyong, Boonchoat
AU  - Paosawatyanyong B
AD  - Department of Physics, Faculty of Science, Chulalongkorn University, Bangkok 
      10330, Thailand.
FAU - Amornkitbamrung, Vittaya
AU  - Amornkitbamrung V
AD  - Department of Physics, Faculty of Science, Khon Kaen University, Khon Kaen 40002, 
      Thailand.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200214
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 7782-40-3 (Diamond)
SB  - IM
MH  - Diamond/*chemistry
MH  - Electrochemistry/*instrumentation
MH  - Electrodes
MH  - Equipment Design
MH  - *Lab-On-A-Chip Devices
EDAT- 2020/02/01 06:00
MHDA- 2021/01/20 06:00
CRDT- 2020/02/01 06:00
PHST- 2020/02/01 06:00 [pubmed]
PHST- 2021/01/20 06:00 [medline]
PHST- 2020/02/01 06:00 [entrez]
AID - 10.1021/acs.analchem.9b04689 [doi]
PST - ppublish
SO  - Anal Chem. 2020 Mar 3;92(5):3650-3657. doi: 10.1021/acs.analchem.9b04689. Epub 
      2020 Feb 14.