PMID- 32003673
OWN - NLM
STAT- MEDLINE
DCOM- 20210723
LR  - 20210723
IS  - 1875-5739 (Electronic)
IS  - 1567-2026 (Linking)
VI  - 17
IP  - 1
DP  - 2020
TI  - Forkhead Box O1-p21 Mediates Macrophage Polarization in Postoperative Cognitive 
      Dysfunction Induced by Sevoflurane.
PG  - 79-85
LID - 10.2174/1567202617666200128142728 [doi]
AB  - PURPOSE: The current study was conducted in order to investigate the role of 
      Forkhead box O1 and p21-mediated macrophage polarization in postoperative 
      cognitive dysfunction induced by sevoflurane. METHODS: There involved a total of 
      30 healthy mice that were randomly divided into two groups: control group 
      (without any treatment) and anaesthesia group (treated with sevoflurane 
      inhalation). The effects of sevoflurane on cognitive function (memory) in mice 
      were studied by trace fear conditioned reflex, and the effects of systemic 
      inflammation and behavior after operation were measured by enzyme-linked 
      immunosorbent assay (ELISA), the concentrations of CD163 and tumor necrosis 
      factor-alpha (TNF-alpha) were measured. The expression of macrophage phenotype was 
      observed by immunofluorescence staining, the expression levels of M1 and M2 
      markers mRNA were detected by real-time fluorescence quantitative PCR (RT-PCR), 
      and the expression levels of FoxO1 and p21 were analyzed by immunoblotting 
      (Western blot). RESULTS: Compared with the control group, the freezing time in 
      the anesthesia group was lower than that in the control group (P<0.01), 
      indicating that sevoflurane anesthesia led to the decrease of cognitive ability. 
      The blood concentrations of CD163 and TNF-alpha increased significantly at 24 h after 
      the operation with sevoflurane anesthesia (P<0.05). Fluorescence microscopic 
      observation showed that M2 was the main type of macrophages in normal tissues, 
      while M1 and M2 phenotypes were highly expressed in sevoflurane anesthetized 
      tissues at the same time, especially in M1 phenotypes (P<0.01). The polarization 
      of macrophages in the anesthetic group showed the high level of M1 mRNA, and the 
      expression levels of TNF-alpha, monocyte chemotactic protein 1(MCP-1) and 
      Interleukin-6 (IL-6)mRNA in the anesthetic group were significantly higher than 
      those in the control group (P<0.05). The expression levels of M2 mRNA such as 
      transforming growth factor-beta (TGF-beta) and IL-10 were significantly lower than 
      those in the control group (P<0.05). Compared with the control group, the 
      expression of FoxO1 and p21 protein in the anesthesia group was significantly 
      lower than that in the control group with a significant statistical difference 
      (P<0.01). CONCLUSION: This study offers a theoretical basis and insight for 
      further understanding of the prevention and treatment of cognitive dysfunction 
      induced by anesthetic drugs.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - Fu, Jun-Bao
AU  - Fu JB
AD  - Department of Anesthesiology, Linyi Central Hospital, Linyi, Shandong, China.
FAU - Wang, Zhi-Hua
AU  - Wang ZH
AD  - Department of Anesthesiology, Linyi Central Hospital, Linyi, Shandong, China.
FAU - Ren, Yong-Ying
AU  - Ren YY
AD  - Department of Anesthesiology, Linyi Central Hospital, Linyi, Shandong, China.
LA  - eng
PT  - Journal Article
PL  - United Arab Emirates
TA  - Curr Neurovasc Res
JT  - Current neurovascular research
JID - 101208439
RN  - 0 (Anesthetics, Inhalation)
RN  - 0 (Cytokines)
RN  - 0 (Forkhead Box Protein O1)
RN  - 0 (Foxo1 protein, mouse)
RN  - 38LVP0K73A (Sevoflurane)
SB  - IM
MH  - Anesthetics, Inhalation/*adverse effects
MH  - Animals
MH  - Cell Polarity/*physiology
MH  - Cytokines/metabolism
MH  - Forkhead Box Protein O1/*metabolism
MH  - Hippocampus/metabolism
MH  - Macrophage Activation
MH  - Macrophages/*metabolism
MH  - Male
MH  - Mice
MH  - Postoperative Cognitive Complications/chemically induced/*metabolism
MH  - Sevoflurane/*adverse effects
OTO - NOTNLM
OT  - FoxO1
OT  - cognitive dysfunction
OT  - macrophage polarization
OT  - p21
OT  - sevoflurane
OT  - ventilation volume.
EDAT- 2020/02/01 06:00
MHDA- 2021/07/24 06:00
CRDT- 2020/02/01 06:00
PHST- 2019/11/11 00:00 [received]
PHST- 2019/11/21 00:00 [revised]
PHST- 2019/11/26 00:00 [accepted]
PHST- 2020/02/01 06:00 [pubmed]
PHST- 2021/07/24 06:00 [medline]
PHST- 2020/02/01 06:00 [entrez]
AID - CNR-EPUB-103990 [pii]
AID - 10.2174/1567202617666200128142728 [doi]
PST - ppublish
SO  - Curr Neurovasc Res. 2020;17(1):79-85. doi: 10.2174/1567202617666200128142728.