PMID- 32004817
OWN - NLM
STAT- MEDLINE
DCOM- 20210601
LR  - 20210601
IS  - 1532-1991 (Electronic)
IS  - 0143-4160 (Linking)
VI  - 87
DP  - 2020 May
TI  - Visible light excited ratiometric-GECIs for long-term in-cellulo monitoring of 
      calcium signals.
PG  - 102165
LID - S0143-4160(20)30007-5 [pii]
LID - 10.1016/j.ceca.2020.102165 [doi]
AB  - Genetically Encoded Calcium Indicators (GECIs) are powerful molecular tools for 
      monitoring calcium (Ca(2+)) signaling in the cytosol and organellar compartments. 
      However, currently available ratiometric indicators that allow measurements of 
      resting Ca(2+) levels have limitations in long-term Ca(2+) imaging. They either 
      are ultraviolet (UV)-excited ones with strong photo-toxicity, or have poor 
      performance. To overcome this hurdle, we developed a set of visible light excited 
      ratiometric-GECIs (VR-GECIs) based on existing mono-colored GECIs. With 
      performance comparable to their corresponding mono-color prototypes, this set of 
      VR-GECIs enables long-term measurements of intra-cellular or intra-organellar 
      Ca(2+) signals. Using these VR-GECIs together with a newly developed off-line 
      analysis tool, we achieved long-term measurements of Ca(2+) homeostasis of moving 
      or dividing cells. Our tools may find broad applications in decoding 
      Ca(2+)-modulated physiological or pathological processes.
CI  - Copyright (c) 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.
FAU - Li, Jia
AU  - Li J
AD  - Beijing Key Laboratory of Gene Resource and Molecular Development, College of 
      Life Sciences, Beijing Normal University, Beijing, 100875, China.
FAU - Wang, Liuqing
AU  - Wang L
AD  - Beijing Key Laboratory of Gene Resource and Molecular Development, College of 
      Life Sciences, Beijing Normal University, Beijing, 100875, China.
FAU - Chen, Yilan
AU  - Chen Y
AD  - Beijing Key Laboratory of Gene Resource and Molecular Development, College of 
      Life Sciences, Beijing Normal University, Beijing, 100875, China.
FAU - Yang, Yang
AU  - Yang Y
AD  - School of Civil Engineering and Transportation, South China University of 
      Technology, Guangdong, 510000, China.
FAU - Liu, Jin
AU  - Liu J
AD  - Beijing Key Laboratory of Gene Resource and Molecular Development, College of 
      Life Sciences, Beijing Normal University, Beijing, 100875, China.
FAU - Liu, Kai
AU  - Liu K
AD  - Beijing Key Laboratory of Gene Resource and Molecular Development, College of 
      Life Sciences, Beijing Normal University, Beijing, 100875, China.
FAU - Lee, Yi-Tsang
AU  - Lee YT
AD  - Institute of Biosciences and Technology, Texas A&M University, Houston, TX, 
      77030, USA.
FAU - He, Ningjia
AU  - He N
AD  - State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, 
      Chongqing, 400715, China.
FAU - Zhou, Yubin
AU  - Zhou Y
AD  - Institute of Biosciences and Technology, Texas A&M University, Houston, TX, 
      77030, USA.
FAU - Wang, Youjun
AU  - Wang Y
AD  - Beijing Key Laboratory of Gene Resource and Molecular Development, College of 
      Life Sciences, Beijing Normal University, Beijing, 100875, China; Key Laboratory 
      of Cell Proliferation and Regulation Biology, Ministry of Education, Institute of 
      Cell Biology, College of Life Sciences, Beijing Normal University, Beijing, 
      100875, China. Electronic address: wyoujun@bnu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200123
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Calcium/*metabolism
MH  - Calcium Signaling/*radiation effects
MH  - Cell Shape
MH  - Endoplasmic Reticulum/metabolism
MH  - Fluorescence
MH  - HEK293 Cells
MH  - HeLa Cells
MH  - Humans
MH  - *Light
MH  - Mitosis
OTO - NOTNLM
OT  - Calcium
OT  - ER
OT  - GECI
OT  - Homeostasis
OT  - Long-term
OT  - Mitochondria
OT  - Mitosis
OT  - Ratiometric
EDAT- 2020/02/01 06:00
MHDA- 2021/06/02 06:00
CRDT- 2020/02/01 06:00
PHST- 2019/12/09 00:00 [received]
PHST- 2020/01/21 00:00 [revised]
PHST- 2020/01/21 00:00 [accepted]
PHST- 2020/02/01 06:00 [pubmed]
PHST- 2021/06/02 06:00 [medline]
PHST- 2020/02/01 06:00 [entrez]
AID - S0143-4160(20)30007-5 [pii]
AID - 10.1016/j.ceca.2020.102165 [doi]
PST - ppublish
SO  - Cell Calcium. 2020 May;87:102165. doi: 10.1016/j.ceca.2020.102165. Epub 2020 Jan 
      23.