PMID- 32007417
OWN - NLM
STAT- MEDLINE
DCOM- 20200722
LR  - 20200722
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 22
IP  - 2
DP  - 2020 Feb
TI  - Effective naked plasmid DNA delivery into stem cells by microextrusion-based 
      transient-transfection system for in situ cardiac repair.
PG  - 70-81
LID - S1465-3249(19)30917-X [pii]
LID - 10.1016/j.jcyt.2019.12.003 [doi]
AB  - BACKGROUND AIMS: Combining the use of transfection reagents and physical methods 
      can markedly improve the efficiency of gene delivery; however, such methods often 
      cause cell damage. Additionally, naked plasmids without any vector or physical 
      stimulation are difficult to deliver into stem cells. In this study, we 
      demonstrate a simple and rapid method to simultaneously facilitate efficient in 
      situ naked gene delivery and form a bioactive hydrogel scaffold. METHODS: 
      Transfecting naked GATA binding protein 4 (GATA4) plasmids into human umbilical 
      cord-derived mesenchymal stem cells (hUC-MSCs) by co-extruding naked plasmids and 
      hUC-MSCs with a biomimetic and negatively charged water-based biodegradable 
      thermo-responsive polyurethane (PU) hydrogel through a microextrusion-based 
      transient-transfection system can upregulate the other cardiac marker genes. 
      RESULTS: The PU hydrogels with optimized physicochemical properties (such as 
      hard-soft segment composition, size, hardness and thermal gelation) induced 
      GATA4-transfected hUC-MSCs to express the cardiac marker proteins and then 
      differentiated into cardiomyocyte-like cells in 15 days. We further demonstrated 
      that GATA4-transfected hUC-MSCs in PU hydrogel were capable of in situ revival of 
      heart function in zebrafish in 30 days. CONCLUSIONS: Our results suggest that 
      hUC-MSCs and naked plasmids encapsulated in PU hydrogels might represent a new 
      strategy for in situ tissue therapy using the microextrusion-based 
      transient-transfection system described here. This transfection system is simple, 
      effective and safer than conventional technologies.
CI  - Copyright (c) 2019 International Society for Cell and Gene Therapy. Published by 
      Elsevier Inc. All rights reserved.
FAU - Huang, Nien-Chi
AU  - Huang NC
AD  - Institute of Polymer Science and Engineering, National Taiwan University, Taipei, 
      Taiwan, R.O.C.
FAU - Lee, Chii-Ming
AU  - Lee CM
AD  - Division of Cardiology, Department of Internal Medicine, National Taiwan 
      University Hospital, Taipei, Taiwan, R.O.C.
FAU - Hsu, Shan-Hui
AU  - Hsu SH
AD  - Institute of Polymer Science and Engineering, National Taiwan University, Taipei, 
      Taiwan, R.O.C.; Center of Tissue Engineering and 3D Printing, National Taiwan 
      University, Taipei, Taiwan, R.O.C.; Institute of Cellular and System Medicine, 
      National Health Research Institutes, Zhunan, Taiwan, R.O.C.. Electronic address: 
      shhsu@ntu.edu.tw.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200130
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - 0 (GATA4 Transcription Factor)
RN  - 0 (GATA4 protein, human)
RN  - 0 (Hydrogels)
RN  - 0 (Polyurethanes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cell- and Tissue-Based Therapy/*methods
MH  - Cellular Reprogramming/*genetics
MH  - DNA/genetics/metabolism
MH  - GATA4 Transcription Factor/*genetics
MH  - Genetic Therapy/methods
MH  - Heart/growth & development
MH  - Hydrogels/pharmacology
MH  - Mesenchymal Stem Cells/*cytology
MH  - Myocytes, Cardiac/*cytology
MH  - Plasmids/genetics
MH  - Polyurethanes/pharmacology
MH  - Transfection
MH  - Umbilical Cord/cytology
MH  - Zebrafish
OTO - NOTNLM
OT  - cardiac repair
OT  - cell reprogramming
OT  - in situ transfection
OT  - naked plasmid delivery
OT  - polyurethane hydrogels
EDAT- 2020/02/03 06:00
MHDA- 2020/07/23 06:00
CRDT- 2020/02/03 06:00
PHST- 2019/08/28 00:00 [received]
PHST- 2019/12/02 00:00 [revised]
PHST- 2019/12/03 00:00 [accepted]
PHST- 2020/02/03 06:00 [pubmed]
PHST- 2020/07/23 06:00 [medline]
PHST- 2020/02/03 06:00 [entrez]
AID - S1465-3249(19)30917-X [pii]
AID - 10.1016/j.jcyt.2019.12.003 [doi]
PST - ppublish
SO  - Cytotherapy. 2020 Feb;22(2):70-81. doi: 10.1016/j.jcyt.2019.12.003. Epub 2020 Jan 
      30.