PMID- 32007633 OWN - NLM STAT- MEDLINE DCOM- 20210127 LR - 20210127 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 256 DP - 2020 Jun 28 TI - Erchen decoction plus huiyanzhuyu decoction inhibits the cell cycle, migration and invasion and induces the apoptosis of laryngeal squamous cell carcinoma cells. PG - 112638 LID - S0378-8741(19)34385-5 [pii] LID - 10.1016/j.jep.2020.112638 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Laryngeal carcinoma (LC) is one of the most common malignant head and neck cancers with high incidence and mortality rates. Erchen decoction plus Huiyanzhuyu decoction (EHD) is commonly used for treating LC patients and produces beneficial results. However, the mechanisms underlying the effects of EHD remain unclear. AIM OF THE STUDY: The present study aimed to analyse the anticancer effects of EHD on the LC cell cycle, apoptosis, migration and invasion in vitro and to explore the underlying biological mechanisms. MATERIALS AND METHODS: TU212 and Hep-2 cells were used. The antitumour effects of EHD were detected by CCK8, microscopy, flow cytometry, EdU incorporation, Hoechst 33342 staining, wound-healing, and transwell assays to assess viability, morphology, apoptosis, cell cycle, migration and invasion, respectively. Furthermore, STAT3 and related proteins were evaluated in laryngeal squamous cell carcinoma (LSCC) cells by Western blot (WB) analysis. RESULTS: EHD treatment significantly decreased STAT3 and p-STAT3 protein expression levels in LSCC cells. EHD blocked the cell cycle at the G0/G1 phase and induced LSCC apoptosis. Moreover, the viability, migration, and invasion of LSCC cells were markedly inhibited by EHD. In addition, the expression of the cell cycle-related proteins cyclin D1 and cyclin B1 was downregulated in LSCC cells, but P27 expression was increased after EHD treatment. Regarding apoptosis-related proteins, EHD also reduced Bcl-2 expression but upregulated Bax and caspase-3 expression in LSCC cells. In the migration- and invasion-related protein analyses, EHD downregulated MMP-9 expression and upregulated E-cadherin expression. CONCLUSIONS: These results suggest that EHD has an anticancer effect in LSCC. EHD treatment induces apoptosis and inhibits the cell cycle, migration and invasion of LSCC cells, but further work is warranted to address the mechanisms. CI - Copyright (c) 2020. Published by Elsevier B.V. FAU - Zhou, Shiqing AU - Zhou S AD - Otorhinolaryngology Department, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China; Otorhinolaryngology Department, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510120, China. Electronic address: shiqingzhou@163.com. FAU - Luo, Qiulan AU - Luo Q AD - Otorhinolaryngology Department, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China; Otorhinolaryngology Department, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510120, China. Electronic address: doctorluoql77@gzucm.edu.cn. FAU - Tan, Xi AU - Tan X AD - Otorhinolaryngology Department, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China; Otorhinolaryngology Department, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510120, China. Electronic address: 1254429305@qq.com. FAU - Huang, Wei AU - Huang W AD - The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China. Electronic address: you.womanizer@qq.com. FAU - Feng, Xiaocong AU - Feng X AD - The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China. Electronic address: 2548470772@qq.com. FAU - Zhang, Tingting AU - Zhang T AD - Chinese Medicine Department, the Affiliated Hospital of Qingdao University, Qingdao, 266003, China. Electronic address: zhangtingting514@163.com. FAU - Chen, Wenyong AU - Chen W AD - Otorhinolaryngology Department, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China; Otorhinolaryngology Department, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510120, China. FAU - Yang, Chaojie AU - Yang C AD - Otorhinolaryngology Department, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China; Otorhinolaryngology Department, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510120, China. FAU - Li, Yunying AU - Li Y AD - Otorhinolaryngology Department, the Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510120, China; Otorhinolaryngology Department, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510120, China. Electronic address: docliyunying@gzucm.edu.cn. LA - eng PT - Journal Article DEP - 20200131 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Antineoplastic Agents) RN - 0 (Apoptosis Regulatory Proteins) RN - 0 (Cell Cycle Proteins) RN - 0 (Drugs, Chinese Herbal) SB - IM MH - Antineoplastic Agents/*therapeutic use MH - Apoptosis/*drug effects MH - Apoptosis Regulatory Proteins/metabolism MH - Cell Cycle/*drug effects MH - Cell Cycle Proteins/metabolism MH - Cell Line, Tumor MH - Cell Movement/*drug effects MH - Cell Proliferation/drug effects MH - Down-Regulation/drug effects MH - Drugs, Chinese Herbal/*therapeutic use MH - Gene Expression Regulation, Neoplastic/drug effects MH - Hep G2 Cells MH - Humans MH - Laryngeal Neoplasms/metabolism MH - Neoplasm Invasiveness/*prevention & control MH - Signal Transduction/drug effects MH - Squamous Cell Carcinoma of Head and Neck/*drug therapy/metabolism MH - Up-Regulation/drug effects OTO - NOTNLM OT - Chinese herbal medicine OT - Erchen decoction OT - Huiyanzhuyu decoction OT - Laryngeal squamous cell carcinoma cells OT - STAT3 COIS- Declaration of Competing interest The authors declare that there are no conflicts of interest regarding the publication of this paper. EDAT- 2020/02/03 06:00 MHDA- 2021/01/28 06:00 CRDT- 2020/02/03 06:00 PHST- 2019/11/02 00:00 [received] PHST- 2020/01/21 00:00 [revised] PHST- 2020/01/29 00:00 [accepted] PHST- 2020/02/03 06:00 [pubmed] PHST- 2021/01/28 06:00 [medline] PHST- 2020/02/03 06:00 [entrez] AID - S0378-8741(19)34385-5 [pii] AID - 10.1016/j.jep.2020.112638 [doi] PST - ppublish SO - J Ethnopharmacol. 2020 Jun 28;256:112638. doi: 10.1016/j.jep.2020.112638. Epub 2020 Jan 31.