PMID- 32008205
OWN - NLM
STAT- MEDLINE
DCOM- 20201211
LR  - 20201214
IS  - 1573-0832 (Electronic)
IS  - 0301-486X (Linking)
VI  - 185
IP  - 2
DP  - 2020 Apr
TI  - Fonsecaea pedrosoi Conidia Induces Activation of Dendritic Cells and Increases 
      CD11c(+) Cells in Regional Lymph Nodes During Experimental Chromoblastomycosis.
PG  - 245-256
LID - 10.1007/s11046-020-00429-w [doi]
AB  - The chromoblastomycosis is a subcutaneous mycosis with a high morbidity rate, 
      Fonsecaea pedrosoi being the largest etiologic agent of this mycosis, usually 
      confined to the skin and subcutaneous tissues. Rarely people get the cure, 
      because the therapies shown to be deficient and few studies report the 
      host-parasite relationship. Dendritic cells (DCs) are specialized in presenting 
      antigens to naive T lymphocytes inducing primary immune responses. Therefore, we 
      propose to study the migratory capacity of DCs after infection with conidia of F. 
      pedrosoi. The phenotype of DCs was evaluated using cells obtained from footpad 
      and lymph nodes of BALB/c mice after 12, 24 and 72 h of infection. After 24 and 
      72 h of infection, we found a significant decrease in DCs in footpad and a 
      significant increase in the lymph nodes after 72 h. The expression of surface 
      markers and co-stimulatory molecules were reduced in cells obtained from footpad. 
      To better assess the migratory capacity of DCs migration from footpad, 
      CFSE-stained conidia were injected subcutaneously. We found that after 12 and 
      72 h, CD11c(+) cells were increased in regional lymph nodes, leading us to 
      believe that DCs (CD11c(+)) were able to phagocytic conidia present in footpad 
      and migrated to regional lymph nodes.
FAU - Kimura, Telma Fatima Emidio
AU  - Kimura TFE
AD  - Laboratory of Mycology, Department of Clinical and Toxicological Analyses, 
      Faculty of Pharmaceutical Scienses, Universidade de Sao Paulo, Sao Paulo, Brazil.
FAU - Romera, Lavinia Maria Dal'Mas
AU  - Romera LMD
AUID- ORCID: 0000-0002-9625-0615
AD  - Laboratory of Mycology, Department of Clinical and Toxicological Analyses, 
      Faculty of Pharmaceutical Scienses, Universidade de Sao Paulo, Sao Paulo, Brazil. 
      laromera@live.com.
FAU - de Almeida, Sandro Rogerio
AU  - de Almeida SR
AD  - Laboratory of Mycology, Department of Clinical and Toxicological Analyses, 
      Faculty of Pharmaceutical Scienses, Universidade de Sao Paulo, Sao Paulo, Brazil.
LA  - eng
GR  - 10/03615-8/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
GR  - 10/03616-4/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
GR  - 16/04729-3/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
PT  - Journal Article
DEP - 20200201
PL  - Netherlands
TA  - Mycopathologia
JT  - Mycopathologia
JID - 7505689
RN  - 0 (CD11c Antigen)
RN  - Fonsecaea pedrosoi
SB  - IM
MH  - Animals
MH  - Ascomycota/immunology/pathogenicity
MH  - CD11c Antigen/metabolism
MH  - Cell Movement
MH  - Chromoblastomycosis/*immunology
MH  - Dendritic Cells/*metabolism
MH  - *Fonsecaea/immunology/pathogenicity
MH  - *Lymph Nodes
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Phagocytosis
MH  - Spores, Fungal/*immunology
OTO - NOTNLM
OT  - Cell migration
OT  - Chromoblastomycosis
OT  - Dendritic cells
OT  - Fonsecaea pedrosoi
EDAT- 2020/02/03 06:00
MHDA- 2020/12/15 06:00
CRDT- 2020/02/03 06:00
PHST- 2018/09/17 00:00 [received]
PHST- 2020/01/21 00:00 [accepted]
PHST- 2020/02/03 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/02/03 06:00 [entrez]
AID - 10.1007/s11046-020-00429-w [pii]
AID - 10.1007/s11046-020-00429-w [doi]
PST - ppublish
SO  - Mycopathologia. 2020 Apr;185(2):245-256. doi: 10.1007/s11046-020-00429-w. Epub 
      2020 Feb 1.