PMID- 32009222
OWN - NLM
STAT- MEDLINE
DCOM- 20201223
LR  - 20210302
IS  - 1573-7330 (Electronic)
IS  - 1058-0468 (Print)
IS  - 1058-0468 (Linking)
VI  - 37
IP  - 3
DP  - 2020 Mar
TI  - Karyotype evaluation of repeated abortions in primary and secondary recurrent 
      pregnancy loss.
PG  - 517-525
LID - 10.1007/s10815-020-01703-y [doi]
AB  - PURPOSE: To study the contribution of embryo chromosomal abnormalities in primary 
      and secondary recurrent pregnancy loss (RPL) and to analyze the recurrence of 
      chromosomal constitution in miscarriages from the same couple. METHODS: 
      Retrospective study of abortion karyotypes in RPL families based on the mother's 
      primary or secondary RPL status (563 embryo specimens, 335 samples from primary, 
      and 228 samples from secondary RPL). RPL was defined as two or more consecutive 
      miscarriages. One hundred eight cases of recurrent embryo/fetal loss in 51 
      families were analyzed to assess the probability of having the same karyotype 
      pattern (recurrent normal or recurrent abnormal) in both previous and subsequent 
      pregnancy loss. The karyotypes of abortions were established using standard 
      cytogenetic analysis, as well as interphase fluorescence in situ hybridization 
      (FISH) and comparative genomic hybridization (CGH). RESULTS: The frequency of 
      aberrations was 43.9% in abortions from primary RPL versus 52.6% in secondary RPL 
      (p = 0.041). Women 35 years of age or older were the main contributors to this 
      difference. The odds ratio of a subsequent abortion having the same karyotype 
      pattern (normal or abnormal) as the previous one was 6.98 (p = 0.0013). 
      CONCLUSION: The frequency of abnormalities is higher in abortions from the 
      secondary RPL versus primary RPL group, and this difference is due to the 
      relative deficiency of miscarriages with abnormal karyotypes in older women with 
      primary RPL. The probability of having the same karyotype pattern (recurrent 
      normal or recurrent abnormal) in the previous and subsequent abortion is 
      increased significantly compared with chance.
FAU - Nikitina, T V
AU  - Nikitina TV
AUID- ORCID: 0000-0002-4230-6855
AD  - Research Institute of Medical Genetics, Laboratory of Cytogenetics, Tomsk 
      National Research Medical Center of the Russian Academy of Sciences, Ushaika 
      Street 10, Tomsk, 634050, Russia. t.nikitina@medgenetics.ru.
FAU - Sazhenova, E A
AU  - Sazhenova EA
AUID- ORCID: 0000-0003-3875-3932
AD  - Research Institute of Medical Genetics, Laboratory of Cytogenetics, Tomsk 
      National Research Medical Center of the Russian Academy of Sciences, Ushaika 
      Street 10, Tomsk, 634050, Russia.
FAU - Zhigalina, D I
AU  - Zhigalina DI
AUID- ORCID: 0000-0003-2800-8875
AD  - Research Institute of Medical Genetics, Laboratory of Genomics of Orphan 
      Diseases, Tomsk National Research Medical Center of the Russian Academy of 
      Sciences, Ushaika Street 10, Tomsk, 634050, Russia.
FAU - Tolmacheva, E N
AU  - Tolmacheva EN
AUID- ORCID: 0000-0001-6427-3276
AD  - Research Institute of Medical Genetics, Laboratory of Cytogenetics, Tomsk 
      National Research Medical Center of the Russian Academy of Sciences, Ushaika 
      Street 10, Tomsk, 634050, Russia.
FAU - Sukhanova, N N
AU  - Sukhanova NN
AD  - Research Institute of Medical Genetics, Laboratory of Cytogenetics, Tomsk 
      National Research Medical Center of the Russian Academy of Sciences, Ushaika 
      Street 10, Tomsk, 634050, Russia.
FAU - Lebedev, I N
AU  - Lebedev IN
AUID- ORCID: 0000-0002-0482-8046
AD  - Research Institute of Medical Genetics, Laboratory of Cytogenetics, Tomsk 
      National Research Medical Center of the Russian Academy of Sciences, Ushaika 
      Street 10, Tomsk, 634050, Russia.
LA  - eng
GR  - AAAA-A19-11902089005-5/Ministry of Science and Education of the Russian 
      Federation/
PT  - Journal Article
DEP - 20200203
PL  - Netherlands
TA  - J Assist Reprod Genet
JT  - Journal of assisted reproduction and genetics
JID - 9206495
SB  - IM
MH  - Abortion, Induced/methods
MH  - Abortion, Spontaneous/diagnosis/*genetics/pathology
MH  - Adult
MH  - Aged
MH  - *Chromosome Aberrations
MH  - Comparative Genomic Hybridization
MH  - *Cytogenetic Analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Karyotype
MH  - Karyotyping/methods
MH  - Maternal Age
MH  - Pregnancy
PMC - PMC7125272
OTO - NOTNLM
OT  - Chromosomal abnormalities
OT  - Cytogenetics
OT  - Miscarriage
OT  - Primary and secondary recurrent pregnancy loss
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2020/02/06 06:00
MHDA- 2020/12/29 06:00
PMCR- 2021/03/01
CRDT- 2020/02/04 06:00
PHST- 2019/10/04 00:00 [received]
PHST- 2020/01/27 00:00 [accepted]
PHST- 2020/02/06 06:00 [pubmed]
PHST- 2020/12/29 06:00 [medline]
PHST- 2020/02/04 06:00 [entrez]
PHST- 2021/03/01 00:00 [pmc-release]
AID - 10.1007/s10815-020-01703-y [pii]
AID - 1703 [pii]
AID - 10.1007/s10815-020-01703-y [doi]
PST - ppublish
SO  - J Assist Reprod Genet. 2020 Mar;37(3):517-525. doi: 10.1007/s10815-020-01703-y. 
      Epub 2020 Feb 3.