PMID- 32010164
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 10
DP  - 2019
TI  - TaRPM1 Positively Regulates Wheat High-Temperature Seedling-Plant Resistance to 
      Puccinia striiformis f. sp. tritici.
PG  - 1679
LID - 10.3389/fpls.2019.01679 [doi]
LID - 1679
AB  - RPM1 is a CC-NBS-LRR protein that was first shown to be required for resistance 
      to Pseudomonas syringae pv. maculicola in Arabidopsis thaliana. Our previous 
      study showed that TaRPM1 gene in wheat was upregulated about six times following 
      infection by Puccinia striiformis f. sp. tritici (Pst) under high temperature, 
      compared with normal temperature. To study the function of TaRPM1 in wheat 
      high-temperature seedling-plant (HTSP) resistance to Pst, the full length of 
      TaRPM1 was cloned, with three copies each located on chromosomes 1A, 1B, and 1D. 
      Transient expression of the TaRPM1-GFP fusion protein in Nicotiana benthamiana 
      indicated that TaRPM1 localizes in the cytoplasm and nucleus. Profiling TaRPM1 
      expression indicated that TaRPM1 transcription was rapidly upregulated upon Pst 
      inoculation under high temperature. In addition, TaRPM1 was induced by exogenous 
      salicylic acid hormone application. Silencing TaRPM1 in wheat cultivar Xiaoyan 6 
      (XY 6) resulted in reduced HTSP resistance to Pst in terms of reduced number of 
      necrotic cells and increased uredinial length, whereas no obvious phenotypic 
      changes were observed in TaRPM1-silenced leaves under normal temperature. Related 
      defense genes TaPR1 and TaPR2 were downregulated in TaRPM1-silenced plants under 
      high temperature. We conclude that TaRPM1 is involved in HTSP resistance to Pst 
      in XY 6.
CI  - Copyright (c) 2020 Wang, Tian, Tao, Wang, Shang, Chen, Xu and Hu.
FAU - Wang, Jiahui
AU  - Wang J
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, China.
FAU - Tian, Wei
AU  - Tian W
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, China.
FAU - Tao, Fei
AU  - Tao F
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, China.
FAU - Wang, Jingjing
AU  - Wang J
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, China.
FAU - Shang, Hongsheng
AU  - Shang H
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, China.
FAU - Chen, Xianming
AU  - Chen X
AD  - Agricultural Research Service, United States Department of Agriculture and 
      Department of Plant Pathology, Washington State University, Pullman, WA, United 
      States.
FAU - Xu, Xiangming
AU  - Xu X
AD  - Pest & Pathogen Ecology, NIAB East Malling Research, Kent, United Kingdom.
FAU - Hu, Xiaoping
AU  - Hu X
AD  - State Key Laboratory of Crop Stress Biology for Arid Areas and College of Plant 
      Protection, Northwest A&F University, Yangling, China.
LA  - eng
PT  - Journal Article
DEP - 20200115
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC6974556
OTO - NOTNLM
OT  - NBS-LRR
OT  - high-temperature seeding plant resistance (HTSP)
OT  - non-species-specific
OT  - virus-induced gene silencing (VIGS)
OT  - wheat stripe rust
EDAT- 2020/02/06 06:00
MHDA- 2020/02/06 06:01
PMCR- 2019/01/01
CRDT- 2020/02/04 06:00
PHST- 2019/09/15 00:00 [received]
PHST- 2019/11/28 00:00 [accepted]
PHST- 2020/02/04 06:00 [entrez]
PHST- 2020/02/06 06:00 [pubmed]
PHST- 2020/02/06 06:01 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2019.01679 [doi]
PST - epublish
SO  - Front Plant Sci. 2020 Jan 15;10:1679. doi: 10.3389/fpls.2019.01679. eCollection 
      2019.