PMID- 32010569 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20220412 IS - 2218-6751 (Print) IS - 2226-4477 (Electronic) IS - 2218-6751 (Linking) VI - 8 IP - 6 DP - 2019 Dec TI - Prognostic model based on circular RNA circPDK1 for resected lung squamous cell carcinoma. PG - 907-919 LID - 10.21037/tlcr.2019.11.20 [doi] AB - BACKGROUND: Circular RNA has been revealed as a potential biomarker in multiple malignancies. However, few studies have focused on its potential to be prognostic markers in lung squamous cell carcinoma (LSCC). In this work, we aimed to build a prognostic model of resected LSCC based on circular RNA pyruvate dehydrogenase kinase 1 (circPDK1) and other clinicopathological factors. METHODS: circPDK1 was identified via next-generation sequencing. Three hundred two cases of LSCC tissue and their adjacent normal lung tissues were obtained from multiple medical centers and divided into study cohort (n=232) and validation cohort (n=70). The expression of circPDK1 was detected for analyzing its potential prognostic value for recurrence-free survival (RFS) and overall survival (OS) in LSCC. Finally, combined with circPDK1, T staging, lymph nodes (LN) metastasis status, age, and serum squamous cell Carcinoma Antigen (SCCAg), we built a prognostic model by nomograms method and confirmed it in the validation cohort. RESULTS: CircPDK1 was identified to be overexpressed (P<0.01) in LSCC. Through analysis in study cohort, circPDK1(low) patients (less than the mean expression, n=124) showed more lymph nodes metastasis (P=0.025), more vascular invasion (VI) (P=0.047), more visceral pleural invasion (VPI) (P=0.015) and poorer prognosis (P=0.003) than circPDK1(high) ones (n=108). Univariate and multivariate analysis showed that circPDK1, T staging, LN status, age, and SCCAg were signi fi cant prognostic factors for RFS and OS. The prognostic model based on these factors showed the concordance index (C-index) of 0.8214 and 0.8359 for predicting 5-year RFS and OS, respectively. Finally, the calibration curves were performed in the study cohort and a validation cohort to evaluate the model's efficiency. CONCLUSIONS: circPDK1 was identified as a potential biomarker of resected LSCC. The prognostic model including circPDK1, T staging, LN status, age, and SCCAg could effectively predict prognosis of resected LSCC. CI - 2019 Translational Lung Cancer Research. All rights reserved. FAU - Sun, Xiao AU - Sun X AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Wang, Maolong AU - Wang M AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Xu, Rongjian AU - Xu R AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Zhang, Dongyang AU - Zhang D AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Liu, Ao AU - Liu A AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Wang, Yuanyong AU - Wang Y AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Lu, Tong AU - Lu T AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Xin, Yanlu AU - Xin Y AD - Department of Endocrinology and Metabolism, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Zhao, Yandong AU - Zhao Y AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Xuan, Yunpeng AU - Xuan Y AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Qiu, Tong AU - Qiu T AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Wang, Hao AU - Wang H AD - Administrative Office, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Li, Shicheng AU - Li S AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Wo, Yang AU - Wo Y AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Liu, Dahai AU - Liu D AD - Medical Examination Center, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Zhao, Jinpeng AU - Zhao J AD - Department of Thoracic Surgery, Laiyang Central Hospital, Yantai 264000, China. FAU - Fu, Bo AU - Fu B AD - Otorhinolaryngology Head and Neck Surgery, Affiliated Qilu Hospital of Shandong University, Jinan 250000, China. FAU - Lan, Yaliang AU - Lan Y AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Han, Yudong AU - Han Y AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. FAU - Jiao, Wenjie AU - Jiao W AD - Department of Thoracic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, China. LA - eng PT - Journal Article PL - China TA - Transl Lung Cancer Res JT - Translational lung cancer research JID - 101646875 EIN - Transl Lung Cancer Res. 2020 Apr;9(2):426. PMID: 32420089 CIN - Transl Lung Cancer Res. 2020 Jun;9(3):430-431. PMID: 32676304 PMC - PMC6976365 OTO - NOTNLM OT - Circular RNA OT - lung squamous cell carcinoma (LSCC) OT - nomograms OT - prognostic model COIS- Conflicts of Interest: The authors have no conflicts of interest to declare. EDAT- 2020/02/06 06:00 MHDA- 2020/02/06 06:01 PMCR- 2019/12/01 CRDT- 2020/02/04 06:00 PHST- 2020/02/04 06:00 [entrez] PHST- 2020/02/06 06:00 [pubmed] PHST- 2020/02/06 06:01 [medline] PHST- 2019/12/01 00:00 [pmc-release] AID - tlcr-08-06-907 [pii] AID - 10.21037/tlcr.2019.11.20 [doi] PST - ppublish SO - Transl Lung Cancer Res. 2019 Dec;8(6):907-919. doi: 10.21037/tlcr.2019.11.20.