PMID- 32012270
OWN - NLM
STAT- MEDLINE
DCOM- 20210311
LR  - 20210311
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 235
IP  - 9
DP  - 2020 Sep
TI  - Effects of different fatty acids on BRL3A rat liver cell damage.
PG  - 6246-6256
LID - 10.1002/jcp.29553 [doi]
AB  - To evaluate the effects of fatty acids on endoplasmic reticulum (ER) stress, 
      oxidative stress, and lipid damage. We treated BRL3A rat liver cells with, 
      linoleic (LA), linolenic, oleic (OA), palmitic (PA), palmitoleic (POA), or 
      stearic (SA) acid for 12 hr. The characteristics of cell lipid deposition, 
      oxidative stress indexes, ER stress markers, nuclear factor kappaB p65 (NF-kappaB p65), 
      lipid synthesis and transport regulators, and cholesterol metabolism regulators 
      were analyzed. Endoplasmic chaperones like glucose-regulated protein 78, 
      CCAAT-enhancer-binding protein, NF-kappaB p65, hydrogen peroxide, and malonaldehyde 
      in PA- and SA-treated cells were significantly higher than in other treated 
      cells. Deposition of fatty acids especially LA and POA were significantly 
      increased than in other treated cells. De novo lipogenesis regulators sterol 
      regulatory element-binding protein 1c, fatty acid synthase, and acetyl-coenzyme A 
      carboxylase 1 (ACC1) expression were significantly increased in all fatty acid 
      stimulation groups, and PA- and SA-treated cells showed lower p-ACC1 expression 
      and higher scd1 expression than other fatty acid groups. Very low-density 
      lipoprotein synthesis and apolipoprotein B100 expression in free fatty acids 
      treated cells were significantly lower than control. PA, SA, OA, and POA had 
      shown significantly increased cholesterol synthesis than other treated cells. PA 
      and SA showed the lower synthesis of cytochrome P7A1 and total bile acids than 
      other fatty acids treated cells. Excess of saturated fatty acids led to severe ER 
      and oxidative stress. Excess unsaturated fatty acids led to increased lipid 
      deposition in cultured hepatocytes. A balanced fatty acid intake is needed to 
      maintain lipid homeostasis.
CI  - (c) 2020 Wiley Periodicals, Inc.
FAU - Yang, Wei
AU  - Yang W
AD  - Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, 
      Heilongjiang Bayi Agricultural University, Daqing, China.
FAU - Liu, Runqi
AU  - Liu R
AUID- ORCID: 0000-0003-0988-5274
AD  - Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, 
      Heilongjiang Bayi Agricultural University, Daqing, China.
FAU - Xia, Cheng
AU  - Xia C
AD  - Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, 
      Heilongjiang Bayi Agricultural University, Daqing, China.
FAU - Chen, Yuanyuan
AU  - Chen Y
AD  - Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, 
      Heilongjiang Bayi Agricultural University, Daqing, China.
FAU - Dong, Zhihao
AU  - Dong Z
AD  - Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, 
      Heilongjiang Bayi Agricultural University, Daqing, China.
FAU - Huang, Baoyin
AU  - Huang B
AD  - Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, 
      Heilongjiang Bayi Agricultural University, Daqing, China.
FAU - Li, Ruirui
AU  - Li R
AD  - Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, 
      Heilongjiang Bayi Agricultural University, Daqing, China.
FAU - Li, Ming
AU  - Li M
AD  - Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, 
      Heilongjiang Bayi Agricultural University, Daqing, China.
FAU - Xu, Chuang
AU  - Xu C
AD  - Clinical Veterinary Medicine, College of Animal Science and Veterinary Medicine, 
      Heilongjiang Bayi Agricultural University, Daqing, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200203
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Fatty Acids)
RN  - 0 (Lipids)
RN  - 0 (Lipoproteins, VLDL)
RN  - 0 (Stearic Acids)
RN  - 0RBV727H71 (alpha-Linolenic Acid)
RN  - 2UMI9U37CP (Oleic Acid)
RN  - 2V16EO95H1 (Palmitic Acid)
RN  - 4ELV7Z65AP (stearic acid)
RN  - 9KJL21T0QJ (Linoleic Acid)
RN  - EC 2.3.1.85 (Fatty Acid Synthases)
RN  - EC 6.4.1.2 (Acetyl-CoA Carboxylase)
SB  - IM
MH  - Acetyl-CoA Carboxylase/genetics
MH  - Animals
MH  - Cells, Cultured
MH  - Endoplasmic Reticulum Stress/*drug effects
MH  - Fatty Acid Synthases/genetics
MH  - Fatty Acids/metabolism/*pharmacology
MH  - Fatty Liver/*drug therapy/metabolism/pathology
MH  - Hepatocytes/drug effects/metabolism
MH  - Humans
MH  - Linoleic Acid/pharmacology
MH  - Lipid Metabolism/drug effects
MH  - Lipids/biosynthesis/genetics
MH  - Lipogenesis/drug effects/*genetics
MH  - Lipoproteins, VLDL/genetics
MH  - Liver/drug effects/metabolism
MH  - Oleic Acid/pharmacology
MH  - Oxidative Stress/drug effects
MH  - Palmitic Acid/pharmacology
MH  - Rats
MH  - Stearic Acids/pharmacology
MH  - alpha-Linolenic Acid/pharmacology
OTO - NOTNLM
OT  - fatty acids
OT  - lipid deposition
OT  - nonalcoholic fatty liver disease
OT  - oxidative stress
OT  - reticulum stress
EDAT- 2020/02/06 06:00
MHDA- 2021/03/12 06:00
CRDT- 2020/02/04 06:00
PHST- 2018/12/08 00:00 [received]
PHST- 2020/01/10 00:00 [accepted]
PHST- 2020/02/06 06:00 [pubmed]
PHST- 2021/03/12 06:00 [medline]
PHST- 2020/02/04 06:00 [entrez]
AID - 10.1002/jcp.29553 [doi]
PST - ppublish
SO  - J Cell Physiol. 2020 Sep;235(9):6246-6256. doi: 10.1002/jcp.29553. Epub 2020 Feb 
      3.