PMID- 32012427
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1613-4133 (Electronic)
IS  - 1613-4125 (Linking)
VI  - 64
IP  - 8
DP  - 2020 Apr
TI  - Aspalathin Protects Insulin-Producing beta Cells against Glucotoxicity and Oxidative 
      Stress-Induced Cell Death.
PG  - e1901009
LID - 10.1002/mnfr.201901009 [doi]
AB  - SCOPE: Aspalathin, the main polyphenolic phytochemical of rooibos (Aspalathus 
      linearis), has been attributed with health promoting properties, including a 
      glucose lowering effect that can prove interesting for application as 
      nutraceutical or therapeutic in (pre-)diabetics. Preservation of beta cell mass in 
      the pancreas is considered a key issue for diabetes prevention or treatment, 
      therefore the aim is to investigate whether aspalathin also has beta cell 
      cytoprotective potential. METHODS AND RESULTS: Rat pancreatic islets and the beta 
      cell line Insulinoma 1E (INS1E) are studied in vitro after exposure to various 
      cytotoxic agents, namely streptozotocin (STZ), hydrogen peroxide, or chronic high 
      glucose. The effect of aspalathin on cell survival and apoptosis is studied. 
      Expression of relevant cytoprotective genes is analyzed by qRT-PCR and proteins 
      by Western blot. Aspalathin is found to protect beta cells against cytotoxicity and 
      apoptosis. This is associated with increased translocation of nuclear factor 
      erythroid 2-related factor 2 (NRF2) and expression of its antioxidant target 
      genes heme oxygenase 1 (Hmox1), NAD(P)H quinone dehydrogenase 1 (Nqo-1), and 
      superoxide dismutase 1 (Sod1). CONCLUSION: It is proposed that aspalathin 
      protects beta cells against glucotoxicity and oxidative stress by increasing the 
      expression of NRF2-regulated antioxidant enzymes. This indicates that aspalathin 
      is an interesting beta cell cytoprotectant.
CI  - (c) 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Moens, Celine
AU  - Moens C
AUID- ORCID: 0000-0002-4125-4950
AD  - Cell Differentiation Lab, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 
      Jette, Brussels, Belgium.
FAU - Bensellam, Mohammed
AU  - Bensellam M
AD  - Universite Catholique de Louvain, Institut de Recherche Experimentale et 
      Clinique, Pole d'Endocrinologie, Diabete et Nutrition, Avenue Hippocrate 55, 1200 
      Brussels, Brussels, Belgium.
FAU - Himpe, Eddy
AU  - Himpe E
AD  - Cell Differentiation Lab, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 
      Jette, Brussels, Belgium.
FAU - Muller, Christo J F
AU  - Muller CJF
AD  - Biomedical Research and Innovation Platform, South African Medical Research 
      Council, Francie van Zijl Drive, 7505, Western Cape, Tygerberg, South Africa.
FAU - Jonas, Jean-Christophe
AU  - Jonas JC
AD  - Universite Catholique de Louvain, Institut de Recherche Experimentale et 
      Clinique, Pole d'Endocrinologie, Diabete et Nutrition, Avenue Hippocrate 55, 1200 
      Brussels, Brussels, Belgium.
FAU - Bouwens, Luc
AU  - Bouwens L
AD  - Cell Differentiation Lab, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 
      Jette, Brussels, Belgium.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200212
PL  - Germany
TA  - Mol Nutr Food Res
JT  - Molecular nutrition & food research
JID - 101231818
RN  - 0 (Chalcones)
RN  - 0 (Protective Agents)
RN  - 0 (aspalathin)
RN  - 5W494URQ81 (Streptozocin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
RN  - EC 1.14.14.18 (Hmox1 protein, rat)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Cell Death/drug effects
MH  - Cells, Cultured
MH  - Chalcones/administration & dosage/*pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression Regulation/drug effects
MH  - Glucose/toxicity
MH  - Heme Oxygenase (Decyclizing)/genetics
MH  - Hydrogen Peroxide/toxicity
MH  - Insulin-Secreting Cells/*drug effects
MH  - Male
MH  - Oxidative Stress/*drug effects/genetics
MH  - Protective Agents/*pharmacology
MH  - Rats, Wistar
MH  - Streptozocin/toxicity
OTO - NOTNLM
OT  - apoptosis
OT  - aspalathin
OT  - diabetes
OT  - glucotoxicity
OT  - oxidative stress
EDAT- 2020/02/06 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/02/04 06:00
PHST- 2019/09/23 00:00 [received]
PHST- 2019/12/23 00:00 [revised]
PHST- 2020/02/06 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/02/04 06:00 [entrez]
AID - 10.1002/mnfr.201901009 [doi]
PST - ppublish
SO  - Mol Nutr Food Res. 2020 Apr;64(8):e1901009. doi: 10.1002/mnfr.201901009. Epub 
      2020 Feb 12.