PMID- 32012657
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2223-7747 (Print)
IS  - 2223-7747 (Electronic)
IS  - 2223-7747 (Linking)
VI  - 9
IP  - 2
DP  - 2020 Jan 27
TI  - Community Structure, Diversity and Potential of Endophytic Bacteria in the 
      Primitive New Zealand Medicinal Plant Pseudowintera colorata.
LID - 10.3390/plants9020156 [doi]
LID - 156
AB  - Although the importance of the plant microbiome in commercial plant health has 
      been well established, there are limited studies in native medicinal plants. 
      Pseudowintera colorata (horopito) is a native New Zealand medicinal plant 
      recognized for its antimicrobial properties. Denaturing gradient gel 
      electrophoresis (DGGE) and Illumina MiSeq analysis of P. colorata plants from ten 
      sites across New Zealand showed that tissue type strongly influenced the 
      diversity and richness of endophytic bacteria (PERMANOVA, P < 0.05). In addition, 
      two OTUs belonging to the genus Pseudomonas (Greengenes ID: 646549 and 138914) 
      were found to be present in >75% of all P. colorata leaf, stem and root samples 
      and were identified as the members of the P. colorata "core endomicrobiome". 
      Culture-independent analysis was complemented by the recovery of 405 endophytic 
      bacteria from the tissues of P. colorata. Some of these cultured endophytic 
      bacteria (n = 10) showed high antagonism against four different phytopathogenic 
      fungi tested. The influence of endophytic bacteria on plant growth was assessed 
      by inoculating P. colorata seedlings. The mean shoot height of seedlings treated 
      with Bacillus sp. TP1LA1B were longer (1.83x), had higher shoot dry weight (1.8x) 
      and produced more internodes (1.8x) compared to the control.
FAU - Purushotham, Neeraj
AU  - Purushotham N
AD  - Department of Pest-Management and Conservation, Lincoln University, Lincoln 7647, 
      New Zealand.
FAU - Jones, Eirian
AU  - Jones E
AD  - Department of Pest-Management and Conservation, Lincoln University, Lincoln 7647, 
      New Zealand.
FAU - Monk, Jana
AU  - Monk J
AD  - AsureQuality, Lincoln 7647, New Zealand.
FAU - Ridgway, Hayley
AU  - Ridgway H
AD  - Department of Pest-Management and Conservation, Lincoln University, Lincoln 7647, 
      New Zealand.
AD  - The New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, New 
      Zealand.
LA  - eng
PT  - Journal Article
DEP - 20200127
PL  - Switzerland
TA  - Plants (Basel)
JT  - Plants (Basel, Switzerland)
JID - 101596181
PMC - PMC7076676
OTO - NOTNLM
OT  - Illumina MiSeq
OT  - endophytes
OT  - endophytic bacteria
OT  - medicinal plant
OT  - microbial ecology
OT  - plant growth promotion
OT  - plant-microbe interactions
COIS- The authors declare no conflict of interest.
EDAT- 2020/02/06 06:00
MHDA- 2020/02/06 06:01
PMCR- 2020/01/27
CRDT- 2020/02/05 06:00
PHST- 2019/12/27 00:00 [received]
PHST- 2020/01/15 00:00 [revised]
PHST- 2020/01/23 00:00 [accepted]
PHST- 2020/02/05 06:00 [entrez]
PHST- 2020/02/06 06:00 [pubmed]
PHST- 2020/02/06 06:01 [medline]
PHST- 2020/01/27 00:00 [pmc-release]
AID - plants9020156 [pii]
AID - plants-09-00156 [pii]
AID - 10.3390/plants9020156 [doi]
PST - epublish
SO  - Plants (Basel). 2020 Jan 27;9(2):156. doi: 10.3390/plants9020156.