PMID- 32013038
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240328
IS  - 2079-4991 (Print)
IS  - 2079-4991 (Electronic)
IS  - 2079-4991 (Linking)
VI  - 10
IP  - 2
DP  - 2020 Jan 28
TI  - Few Layer Graphene Does Not Affect Cellular Homeostasis of Mouse Macrophages.
LID - 10.3390/nano10020228 [doi]
LID - 228
AB  - : Graphene-related materials (GRMs) are widely used in various applications due 
      to their unique properties. A growing number of reports describe the impact of 
      different carbon nanomaterials, including graphene oxide (GO), reduced GO (rGO), 
      and carbon nanotubes (CNT), on immune cells, but there is still a very limited 
      number of studies on graphene. In this work, we investigated the biological 
      responses of few layer graphene (FLG) on mouse macrophages (bone marrow derived 
      macrophages, BMDMs), which are part of the first line of defense in innate 
      immunity. In particular, our paper describes our findings of short-term FLG 
      treatment in BMDMs with a focus on observing material internalization and changes 
      in general cell morphology. Subsequent investigation of cytotoxicity parameters 
      showed that increasing doses of FLG did not hamper the viability of cells and did 
      not trigger inflammatory responses. Basal level induced autophagic activity 
      sufficed to maintain the cellular homeostasis of FLG treated cells. Our results 
      shed light on the impact of FLG on primary macrophages and show that FLG does not 
      elicit immunological responses leading to cell death.
FAU - Malanagahalli, Sowmya
AU  - Malanagahalli S
AD  - CNRS, UPR3572, Immunology, Immunopathology and Therapeutic Chemistry, University 
      of Strasbourg, ISIS, 67000 Strasbourg, France.
FAU - Murera, Diane
AU  - Murera D
AD  - CNRS, UPR3572, Immunology, Immunopathology and Therapeutic Chemistry, University 
      of Strasbourg, ISIS, 67000 Strasbourg, France.
FAU - Martin, Cristina
AU  - Martin C
AD  - CNRS, UPR3572, Immunology, Immunopathology and Therapeutic Chemistry, University 
      of Strasbourg, ISIS, 67000 Strasbourg, France.
AD  - Instituto Regional de Investigacion Cientifica Aplicada (IRICA), Universidad de 
      Castilla-La Mancha, Avda Camilo Jose Cela, 13071 Ciudad Real, Spain.
FAU - Lin, Hazel
AU  - Lin H
AD  - CNRS, UPR3572, Immunology, Immunopathology and Therapeutic Chemistry, University 
      of Strasbourg, ISIS, 67000 Strasbourg, France.
FAU - Wadier, Nadege
AU  - Wadier N
AD  - CNRS, UPR3572, Immunology, Immunopathology and Therapeutic Chemistry, University 
      of Strasbourg, ISIS, 67000 Strasbourg, France.
FAU - Dumortier, Helene
AU  - Dumortier H
AD  - CNRS, UPR3572, Immunology, Immunopathology and Therapeutic Chemistry, University 
      of Strasbourg, ISIS, 67000 Strasbourg, France.
FAU - Vazquez, Ester
AU  - Vazquez E
AD  - Instituto Regional de Investigacion Cientifica Aplicada (IRICA), Universidad de 
      Castilla-La Mancha, Avda Camilo Jose Cela, 13071 Ciudad Real, Spain.
AD  - Departamento de Quimica Organica, Facultad de Ciencias y Tecnologias Quimicas, 
      Universidad de Castilla-La Mancha, 13071 Ciudad Real, Spain.
FAU - Bianco, Alberto
AU  - Bianco A
AD  - CNRS, UPR3572, Immunology, Immunopathology and Therapeutic Chemistry, University 
      of Strasbourg, ISIS, 67000 Strasbourg, France.
LA  - eng
GR  - 696656 and 785219/EU Graphene Flagship/
GR  - ANR-10-LABX-0026_CSC/Agence Nationale de la Recherche/
GR  - ANR-11-EQPX-022/Agence Nationale de la Recherche/
GR  - CTQ2017-88158-R/Spanish government/
PT  - Journal Article
DEP - 20200128
PL  - Switzerland
TA  - Nanomaterials (Basel)
JT  - Nanomaterials (Basel, Switzerland)
JID - 101610216
PMC - PMC7074970
OTO - NOTNLM
OT  - autophagy
OT  - bone marrow derived macrophages
OT  - carbon nanomaterials
OT  - graphene
OT  - primary immune cells
COIS- The authors declare no conflicts of interest.
EDAT- 2020/02/06 06:00
MHDA- 2020/02/06 06:01
PMCR- 2020/01/28
CRDT- 2020/02/05 06:00
PHST- 2019/12/18 00:00 [received]
PHST- 2020/01/16 00:00 [revised]
PHST- 2020/01/17 00:00 [accepted]
PHST- 2020/02/05 06:00 [entrez]
PHST- 2020/02/06 06:00 [pubmed]
PHST- 2020/02/06 06:01 [medline]
PHST- 2020/01/28 00:00 [pmc-release]
AID - nano10020228 [pii]
AID - nanomaterials-10-00228 [pii]
AID - 10.3390/nano10020228 [doi]
PST - epublish
SO  - Nanomaterials (Basel). 2020 Jan 28;10(2):228. doi: 10.3390/nano10020228.