PMID- 32013257
OWN - NLM
STAT- MEDLINE
DCOM- 20201030
LR  - 20211204
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 3
DP  - 2020 Jan 29
TI  - Cationic Polymer Nanoparticles-Mediated Delivery of miR-124 Impairs 
      Tumorigenicity of Prostate Cancer Cells.
LID - 10.3390/ijms21030869 [doi]
LID - 869
AB  - MicroRNAs (miRNAs) play a pivotal role in regulating the expression of genes 
      involved in tumor development, invasion, and metastasis. In particular, 
      microRNA-124 (miR-124) modulates the expression of carnitine palmitoyltransferase 
      1A (CPT1A) at the post-transcriptional level, impairing the ability of 
      androgen-independent prostate cancer (PC3) cells to completely metabolize lipid 
      substrates. However, the clinical translation of miRNAs requires the development 
      of effective and safe delivery systems able to protect nucleic acids from 
      degradation. Herein, biodegradable polyethyleneimine-functionalized 
      polyhydroxybutyrate nanoparticles (PHB-PEI NPs) were prepared by aminolysis and 
      used as cationic non-viral vectors to complex and deliver miR-124 in PC3 cells. 
      Notably, the PHB-PEI NPs/miRNA complex effectively protected miR-124 from RNAse 
      degradation, resulting in a 30% increase in delivery efficiency in PC3 cells 
      compared to a commercial transfection agent (Lipofectamine RNAiMAX). Furthermore, 
      the NPs-delivered miR-124 successfully impaired hallmarks of tumorigenicity, such 
      as cell proliferation, motility, and colony formation, through CPT1A modulation. 
      These results demonstrate that the use of PHB-PEI NPs represents a suitable and 
      convenient strategy to develop novel nanomaterials with excellent 
      biocompatibility and high transfection efficiency for cancer therapy.
FAU - Conte, Raffaele
AU  - Conte R
AD  - Research Institute on Terrestrial Ecosystems (IRET)-CNR, Via Pietro Castellino 
      111, 80131 Naples, Italy.
FAU - Valentino, Anna
AU  - Valentino A
AD  - Elleva Pharma s.r.l. via P. Castellino, 111 - 80131 Naples, Italy.
FAU - Di Cristo, Francesca
AU  - Di Cristo F
AD  - Elleva Pharma s.r.l. via P. Castellino, 111 - 80131 Naples, Italy.
FAU - Peluso, Gianfranco
AU  - Peluso G
AD  - Research Institute on Terrestrial Ecosystems (IRET)-CNR, Via Pietro Castellino 
      111, 80131 Naples, Italy.
FAU - Cerruti, Pierfrancesco
AU  - Cerruti P
AD  - Institute for Polymers, Composites and Biomaterials (IPCB-CNR) Via Campi Flegrei, 
      34, 80078 Pozzuoli (NA), Italy.
FAU - Di Salle, Anna
AU  - Di Salle A
AUID- ORCID: 0000-0001-6763-3636
AD  - Research Institute on Terrestrial Ecosystems (IRET)-CNR, Via Pietro Castellino 
      111, 80131 Naples, Italy.
FAU - Calarco, Anna
AU  - Calarco A
AUID- ORCID: 0000-0002-1441-7662
AD  - Research Institute on Terrestrial Ecosystems (IRET)-CNR, Via Pietro Castellino 
      111, 80131 Naples, Italy.
LA  - eng
GR  - 201288JKYY_003/Italian Ministry of University and Research/
GR  - PON 03 PE_00110_1/ptd1_000410/Regione Campania/
GR  - B93D18000350007/POR Campania FESR 2014_2020/
PT  - Journal Article
DEP - 20200129
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Drug Carriers)
RN  - 0 (MIRN124 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (PHB protein, human)
RN  - 0 (Prohibitins)
SB  - IM
MH  - Caco-2 Cells
MH  - Carcinogenesis/*metabolism/pathology
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - *Drug Carriers/chemistry/pharmacology
MH  - Humans
MH  - MCF-7 Cells
MH  - Male
MH  - *MicroRNAs/chemistry/pharmacology
MH  - Nanoparticles/*chemistry
MH  - PC-3 Cells
MH  - Prohibitins
MH  - Prostatic Neoplasms/*metabolism/pathology
PMC - PMC7038067
OTO - NOTNLM
OT  - aminolysis
OT  - gene delivery
OT  - miR-124
OT  - polyethyleneimine (PEI)
OT  - polyhydroxybutyrate (PHB)
OT  - polymer nanoparticles
OT  - prostate cancer
COIS- The authors declare no conflict of interest.
EDAT- 2020/02/06 06:00
MHDA- 2020/10/31 06:00
PMCR- 2020/02/01
CRDT- 2020/02/05 06:00
PHST- 2020/01/16 00:00 [received]
PHST- 2020/01/27 00:00 [revised]
PHST- 2020/01/28 00:00 [accepted]
PHST- 2020/02/05 06:00 [entrez]
PHST- 2020/02/06 06:00 [pubmed]
PHST- 2020/10/31 06:00 [medline]
PHST- 2020/02/01 00:00 [pmc-release]
AID - ijms21030869 [pii]
AID - ijms-21-00869 [pii]
AID - 10.3390/ijms21030869 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Jan 29;21(3):869. doi: 10.3390/ijms21030869.