PMID- 32014588
OWN - NLM
STAT- MEDLINE
DCOM- 20201130
LR  - 20201130
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 99
DP  - 2020 Apr
TI  - Characterization and bioactivity of grass carp (Ctenopharyngodon idella) 
      interleukin-21: Inducible production and involvement in inflammatory regulation.
PG  - 19-26
LID - S1050-4648(20)30067-X [pii]
LID - 10.1016/j.fsi.2020.01.059 [doi]
AB  - In mammals, interleukin 21 (IL-21) is a broad pleiotropic cytokine that plays 
      critical roles in the development of several inflammatory and autoimmune 
      diseases. In fish, functional information of Il-21 is limited, and its role in 
      immune response is largely unknown. In the present study, we cloned a coding 
      sequence of grass carp (Ctenopharyngodon idella) il21 gene (gcil21). To 
      characterize the release patterns and biological activity of gcIl-21, we prepared 
      recombinant gcIl-21 (rgcIl-21) and obtained the polyclonal antibody with gcIl-21 
      specificity. Western blotting analysis showed that in grass carp head kidney 
      leukocytes (HKLs), gcIl-21 was undetected in culture supernatant of untreated 
      cells but drastically induced by heat-killed Aeromonas hydrophila (A. 
      hydrophila), uncovering the release features of gcIl-21 and its possible 
      involvement in immune response. Subsequent functional experiments revealed that 
      rgcIl-21 did not affect the mRNA expression of grass carp il1b and tgfb, but 
      induced a strong expression of grass carp il10, and to a lesser extent of grass 
      carp tnfa in HKLs, suggesting a dominant effect of gcIl-21 in modulating Il-10 
      signaling as seen in rainbow trout and mammals. Furthermore, in vivo studies 
      showed that intraperitoneal injection of rgcIl-21 was able to increase the 
      survival rate of grass carp infected with live A. hydrophila, and reduce the 
      pathological responses caused by the same pathogenic bacteria in head kidney and 
      intestine. Taken together, these results for the first time revealed the close 
      relationship of fish Il-21 production and function with inflammatory responses, 
      and highlighted its anti-bacterial and anti-inflammatory ability, thereby 
      providing a new insight into host defense mechanisms in fish.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Zhang, Anying
AU  - Zhang A
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Jian, Xiaoyu
AU  - Jian X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Wang, Dan
AU  - Wang D
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Ren, Jingqi
AU  - Ren J
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Wang, Xinyan
AU  - Wang X
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China.
FAU - Zhou, Hong
AU  - Zhou H
AD  - School of Life Science and Technology, University of Electronic Science and 
      Technology of China, Chengdu, People's Republic of China. Electronic address: 
      zhouhongzh@uestc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200131
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Fish Proteins)
RN  - 0 (Interleukins)
RN  - 0 (Recombinant Proteins)
RN  - 130068-27-8 (Interleukin-10)
RN  - MKM3CA6LT1 (interleukin-21)
SB  - IM
MH  - Aeromonas hydrophila/immunology
MH  - Animals
MH  - Bacterial Infections/immunology/*veterinary
MH  - Carps/*immunology/microbiology
MH  - Cells, Cultured
MH  - Cloning, Molecular
MH  - Fish Diseases/immunology/microbiology
MH  - Fish Proteins/genetics/*immunology
MH  - Gene Expression Regulation
MH  - Head Kidney/cytology/immunology
MH  - Inflammation/*genetics
MH  - Interleukin-10/genetics/immunology
MH  - Interleukins/genetics/*immunology
MH  - Leukocytes/immunology/microbiology
MH  - Recombinant Proteins/genetics/immunology
OTO - NOTNLM
OT  - Anti-bacterial activity
OT  - Anti-inflammatory property
OT  - Grass carp
OT  - Il-21
OT  - Protein release
EDAT- 2020/02/06 06:00
MHDA- 2020/12/01 06:00
CRDT- 2020/02/05 06:00
PHST- 2019/09/17 00:00 [received]
PHST- 2020/01/27 00:00 [revised]
PHST- 2020/01/28 00:00 [accepted]
PHST- 2020/02/06 06:00 [pubmed]
PHST- 2020/12/01 06:00 [medline]
PHST- 2020/02/05 06:00 [entrez]
AID - S1050-4648(20)30067-X [pii]
AID - 10.1016/j.fsi.2020.01.059 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2020 Apr;99:19-26. doi: 10.1016/j.fsi.2020.01.059. Epub 
      2020 Jan 31.