PMID- 32014594 OWN - NLM STAT- MEDLINE DCOM- 20200819 LR - 20200819 IS - 1743-9159 (Electronic) IS - 1743-9159 (Linking) VI - 75 DP - 2020 Mar TI - Impact of sleeve gastrectomy and dietary change on metabolic and hepatic function in an obesity rat model - Experimental research. PG - 139-147 LID - S1743-9191(20)30150-3 [pii] LID - 10.1016/j.ijsu.2020.01.139 [doi] AB - PURPOSE: Sleeve gastrectomy (SG) is an effective procedure to treat morbid obesity. SG induces remission of type 2 diabetes and metabolic syndrome and improves non-alcoholic fatty liver disease (NAFLD). However, it is imperative to clarify the extent to which these beneficial effects may be attributed to SG among other concomitant changes including postoperative diet. The current study addresses this question in a rodent model of obesity by subjecting it to SG, normal diet, or a combination of the two. METHODS: Male Wistar-rats were fed with either high fat diet (HF, n = 32) or received chow diet (CD, n = 8). After 15 weeks, the HF-fed rats underwent either SG or sham operation, following which they were randomised to either continue HF or switched to CD for another 6 weeks. Body weight, fasting blood glucose level, blood pressure, and adipokine expression (leptin, adiponectin, MCP-1) in the adipose tissue along with triglycerides level in the blood serum were assessed to evaluate metabolic function. Hepatic function was assessed by histological evaluation of liver fibrosis (Hydroxyproline, Sirius Red) and reverse transcription polymerase chain reaction (RT-PCR) of the inflammation marker monocyte chemoattractant protein-1 (MCP-1). RESULTS: Postoperative dietary change improved adipose tissue inflammation and arterial blood pressure regardless of the surgical intervention, while SG improved hyperglycaemia, blood triglyceride levels and, regardless of the postoperative diet, hepatic inflammation and fibrosis. However, combined administration of SG with post-operative normal diet was the most effective with regard to reducing the body weight. CONCLUSION: HF for 15 weeks induced obesity with metabolic syndrome and NAFLD in rats. SG and dietary intervention improved metabolic state and NAFLD; however, their combination was significantly more effective. CI - Copyright (c) 2020 IJS Publishing Group Ltd. Published by Elsevier Ltd. All rights reserved. FAU - Dohmen, Jonas AU - Dohmen J AD - Department of Surgery, University of Bonn, Venusberg-Campus 1, 53127, Bonn, Germany. Electronic address: Jonas.Dohmen@ukbonn.de. FAU - Praktiknjo, Michael AU - Praktiknjo M AD - Department of Internal Medicine I, University of Bonn, Venusberg-Campus 1, 53127, Bonn, Germany. Electronic address: Michael.Praktiknjo@ukbonn.de. FAU - Rudeloff, Anna AU - Rudeloff A AD - Department of Surgery, University of Bonn, Venusberg-Campus 1, 53127, Bonn, Germany. Electronic address: annarudeloff@googlemail.com. FAU - Uschner, Frank Erhard AU - Uschner FE AD - Department of Internal Medicine I, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590, Frankfurt Am Main, Germany. Electronic address: Frank.Uschner@kgu.de. FAU - Klein, Sabine AU - Klein S AD - Department of Internal Medicine I, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590, Frankfurt Am Main, Germany. Electronic address: Sabine.Klein@kgu.de. FAU - Plamper, Andreas AU - Plamper A AD - Department of Bariatric, Metabolic and Plastic Surgery, St. Franziskus-Hospital, Schonsteinstr. 63, 50825, Cologne, Germany. Electronic address: Andreas.Plamper@cellitinnen.de. FAU - Matthaei, Hanno AU - Matthaei H AD - Department of Surgery, University of Bonn, Venusberg-Campus 1, 53127, Bonn, Germany. Electronic address: Hanno.Matthaei@ukbonn.de. FAU - Rheinwalt, Karl-Peter AU - Rheinwalt KP AD - Department of Bariatric, Metabolic and Plastic Surgery, St. Franziskus-Hospital, Schonsteinstr. 63, 50825, Cologne, Germany. Electronic address: KarlPeter.Rheinwalt@cellitinnen.de. FAU - Wehner, Sven AU - Wehner S AD - Department of Surgery, University of Bonn, Venusberg-Campus 1, 53127, Bonn, Germany. Electronic address: Sven.Wehner@ukbonn.de. FAU - Kalff, Jorg C AU - Kalff JC AD - Department of Surgery, University of Bonn, Venusberg-Campus 1, 53127, Bonn, Germany. Electronic address: kalff@uni-bonn.de. FAU - Trebicka, Jonel AU - Trebicka J AD - Department of Internal Medicine I, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590, Frankfurt Am Main, Germany; European Foundation for the Study of Chronic Liver Failure - EF Clif, Travessera de Gracia, 11, 08021, Barcelona, Spain. Electronic address: Jonel.Trebicka@kgu.de. FAU - Lingohr, Philipp AU - Lingohr P AD - Department of Surgery, University of Bonn, Venusberg-Campus 1, 53127, Bonn, Germany. Electronic address: Philipp.Lingohr@ukbonn.de. LA - eng PT - Journal Article DEP - 20200201 PL - United States TA - Int J Surg JT - International journal of surgery (London, England) JID - 101228232 SB - IM CIN - Int J Surg. 2020 Apr;76:77-78. PMID: 32109652 CIN - Int J Surg. 2020 Apr;76:69. PMID: 32112976 MH - Adipose Tissue/metabolism/pathology MH - Animals MH - Diet, High-Fat MH - Disease Models, Animal MH - Gastrectomy/*methods MH - Liver/*physiopathology MH - Male MH - Metabolic Syndrome/therapy MH - Non-alcoholic Fatty Liver Disease/therapy MH - Obesity, Morbid/physiopathology/*surgery MH - Rats MH - Rats, Wistar OTO - NOTNLM OT - Adipokines OT - Bariatric surgery OT - NAFLD OT - NASH OT - T2DM COIS- Declaration of competing interest All authors declare, that they do not have conflicts of interests. EDAT- 2020/02/06 06:00 MHDA- 2020/08/20 06:00 CRDT- 2020/02/05 06:00 PHST- 2019/11/30 00:00 [received] PHST- 2020/01/15 00:00 [revised] PHST- 2020/01/26 00:00 [accepted] PHST- 2020/02/06 06:00 [pubmed] PHST- 2020/08/20 06:00 [medline] PHST- 2020/02/05 06:00 [entrez] AID - S1743-9191(20)30150-3 [pii] AID - 10.1016/j.ijsu.2020.01.139 [doi] PST - ppublish SO - Int J Surg. 2020 Mar;75:139-147. doi: 10.1016/j.ijsu.2020.01.139. Epub 2020 Feb 1.