PMID- 32015496
OWN - NLM
STAT- MEDLINE
DCOM- 20200720
LR  - 20230906
IS  - 2058-5276 (Electronic)
IS  - 2058-5276 (Linking)
VI  - 5
IP  - 3
DP  - 2020 Mar
TI  - Spatial metabolomics of in situ host-microbe interactions at the micrometre 
      scale.
PG  - 498-510
LID - 10.1038/s41564-019-0664-6 [doi]
AB  - Spatial metabolomics describes the location and chemistry of small molecules 
      involved in metabolic phenotypes, defence molecules and chemical interactions in 
      natural communities. Most current techniques are unable to spatially link the 
      genotype and metabolic phenotype of microorganisms in situ at a scale relevant to 
      microbial interactions. Here, we present a spatial metabolomics pipeline 
      (metaFISH) that combines fluorescence in situ hybridization (FISH) microscopy and 
      high-resolution atmospheric-pressure matrix-assisted laser desorption/ionization 
      mass spectrometry to image host-microbe symbioses and their metabolic 
      interactions. The metaFISH pipeline aligns and integrates metabolite and 
      fluorescent images at the micrometre scale to provide a spatial assignment of 
      host and symbiont metabolites on the same tissue section. To illustrate the 
      advantages of metaFISH, we mapped the spatial metabolome of a deep-sea mussel and 
      its intracellular symbiotic bacteria at the scale of individual epithelial host 
      cells. Our analytical pipeline revealed metabolic adaptations of the epithelial 
      cells to the intracellular symbionts and variation in metabolic phenotypes within 
      a single symbiont 16S rRNA phylotype, and enabled the discovery of specialized 
      metabolites from the host-microbe interface. metaFISH provides a 
      culture-independent approach to link metabolic phenotypes to community members in 
      situ and is a powerful tool for microbiologists across fields.
FAU - Geier, Benedikt
AU  - Geier B
AUID- ORCID: 0000-0002-2942-2624
AD  - Max Planck Institute for Marine Microbiology, Bremen, Germany. 
      bgeier@mpi-bremen.de.
FAU - Sogin, Emilia M
AU  - Sogin EM
AD  - Max Planck Institute for Marine Microbiology, Bremen, Germany.
FAU - Michellod, Dolma
AU  - Michellod D
AD  - Max Planck Institute for Marine Microbiology, Bremen, Germany.
FAU - Janda, Moritz
AU  - Janda M
AD  - Max Planck Institute for Marine Microbiology, Bremen, Germany.
FAU - Kompauer, Mario
AU  - Kompauer M
AD  - Institute of Inorganic and Analytical Chemistry, Justus Liebig University 
      Giessen, Giessen, Germany.
FAU - Spengler, Bernhard
AU  - Spengler B
AD  - Institute of Inorganic and Analytical Chemistry, Justus Liebig University 
      Giessen, Giessen, Germany.
FAU - Dubilier, Nicole
AU  - Dubilier N
AUID- ORCID: 0000-0002-9394-825X
AD  - Max Planck Institute for Marine Microbiology, Bremen, Germany.
AD  - MARUM, University of Bremen, Bremen, Germany.
FAU - Liebeke, Manuel
AU  - Liebeke M
AUID- ORCID: 0000-0002-2339-1409
AD  - Max Planck Institute for Marine Microbiology, Bremen, Germany. 
      mliebeke@mpi-bremen.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200203
PL  - England
TA  - Nat Microbiol
JT  - Nature microbiology
JID - 101674869
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/genetics/metabolism
MH  - Bivalvia/*microbiology
MH  - Host Microbial Interactions/*physiology
MH  - In Situ Hybridization, Fluorescence
MH  - Metabolome
MH  - Metabolomics/*methods
MH  - Microbiota/genetics/*physiology
MH  - RNA, Ribosomal, 16S/genetics
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
MH  - Symbiosis
MH  - Tandem Mass Spectrometry
EDAT- 2020/02/06 06:00
MHDA- 2020/07/21 06:00
CRDT- 2020/02/05 06:00
PHST- 2019/03/04 00:00 [received]
PHST- 2019/12/16 00:00 [accepted]
PHST- 2020/02/06 06:00 [pubmed]
PHST- 2020/07/21 06:00 [medline]
PHST- 2020/02/05 06:00 [entrez]
AID - 10.1038/s41564-019-0664-6 [pii]
AID - 10.1038/s41564-019-0664-6 [doi]
PST - ppublish
SO  - Nat Microbiol. 2020 Mar;5(3):498-510. doi: 10.1038/s41564-019-0664-6. Epub 2020 
      Feb 3.