PMID- 32022063
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1473-0189 (Electronic)
IS  - 1473-0189 (Linking)
VI  - 20
IP  - 5
DP  - 2020 Mar 3
TI  - Mass production of thin-walled hollow optical fibers enables disposable 
      optofluidic laser immunosensors.
PG  - 923-930
LID - 10.1039/c9lc01216h [doi]
AB  - Disposable biosensors are of great importance in disease diagnosis due to their 
      inherent merits of no cross-contamination and ease of use. Optofluidic laser 
      (OFL) sensors are a new category of sensitive biosensors; however, it is 
      challenging to cost-effectively mass-produce them to achieve disposability. Here, 
      we report a disposable optofluidic laser immunosensor based on thin-walled hollow 
      optical fibers (HOFs). Using a fiber draw tower, the fabrication parameters, 
      including drawing speed and gas flow rate, are explored, and the HOF geometry is 
      precisely controlled, which allows identical laser microring resonators to be 
      distributed along the fibers. The disposable OFL immunosensor detects the protein 
      concentration in the HOF through a wash-free immunoassay. Enabled by the 
      disposable sensors, the statistical characteristics of 80 tests for each 
      concentration greatly reduces the bioassay uncertainty. A low coefficient of 
      variation (CV) of 3.3% confirms the high reproducibility of the disposable 
      HOF-OFL sensors, and the mean of the normal distribution of the logarithmic OFL 
      intensity serves as the sensing output. A limit of detection of 11 nM within a 
      short assay time of 15 min is achieved. These disposable immunosensors possess 
      the advantages of low cost, high reproducibility, fast assay, and low-volume 
      consumption of sample and reagents. We believe that this work will inspire 
      disposable optofluidics through the mass production of multifunctional 
      microstructured optical fibers.
FAU - Yang, Xi
AU  - Yang X
AD  - Key Laboratory of Optical Fiber Sensing and Communications (Ministry of Education 
      of China), University of Electronic Science and Technology of China, No. 2006, 
      Xiyuan Ave., Chengdu, 611731, China. ygong@uestc.edu.cn.
FAU - Luo, Yanhua
AU  - Luo Y
AD  - School of Electrical Engineering and Telecommunications, University of New South 
      Wales, Sydney, NSW 2052, Australia. g.peng@unsw.edu.au.
FAU - Liu, Yiling
AU  - Liu Y
AD  - Key Laboratory of Optical Fiber Sensing and Communications (Ministry of Education 
      of China), University of Electronic Science and Technology of China, No. 2006, 
      Xiyuan Ave., Chengdu, 611731, China. ygong@uestc.edu.cn.
FAU - Gong, Chaoyang
AU  - Gong C
AD  - Key Laboratory of Optical Fiber Sensing and Communications (Ministry of Education 
      of China), University of Electronic Science and Technology of China, No. 2006, 
      Xiyuan Ave., Chengdu, 611731, China. ygong@uestc.edu.cn and School of Electrical 
      and Electronics Engineering, Nanyang Technological University, 50 Nanyang Ave, 
      Singapore, 639798, Singapore.
FAU - Wang, Yanqiong
AU  - Wang Y
AD  - Key Laboratory of Optical Fiber Sensing and Communications (Ministry of Education 
      of China), University of Electronic Science and Technology of China, No. 2006, 
      Xiyuan Ave., Chengdu, 611731, China. ygong@uestc.edu.cn.
FAU - Rao, Yun-Jiang
AU  - Rao YJ
AD  - Key Laboratory of Optical Fiber Sensing and Communications (Ministry of Education 
      of China), University of Electronic Science and Technology of China, No. 2006, 
      Xiyuan Ave., Chengdu, 611731, China. ygong@uestc.edu.cn.
FAU - Peng, Gang-Ding
AU  - Peng GD
AD  - School of Electrical Engineering and Telecommunications, University of New South 
      Wales, Sydney, NSW 2052, Australia. g.peng@unsw.edu.au.
FAU - Gong, Yuan
AU  - Gong Y
AUID- ORCID: 0000-0001-6819-8402
AD  - Key Laboratory of Optical Fiber Sensing and Communications (Ministry of Education 
      of China), University of Electronic Science and Technology of China, No. 2006, 
      Xiyuan Ave., Chengdu, 611731, China. ygong@uestc.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Lab Chip
JT  - Lab on a chip
JID - 101128948
SB  - IM
MH  - *Biosensing Techniques
MH  - Immunoassay
MH  - Lasers
MH  - *Optical Fibers
MH  - Reproducibility of Results
EDAT- 2020/02/06 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/02/06 06:00
PHST- 2020/02/06 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/02/06 06:00 [entrez]
AID - 10.1039/c9lc01216h [doi]
PST - ppublish
SO  - Lab Chip. 2020 Mar 3;20(5):923-930. doi: 10.1039/c9lc01216h.