PMID- 32023024
OWN - NLM
STAT- MEDLINE
DCOM- 20201211
LR  - 20201214
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Linking)
VI  - 12
IP  - 9
DP  - 2020 Mar 4
TI  - Optimization of Sporulation and Germination Conditions of Functional Bacteria for 
      Concrete Crack-Healing and Evaluation of their Repair Capacity.
PG  - 10938-10948
LID - 10.1021/acsami.9b21465 [doi]
AB  - While microbial spore production and germination of bacteria have been widely 
      studied for their applications in animal husbandry, aquatic products, medicine, 
      and food, few studies have investigated their use for the crack-healing of 
      concrete. To effectively heal the cracks in concrete, studies suggest that the 
      rate of sporulation and the germination of bacteria should be sufficiently high. 
      This study investigates the effects of different carbon sources, nitrogen 
      sources, Mn(2+) concentrations, and external culture conditions on the 
      sporulation rate and analyzes the effects of the pH value, heat activation, 
      germinants, various cations, and nutrients on the germination of spores. Bacillus 
      cohnii (B. cohnii) is chosen as the bacterium to be mixed in concrete because of 
      its alkalophilic nature. The mineralization activity of spores after germination 
      and the crack-healing capacity of concrete are studied. The optimal culture 
      medium and the optimum external conditions for spore production are obtained. The 
      total cell count and sporulation rate of bacteria obtained on this medium are 
      3.14 x 10(9) CFU/mL and 92.6%, respectively, under the optimum external 
      conditions. The optimal pH value for the spore germination of B. cohnii is 9.7. 
      While the cation Na(+) strongly stimulates the germination of B. cohnii spores, 
      other cations (such as K(+), NH(4)(+), and Ca(2+)) do not stimulate spore 
      germination. The optimal concentration of Na(+) is 200 mM. The germination rate 
      of spores in the control group concrete specimen (room temperature 24 degrees C) was more 
      than 50%, thus suggesting that B. cohnii bacteria can be used in the self-healing 
      of concrete cracks. The mineralization activity test proves that the spores of B. 
      cohnii have a mineralizing function after germination, and the crystals produced 
      by microbial-induced carbonate precipitation (MICP) are of pure calcite. When the 
      crack width of the concrete specimen with spores of B. cohnii is less than 1.2 
      mm, it can be completely repaired after 28 days of healing.
FAU - Jiang, Lu
AU  - Jiang L
AD  - College of Civil Engineering, Taiyuan University of Technology, Taiyuan, Shanxi 
      030024, P. R. China.
AD  - Shanxi Shengke Microorganism Building Materials Technology Co., Ltd., Taiyuan, 
      Shanxi 030032, P. R. China.
FAU - Jia, Guanhua
AU  - Jia G
AD  - Department of Civil Engineering, Shanxi University, Tauyuan, Shanxi 030013, P. R. 
      China.
AD  - Shanxi Shengke Microorganism Building Materials Technology Co., Ltd., Taiyuan, 
      Shanxi 030032, P. R. China.
FAU - Wang, Yongzhen
AU  - Wang Y
AD  - College of Materials Science and Engineering, Taiyuan University of Technology, 
      Taiyuan, Shanxi 030024, P. R. China.
FAU - Li, Zhu
AU  - Li Z
AUID- ORCID: 0000-0002-7044-0752
AD  - College of Civil Engineering, Taiyuan University of Technology, Taiyuan, Shanxi 
      030024, P. R. China.
AD  - Shanxi Shengke Microorganism Building Materials Technology Co., Ltd., Taiyuan, 
      Shanxi 030032, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20200225
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Carbonates)
RN  - 0 (Culture Media)
RN  - H0G9379FGK (Calcium Carbonate)
SB  - IM
MH  - Bacillus/chemistry/*growth & development/metabolism
MH  - Calcium Carbonate/metabolism
MH  - Carbonates/metabolism
MH  - Construction Materials/analysis/*microbiology
MH  - Culture Media/chemistry/metabolism
MH  - Hydrogen-Ion Concentration
MH  - Spores, Bacterial/chemistry/*growth & development/metabolism
OTO - NOTNLM
OT  - MICP
OT  - concrete
OT  - crack-healing
OT  - germination
OT  - sporulation rate
EDAT- 2020/02/06 06:00
MHDA- 2020/12/15 06:00
CRDT- 2020/02/06 06:00
PHST- 2020/02/06 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/02/06 06:00 [entrez]
AID - 10.1021/acsami.9b21465 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2020 Mar 4;12(9):10938-10948. doi: 
      10.1021/acsami.9b21465. Epub 2020 Feb 25.