PMID- 32023616
OWN - NLM
STAT- MEDLINE
DCOM- 20201015
LR  - 20201015
IS  - 1423-0291 (Electronic)
IS  - 1015-2008 (Linking)
VI  - 87
IP  - 1
DP  - 2020
TI  - Development of a Novel One-Step Automated Rapid in situ Hybridization for 
      Anaplastic Lymphoma Kinase Rearrangement Using Non-Contact Alternating-Current 
      Electric-Field Mixing.
PG  - 45-50
LID - 10.1159/000505631 [doi]
AB  - Echinoderm microtubule-associated protein-like 4 and anaplastic lymphoma kinase 
      (ALK) fusion gene rearrangement is a key driver mutation in non-small cell lung 
      cancer (NSCLC). Although Break-Apart ALK fluorescence in situ hybridization 
      (FISH) is a reliable diagnostic method for detecting ALK gene rearrangement, it 
      is also costly and time-consuming to use as a routine screening test. Our aim was 
      to evaluate the clinical utility of a novel one-step, automated, rapid FISH 
      (Auto-RaFISH) method developed to facilitate hybridization. This method takes 
      advantage of the non-contact mixing effect of an alternating-current electric 
      field. Ten representative specimens from 85 patients diagnosed at multiple 
      centers with primary lung cancer with identified ALK-FISH status were collected. 
      The specimens were all tested using FISH, RaFISH, and Auto-RaFISH. With both 
      RaFISH protocols, the ALK test was completed within 4.5 h, as compared to the 20 
      h needed for the standard protocol. We found 100% agreement between the standard 
      FISH, RaFISH, and new Auto-RaFISH based on the ALK status, and all methods 
      stained equally well. These findings suggest that Auto-RaFISH could potentially 
      serve as an automated clinical tool for prompt determination of ALK status in 
      NSCLC.
CI  - (c) 2020 S. Karger AG, Basel.
FAU - Imai, Kazuhiro
AU  - Imai K
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan, i-karo@mui.biglobe.ne.jp.
FAU - Takashima, Shinogu
AU  - Takashima S
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Fujishima, Satoshi
AU  - Fujishima S
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Matsuo, Tsubasa
AU  - Matsuo T
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Watanabe, Shin-Nosuke
AU  - Watanabe SN
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Nanjo, Hiroshi
AU  - Nanjo H
AD  - Department of Pathology,Akita University Hospital, Akita, Japan.
FAU - Akagami, Yoichi
AU  - Akagami Y
AD  - Akita Prefectural Research and Development Center, Akita, Japan.
FAU - Nakamura, Ryuta
AU  - Nakamura R
AD  - Akita Prefectural Research and Development Center, Akita, Japan.
FAU - Terata, Kaori
AU  - Terata K
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Wakita, Akiyuki
AU  - Wakita A
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Sato, Yusuke
AU  - Sato Y
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Motoyama, Satoru
AU  - Motoyama S
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
FAU - Minamiya, Yoshihiro
AU  - Minamiya Y
AD  - Department of Thoracic Surgery, Akita University Graduate School of Medicine, 
      Akita, Japan.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20200205
PL  - Switzerland
TA  - Pathobiology
JT  - Pathobiology : journal of immunopathology, molecular and cellular biology
JID - 9007504
RN  - EC 2.7.10.1 (ALK protein, human)
RN  - EC 2.7.10.1 (Anaplastic Lymphoma Kinase)
SB  - IM
MH  - Anaplastic Lymphoma Kinase/*genetics
MH  - Automation, Laboratory
MH  - Carcinoma, Non-Small-Cell Lung/*genetics
MH  - *Electricity
MH  - *Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mutation
OTO - NOTNLM
OT  - Anaplastic lymphoma kinase
OT  - In situ hybridization
OT  - Lung cancer
OT  - Non-contact alternating-current electric-field mixing
EDAT- 2020/02/06 06:00
MHDA- 2020/10/21 06:00
CRDT- 2020/02/06 06:00
PHST- 2019/11/15 00:00 [received]
PHST- 2019/12/29 00:00 [accepted]
PHST- 2020/02/06 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
PHST- 2020/02/06 06:00 [entrez]
AID - 000505631 [pii]
AID - 10.1159/000505631 [doi]
PST - ppublish
SO  - Pathobiology. 2020;87(1):45-50. doi: 10.1159/000505631. Epub 2020 Feb 5.