PMID- 32027612
OWN - NLM
STAT- MEDLINE
DCOM- 20200406
LR  - 20200408
IS  - 1972-2680 (Electronic)
IS  - 1972-2680 (Linking)
VI  - 12
IP  - 12
DP  - 2018 Dec 31
TI  - HLA-C*18:01 and KIR2DL2+C1 genetic variants are associated with low viral load in 
      cART naive HIV-infected adult Zimbabweans.
PG  - 1105-1111
LID - 10.3855/jidc.10848 [doi]
AB  - INTRODUCTION: Polymorphisms in killer cell immunoglobulin-like receptor (KIR) and 
      human leukocyte antigen (HLA) gene families are implicated in differential 
      outcomes of HIV infection. However, research findings on the influence of KIR and 
      HLA-C polymorphism on HIV disease progression remain inconclusive. We thus 
      investigated the association of KIR and HLA-C gene polymorphisms with plasma HIV 
      load (VL) and CD4+ T lymphocyte (CD4) count in 183 chronically HIV-infected, 
      combination antiretroviral therapy (cART) naive Zimbabweans of Bantu origin. 
      METHODOLOGY: The presence or absence of 15 KIR genes were determined using 
      sequence specific primer polymerase chain reaction while HLA-C typing was 
      performed using chain termination DNA sequencing. Plasma VL was determined using 
      the Cavidi Exavir viral load version 3 assay while CD4+ T lymphocytes were 
      enumerated using flow cytometry. VLs and CD4 counts were compared between 
      gene/genotype carriers and non-carriers using Mann-Whitney ranksum test. RESULTS: 
      HLA-C*18:01 allele carriers had a significantly lower median log10 VL 
      (2.87copies/mL [IQR;2.3-3.2]) than the non-C*18:01 carriers (3.33copies/mL [IQR; 
      2.74-3.9]), p = 0.018. Further, median log10 VL was significantly lower in 
      KIR2DL2+C1 carriers (2.745 [IQR; 2.590-2.745]) than non-KIR2DL2+C1 carriers (3.4 
      [IQR; 2.746-3.412]), p = 0.041. Comparison of CD4 + T lymphocyte counts between 
      C*08:02 allele carriers and non-C*08:02 carriers showed a significantly higher 
      median CD4 count in C*08:02 carriers (548cells/microL [IQR;410-684]) than in 
      non-carriers (428cells/microL [IQR;388-537]), p = 0.034. CONCLUSION: We conclude that 
      the HLA-C*18:01 and KIR2DL2+C1 genetic variants are associated with low VL while 
      the C*08:02 is associated with high CD4+ T lymphocyte count among cART naive 
      Zimbabwean adults with chronic HIV infection.
CI  - Copyright (c) 2018 Kudakwashe Mhandire, Mqondisi Tshabalala, Lynn Sodai Zijenah, 
      Sarah L Rowland-Jones, Babill Stray-Pedersen.
FAU - Mhandire, Kudakwashe
AU  - Mhandire K
AD  - University of Zimbabwe College of Health Sciences, Harare, Zimbabwe. 
      mhandiremhandire@gmail.com.
FAU - Tshabalala, Mqondisi
AU  - Tshabalala M
AD  - University of Zimbabwe College of Health Sciences, Harare, Zimbabwe. 
      mtshabaz@gmail.com.
FAU - Zijenah, Lynn Sodai
AU  - Zijenah LS
AD  - University of Zimbabwe College of Health Sciences, Harare, Zimbabwe. 
      lzijenah@gmail.com.
FAU - Mlambo, Tommy
AU  - Mlambo T
AD  - University of Zimbabwe College of Health Sciences, Harare, Zimbabwe. 
      address@notprovi.ded.
FAU - Mhandire, Doreen Zvipo
AU  - Mhandire DZ
AD  - University of Cape Town, Cape Town, South Africa. address@notprovi.ded.
FAU - Musarurwa, Cuthbert
AU  - Musarurwa C
AD  - University of Zimbabwe College of Health Sciences, Harare, Zimbabwe. 
      address@notprovi.ded.
FAU - Duri, Kerina
AU  - Duri K
AD  - University of Zimbabwe College of Health Sciences, Harare, Zimbabwe. 
      address@notprovi.ded.
FAU - Matarira, Hilda Tendisa
AU  - Matarira HT
AD  - University of Zimbabwe College of Health Sciences, Harare, Zimbabwe. 
      address@notprovi.ded.
FAU - Dandara, Collet
AU  - Dandara C
AD  - University of Cape Town, Cape Town, South Africa. address@notprovi.ded.
FAU - Rowland-Jones, Sarah L
AU  - Rowland-Jones SL
AD  - University of Oxford, Oxford, United Kingdom. sarah.rowland-jones@ndm.ox.ac.uk.
FAU - Stray-Pedersen, Babill
AU  - Stray-Pedersen B
AD  - University Hospital, Oslo, Norway. babill.stray-pedersen@medisin.uio.no.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20181231
PL  - Italy
TA  - J Infect Dev Ctries
JT  - Journal of infection in developing countries
JID - 101305410
RN  - 0 (HLA-C Antigens)
RN  - 0 (KIR2DL2 protein, human)
RN  - 0 (Receptors, KIR2DL2)
SB  - IM
MH  - Adult
MH  - Antiretroviral Therapy, Highly Active
MH  - CD4-Positive T-Lymphocytes/physiology/virology
MH  - Cross-Sectional Studies
MH  - Gene Frequency
MH  - HIV Infections/drug therapy/*genetics/*virology
MH  - HLA-C Antigens/*genetics
MH  - Humans
MH  - Polymorphism, Genetic
MH  - Receptors, KIR2DL2/*genetics
MH  - Viral Load
MH  - Zimbabwe
OTO - NOTNLM
OT  - HIV
OT  - HLA
OT  - KIR
OT  - Zimbabweans
OT  - natural killer cells
OT  - viral load
COIS- No Conflict of Interest is declared
EDAT- 2018/12/31 00:00
MHDA- 2020/04/09 06:00
CRDT- 2020/02/07 06:00
PHST- 2018/09/10 00:00 [received]
PHST- 2018/11/20 00:00 [accepted]
PHST- 2020/02/07 06:00 [entrez]
PHST- 2018/12/31 00:00 [pubmed]
PHST- 2020/04/09 06:00 [medline]
AID - 10.3855/jidc.10848 [doi]
PST - epublish
SO  - J Infect Dev Ctries. 2018 Dec 31;12(12):1105-1111. doi: 10.3855/jidc.10848.