PMID- 32028263
OWN - NLM
STAT- MEDLINE
DCOM- 20210122
LR  - 20210122
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 12
IP  - 3
DP  - 2020 Feb 6
TI  - Methionine increases yolk production to offset the negative effect of caloric 
      restriction on reproduction without affecting longevity in C. elegans.
PG  - 2680-2697
LID - 10.18632/aging.102770 [doi]
AB  - Caloric restriction (CR) or Dietary restriction (DR) is known to improve health 
      and in many cases increases lifespan. However, its negative effect on 
      reproduction has not been fully studied. Practicing CR/DR without adequate 
      knowledge on its side effect may risk complications such as infertility, birth 
      defect, or malnutrition. In this study, by using several CR strategies in C. 
      elegans, we examine key functions of reproduction including embryonic development 
      and larvae growth. We find that CR significantly decreases the survival of 
      embryos and slows the growth of the offspring. We further determine that defect 
      in oocyte but not sperm is responsible for the compromised reproduction under CR. 
      Interestingly, adding methionine to the medium reverses the reproduction defects, 
      but does not affect the long lifespan resulted from CR. The beneficial effect of 
      methionine on reproduction requires the yolk protein vitellogenin. CR 
      down-regulates vitellogenin expression, which can be reversed by supplementing 
      methionine in the food. Lacking the yolk protein transport due to rme-2 mutation 
      blocks methionine's beneficial effects. Our study has revealed a novel, 
      methionine-mediated genetic pathway linking nutrient sensing to reproduction and 
      suggested methionine as a potential food supplement to mitigate the side effect 
      of CR.
FAU - Zhou, Gaojian
AU  - Zhou G
AD  - Basic Medicine College, Central South University, Changsha, Hunan, People's 
      Republic of China.
FAU - Huang, Chuan
AU  - Huang C
AD  - Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, People's 
      Republic of China.
FAU - Xing, Liu
AU  - Xing L
AD  - Reproductive and Genetic Hospital of CITIC-Xiangya, Changsha, Hunan, People's 
      Republic of China.
FAU - Li, Le
AU  - Li L
AD  - ProMab Biotechnologies, Richmond, CA 94806, USA.
FAU - Jiang, Yan
AU  - Jiang Y
AD  - Hunan Gaocell Medical Technology Co. Ltd., Changsha, Hunan, People's Republic of 
      China.
FAU - Wei, Yuehua
AU  - Wei Y
AD  - Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of 
      Medicine, Shanghai, People's Republic of China.
LA  - eng
GR  - P40 OD010440/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200206
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - Caenorhabditis elegans/*physiology
MH  - *Dietary Supplements
MH  - Egg Yolk/*metabolism
MH  - *Energy Intake
MH  - *Longevity
MH  - Methionine/administration & dosage/*pharmacology
PMC - PMC7041781
OTO - NOTNLM
OT  - calorie restriction (CR)
OT  - methionine
OT  - reproduction
OT  - side effect
OT  - vitellogenin
COIS- CONFLICTS OF INTEREST: The authors declare no conflicts of interest.
EDAT- 2020/02/07 06:00
MHDA- 2021/01/23 06:00
PMCR- 2020/02/15
CRDT- 2020/02/07 06:00
PHST- 2019/10/24 00:00 [received]
PHST- 2020/01/12 00:00 [accepted]
PHST- 2020/02/07 06:00 [pubmed]
PHST- 2021/01/23 06:00 [medline]
PHST- 2020/02/07 06:00 [entrez]
PHST- 2020/02/15 00:00 [pmc-release]
AID - 102770 [pii]
AID - 10.18632/aging.102770 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2020 Feb 6;12(3):2680-2697. doi: 10.18632/aging.102770. Epub 
      2020 Feb 6.