PMID- 32028606
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2077-0383 (Print)
IS  - 2077-0383 (Electronic)
IS  - 2077-0383 (Linking)
VI  - 9
IP  - 2
DP  - 2020 Feb 3
TI  - Involvement of the Endocrine-Disrupting Chemical Bisphenol A (BPA) in Human 
      Placentation.
LID - 10.3390/jcm9020405 [doi]
LID - 405
AB  - BACKGROUND: Endocrine-disrupting chemicals (EDCs) are environmental 
      chemicals/toxicants that humans are exposed to, interfering with the action of 
      multiple hormones. Bisphenol A (BPA) is classified as an EDC with xenoestrogenic 
      activity with potentially adverse effects in reproduction. Currently, a 
      significant knowledge gap remains regarding the complete spectrum of BPA-induced 
      effects on the human placenta. As such, the present study examined the effects of 
      physiologically relevant doses of BPA in vitro. METHODS: qRT-PCR, Western 
      blotting, immunofluorescence, ELISA, microarray analyses, and bioinformatics have 
      been employed to study the effects of BPA using nonsyncytialised (non-ST) and 
      syncytialised (ST) BeWo cells. RESULTS: Treatment with 3 nM BPA led to an 
      increase in cell number and altered the phosphorylation status of p38, an effect 
      mediated primarily via the membrane-bound estrogen receptor (GPR30). Nonbiased 
      microarray analysis identified 1195 and 477 genes that were differentially 
      regulated in non-ST BeWo cells, whereas in ST BeWo cells, 309 and 158 genes had 
      altered expression when treated with 3 and 10 nM, respectively. Enriched pathway 
      analyses in non-ST BeWo identified a leptin and insulin overlap (3 nM), 
      methylation pathways (10 nM), and differentiation of white and brown adipocytes 
      (common). In the ST model, most significantly enriched were the nuclear factor 
      erythroid 2-related factor 2 (NRF2) pathway (3 nM) and mir-124 predicted 
      interactions with cell cycle and differentiation (10 nM). CONCLUSION: 
      Collectively, our data offer a new insight regarding BPA effects at the placental 
      level, and provide a potential link with metabolic changes that can have an 
      impact on the developing fetus.
FAU - de Aguiar Greca, Sophie-Christine
AU  - de Aguiar Greca SC
AD  - College of Health and Life Sciences, Brunel University London, Uxbridge UB8 3PH, 
      UK.
FAU - Kyrou, Ioannis
AU  - Kyrou I
AD  - Aston Medical Research Institute, Aston Medical School, Aston University, 
      Birmingham B4 7ET, UK.
AD  - Warwickshire Institute for the Study of Diabetes, Endocrinology and Metabolism 
      (WISDEM), University Hospitals Coventry and Warwickshire NHS Trust, Coventry CV2 
      2DX, UK.
AD  - Institute of Precision Diagnostics and Translational Medicine, UHCW NHS Trust, 
      Coventry CV4 7AL, UK.
AD  - Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
FAU - Pink, Ryan
AU  - Pink R
AD  - Dept of Bio. & Med. Sci., Oxford Brookes University, Oxford OX3 0BP, UK.
FAU - Randeva, Harpal
AU  - Randeva H
AD  - Institute of Precision Diagnostics and Translational Medicine, UHCW NHS Trust, 
      Coventry CV4 7AL, UK.
AD  - Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
FAU - Grammatopoulos, Dimitris
AU  - Grammatopoulos D
AD  - Institute of Precision Diagnostics and Translational Medicine, UHCW NHS Trust, 
      Coventry CV4 7AL, UK.
AD  - Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK.
FAU - Silva, Elisabete
AU  - Silva E
AD  - College of Health and Life Sciences, Brunel University London, Uxbridge UB8 3PH, 
      UK.
FAU - Karteris, Emmanouil
AU  - Karteris E
AD  - College of Health and Life Sciences, Brunel University London, Uxbridge UB8 3PH, 
      UK.
LA  - eng
PT  - Journal Article
DEP - 20200203
PL  - Switzerland
TA  - J Clin Med
JT  - Journal of clinical medicine
JID - 101606588
PMC - PMC7074564
OTO - NOTNLM
OT  - BPA
OT  - endocrine-disrupting chemicals
OT  - microarray
OT  - placenta
COIS- The authors declare no conflict of interest.
EDAT- 2020/02/08 06:00
MHDA- 2020/02/08 06:01
PMCR- 2020/02/03
CRDT- 2020/02/08 06:00
PHST- 2019/12/26 00:00 [received]
PHST- 2020/01/22 00:00 [revised]
PHST- 2020/01/23 00:00 [accepted]
PHST- 2020/02/08 06:00 [entrez]
PHST- 2020/02/08 06:00 [pubmed]
PHST- 2020/02/08 06:01 [medline]
PHST- 2020/02/03 00:00 [pmc-release]
AID - jcm9020405 [pii]
AID - jcm-09-00405 [pii]
AID - 10.3390/jcm9020405 [doi]
PST - epublish
SO  - J Clin Med. 2020 Feb 3;9(2):405. doi: 10.3390/jcm9020405.