PMID- 32028827
OWN - NLM
STAT- MEDLINE
DCOM- 20210601
LR  - 20210601
IS  - 1933-6926 (Electronic)
IS  - 1933-6918 (Print)
IS  - 1933-6918 (Linking)
VI  - 14
IP  - 1
DP  - 2020 Dec
TI  - Thymol alleviates AGEs-induced podocyte injury by a pleiotropic effect via 
      NF-kappaB-mediated by RhoA/ROCK signalling pathway.
PG  - 42-56
LID - 10.1080/19336918.2020.1721172 [doi]
AB  - Advanced glycation end products (AGE) are those of the most powerful pathogenic 
      factors that related to diabetic complications. In our study, we investigated the 
      beneficial effects of thymol on AGE induced cell injury and apoptosis in human 
      podocytes (HPCs) and attempted to clarify its mechanisms. Our results revealed 
      that stimulation with AGE could significantly activate RhoA/NF-kappaB pathway. 
      Results showed thymol could markedly suppress inflammatory responses, cell 
      apoptosis and disordered cytoskeleton. Also thymol restored the expression of 
      podocin, restrained migration capacity. Western blot analysis indicated that it 
      could restore the expression of RhoA, ROCK and vimentin, nephrin, podocin and p65 
      and IkappaBalpha phosphorylation. Moreover, si-RhoA also suppressed the expression of 
      pro-inflammatory cytokines, ROCK, and vimentin and the phosphorylation of p65 and 
      IkappaBalpha. In conclusion, thymol inhibits AGE-induced cell injury in HPCs by 
      suppressing the RhoA-NF-kappaB pathway and may be apromising therapeutic agent.
FAU - Wang, Qinglian
AU  - Wang Q
AD  - Department of Nephrology, Shandong Provincial Hospital Affiliated to Shandong 
      University, Jinan, China.
FAU - Shen, Zhenwei
AU  - Shen Z
AD  - Department of Biostatistics, WuXi Clinical Development Service (Shanghai) Co., 
      Ltd, Shanghai, China.
FAU - Qi, Guanghui
AU  - Qi G
AD  - Department of Urological Surgery, The First Hospital of Zibo, Shandong, China.
FAU - Zhao, Yanfang
AU  - Zhao Y
AD  - Department of Nephrology, Shandong Provincial Hospital Affiliated to Shandong 
      University, Jinan, China.
FAU - Zhang, Hongge
AU  - Zhang H
AD  - Department of Urological Surgery, Tengzhou Hospital of Traditional Chinese 
      Medicine, Zaozhuang, China.
FAU - Wang, Rong
AU  - Wang R
AD  - Department of Nephrology, Shandong Provincial Hospital Affiliated to Shandong 
      University, Jinan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cell Adh Migr
JT  - Cell adhesion & migration
JID - 101469464
RN  - 0 (Cytokines)
RN  - 0 (Glycation End Products, Advanced)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (NF-kappa B)
RN  - 0 (NPHS2 protein)
RN  - 0 (Protective Agents)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Vimentin)
RN  - 0 (nephrin)
RN  - 3J50XA376E (Thymol)
RN  - EC 2.7.11.1 (rho-Associated Kinases)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - Cell Line
MH  - Cell Movement/drug effects
MH  - Cell Survival/drug effects
MH  - Cytokines/metabolism
MH  - Cytoskeleton/metabolism
MH  - Glycation End Products, Advanced/*toxicity
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - Membrane Proteins/metabolism
MH  - NF-kappa B/*metabolism
MH  - Podocytes/*pathology
MH  - Protective Agents/pharmacology
MH  - RNA, Small Interfering/metabolism
MH  - *Signal Transduction/drug effects
MH  - Thymol/chemistry/*pharmacology
MH  - Vimentin/metabolism
MH  - rho-Associated Kinases/*metabolism
MH  - rhoA GTP-Binding Protein/*metabolism
PMC - PMC7757830
OTO - NOTNLM
OT  - RhoA/ROCK pathway
OT  - Thymol
OT  - advanced glycation end products (AGE)
OT  - human podocytes
OT  - nuclear factor-kappa B (NF-kappaB)
EDAT- 2020/02/08 06:00
MHDA- 2021/06/02 06:00
PMCR- 2020/02/07
CRDT- 2020/02/08 06:00
PHST- 2020/02/08 06:00 [entrez]
PHST- 2020/02/08 06:00 [pubmed]
PHST- 2021/06/02 06:00 [medline]
PHST- 2020/02/07 00:00 [pmc-release]
AID - 1721172 [pii]
AID - 10.1080/19336918.2020.1721172 [doi]
PST - ppublish
SO  - Cell Adh Migr. 2020 Dec;14(1):42-56. doi: 10.1080/19336918.2020.1721172.