PMID- 32029267
OWN - NLM
STAT- MEDLINE
DCOM- 20200624
LR  - 20200624
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1618
DP  - 2020 May 10
TI  - Aqueous extraction followed by dispersive solid phase extraction with in situ 
      derivatization for the determination of aflatoxins in traditional Chinese 
      medicines.
PG  - 460894
LID - S0021-9673(20)30071-6 [pii]
LID - 10.1016/j.chroma.2020.460894 [doi]
AB  - A green sample preparation method based on aqueous extraction followed by 
      dispersive solid phase extraction (d-SPE) with in situ derivatization (ISD) was 
      developed for the determination of aflatoxins (AFs) in traditional Chinese 
      medicines (TCMs). AFs in TCMs were extracted by alkaline aqueous solution and 
      converted to substituted coumaric acids. Then, mixed-mode anion exchange (MAX) 
      sorbent was used to isolate and enrich the substituted coumaric acids. During the 
      elution by acetonitrile/trifluoroacetic acid solution, AFs were reconstructed and 
      in situ derivatized. Several parameters affecting the procedure were evaluated. 
      The developed preparation method coupled with high performance liquid 
      chromatography-fluorescence detection was successfully applied for AFs 
      determination in TCMs. The limit of detection (LOD) reached 10 pg/mL for AFs. 
      Good linearity was obtained in three orders of magnitude with correlation 
      coefficients ranging from 0.9996 to 0.9999. The relative recoveries of the method 
      were between 72.7% and 114.5% with intra- and inter-day relative standard 
      deviations (RSDs) less than 9.5% and 10.1%, respectively. The method was 
      successfully applied to determine AFs in 15 kinds of TCMs in China, with the 
      results verified by IAC standard method.
CI  - Copyright (c) 2020. Published by Elsevier B.V.
FAU - Wang, Nan
AU  - Wang N
AD  - CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Department of 
      Instrumentation & Analytical Chemistry, Dalian Institute of Chemical Physics, 
      Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China; 
      University of Chinese Academy of Sciences, Beijing 100039, PR China.
FAU - Duan, Chunfeng
AU  - Duan C
AD  - CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Department of 
      Instrumentation & Analytical Chemistry, Dalian Institute of Chemical Physics, 
      Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China.
FAU - Li, Shenghong
AU  - Li S
AD  - CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Department of 
      Instrumentation & Analytical Chemistry, Dalian Institute of Chemical Physics, 
      Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China.
FAU - Geng, Xuhui
AU  - Geng X
AD  - CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Department of 
      Instrumentation & Analytical Chemistry, Dalian Institute of Chemical Physics, 
      Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China.
FAU - Ding, Kun
AU  - Ding K
AD  - CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Department of 
      Instrumentation & Analytical Chemistry, Dalian Institute of Chemical Physics, 
      Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China.
FAU - Guan, Yafeng
AU  - Guan Y
AD  - CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Department of 
      Instrumentation & Analytical Chemistry, Dalian Institute of Chemical Physics, 
      Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, PR China. 
      Electronic address: guanyafeng@dicp.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20200115
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Aflatoxins)
RN  - 059QF0KO0R (Water)
SB  - IM
MH  - Aflatoxins/*analysis
MH  - Chemistry, Pharmaceutical/*methods
MH  - China
MH  - Chromatography, High Pressure Liquid
MH  - Limit of Detection
MH  - *Medicine, Chinese Traditional
MH  - *Solid Phase Extraction
MH  - Water/*chemistry
OTO - NOTNLM
OT  - Aflatoxins
OT  - Dispersive solid phase extraction
OT  - Fluorescence
OT  - In situ derivatization
OT  - Ionic interaction
OT  - Traditional Chinese medicines
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/02/08 06:00
MHDA- 2020/06/25 06:00
CRDT- 2020/02/08 06:00
PHST- 2019/08/20 00:00 [received]
PHST- 2019/12/12 00:00 [revised]
PHST- 2020/01/14 00:00 [accepted]
PHST- 2020/02/08 06:00 [pubmed]
PHST- 2020/06/25 06:00 [medline]
PHST- 2020/02/08 06:00 [entrez]
AID - S0021-9673(20)30071-6 [pii]
AID - 10.1016/j.chroma.2020.460894 [doi]
PST - ppublish
SO  - J Chromatogr A. 2020 May 10;1618:460894. doi: 10.1016/j.chroma.2020.460894. Epub 
      2020 Jan 15.