PMID- 32030953
OWN - NLM
STAT- MEDLINE
DCOM- 20200228
LR  - 20220412
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 34
IP  - 2
DP  - 2020 Feb 15
TI  - [Effect of icariin on early steroid-induced osteonecrosis of the femoral head in 
      rabbits].
PG  - 206-212
LID - 10.7507/1002-1892.201905112 [doi]
AB  - OBJECTIVE: To explore the effect of icariin on early steroid-induced 
      osteonecrosis of the femoral head in rabbits. METHODS: Fifty mature New Zealand 
      rabbits (weighing, 2.5-3.0 kg) were randomly divided into control group ( n=10), 
      model group ( n=20), and experimental group ( n=20). The rabbits of model and 
      experimental groups were injected with lipopolysaccharide and methylprednisolone 
      to establish the animal model of early steroid-induced osteonecrosis of the 
      femoral head. The rabbits of experimental group were feeded with icariin solution 
      once a day for 6 weeks since the first injection of methylprednisolone, whereas 
      the rabbits of control and model groups were given normal saline at the same time 
      points. The left femoral heads were removed after 6 weeks and gross morphological 
      features were evaluated. Micro-CT scan was performed to analyze the trabecular 
      microstructure with the following parameters: trabecular bone volume to total 
      volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Tn), and 
      trabecular separation (Tb.Sp). The Micro-CT scan was also converted to 
      three-dimensional reconstruction images for observation. HE staining was applied 
      to observe the trabecular structure and morphological changes of osteocytes and 
      marrow adipocytes. It was also used to determine whether the samples of femoral 
      heads occurred osteonecrosis based on the criteria for pathological diagnosis, 
      and calculate the rate of empty lacunae. RESULTS: Seven rabbits died during the 
      study, and 9, 16, and 18 rabbits in the control, model, and experimental groups, 
      respectively, enrolled the final analysis. Compared with control group, the 
      femoral head collapse and trabecular breaks were more obvious, and the trabeculae 
      were sparse with irregular arrangement in the model group according to the 
      results of gross observation, Micro-CT scan, and three-dimensional reconstruction 
      images. But in the experimental group, the surface of femoral head was slight 
      shrinking without obvious collapse, and the degeneration of trabecular structure 
      was mild. According to bone microstructures analysis, the Tb.N, Tb.Tn, and BV/TV 
      of femoral head in model and experimental groups were lower than those in control 
      group, while the Tb.Sp in the model and experimental groups were significantly 
      higher. The Tb.N, Tb.Tn, and BV/TV of femoral head in experimental group were 
      higher than those in model group, while the Tb.Sp in the experimental group was 
      significantly lower. The differences between groups were all significant ( 
      P<0.05). In the model group, HE staining showed that the number of osteocytes 
      reduced, the number of empty lacunae increased, and the marrow adipocytes piled 
      up in the space between femoral trabeculae, some even mashed together like a 
      cyst. In the experimental group, the trabecular structure was still relatively 
      complete compared with model group, no obvious apoptosis of osteocytes was 
      observed, the size and number of adipocytes were basically normal. None of the 
      animals in control group occurred osteonecrosis of the femoral head based on the 
      criteria for pathological diagnosis, and the incidence of osteonecrosis were 
      81.3% (13/16) in the model group and 66.7% (12/18) in the experimental group, and 
      the difference was not significant ( P=0.448). The rate of empty lacunae of 
      osteonecrotic femoral heads in the model group was 33.1%+/-1.4%, which was higher 
      than that in experimental group (18.9%+/-0.8%) and in control group (12.7%+/-1.5%), 
      and the differences between groups were significant ( P<0.05). CONCLUSION: The 
      icariin has a protective effect on the early steroid-induced osteonecrosis of the 
      femoral head in rabbits, which can decrease osteocytes apoptosis, improve the 
      bone microstructure, and delay such disease processes.
FAU - Shi, Lijun
AU  - Shi L
AD  - Department of Orthopedics, Graduate School of Peking Union Medical College, 
      China-Japan Friendship Hospital, Beijing, 100029, P.R.China.
FAU - Mao, Tianli
AU  - Mao T
AD  - Department of Orthopedics, Peking University Third Hospital, Beijing, 100191, 
      P.R.China.
FAU - Luo, Pan
AU  - Luo P
AD  - Department of Orthopedics, Graduate School of Peking Union Medical College, 
      China-Japan Friendship Hospital, Beijing, 100029, P.R.China.
FAU - Li, Tengqi
AU  - Li T
AD  - Department of Orthopedics, Peking University China-Japan Friendship School of 
      Clinical Medicine, Beijing, 100029, P.R.China.
FAU - Gao, Fuqiang
AU  - Gao F
AD  - Center for Osteonecrosis and Joint Preserving & Reconstruction, China-Japan 
      Friendship Hospital, Beijing, 100029, P.R.China.
FAU - Sun, Wei
AU  - Sun W
AD  - Department of Orthopedics, Graduate School of Peking Union Medical College, 
      China-Japan Friendship Hospital, Beijing, 100029, P.R.China;Center for 
      Osteonecrosis and Joint Preserving & Reconstruction, China-Japan Friendship 
      Hospital, Beijing, 100029, P.R.China.
FAU - Li, Zirong
AU  - Li Z
AD  - Center for Osteonecrosis and Joint Preserving & Reconstruction, China-Japan 
      Friendship Hospital, Beijing, 100029, P.R.China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Flavonoids)
RN  - VNM47R2QSQ (icariin)
RN  - X4W7ZR7023 (Methylprednisolone)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - *Femur Head
MH  - Femur Head Necrosis
MH  - Flavonoids
MH  - Methylprednisolone
MH  - Rabbits
PMC - PMC8171965
OTO - NOTNLM
OT  - Micro-CT
OT  - Steroid-induced osteonecrosis of the femoral head
OT  - empty lacuna
OT  - icariin
OT  - rabbit
OT  - trabecula
COIS- 利益冲突：所有作者声明，在课题研究和文章撰写过程中不存在利益冲突。经费支持没有影响文章观点和对研究数据客观结果的统计分析及其报道。
EDAT- 2020/02/08 06:00
MHDA- 2020/02/29 06:00
PMCR- 2020/02/01
CRDT- 2020/02/08 06:00
PHST- 2020/02/08 06:00 [entrez]
PHST- 2020/02/08 06:00 [pubmed]
PHST- 2020/02/29 06:00 [medline]
PHST- 2020/02/01 00:00 [pmc-release]
AID - zgxfcjwkzz-34-2-206 [pii]
AID - 10.7507/1002-1892.201905112 [doi]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2020 Feb 15;34(2):206-212. doi: 
      10.7507/1002-1892.201905112.