PMID- 32032745
OWN - NLM
STAT- MEDLINE
DCOM- 20200406
LR  - 20210110
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 737
DP  - 2020 May 5
TI  - Functional expression of ZNF467 and PCBP2 supports adipogenic lineage commitment 
      in adipose-derived mesenchymal stem cells.
PG  - 144437
LID - S0378-1119(20)30106-2 [pii]
LID - 10.1016/j.gene.2020.144437 [doi]
AB  - Bone marrow-derived mesenchymal stromal/stem cells (BMSCs) have the potential to 
      be employed in many different skeletal therapies. A major limitation to utilizing 
      BMSCs as a therapeutic strategy in human disease and tissue regeneration is the 
      low cell numbers obtained from initial isolation necessitating multiple cell 
      passages that can lead to decreased cell quality. Adipose-derived mesenchymal 
      stromal/stem cells (AMSCs) have been proposed as an alternative cell source for 
      regenerative therapies; however the differentiation capacity of these cells 
      differs from BMSCs. To understand the differences between BMSCs and AMSCs, we 
      compared the global gene expression profiles of BMSCs and AMSCs and identified 
      two genes, PCBP2 and ZNF467 that were differentially expressed between AMSCs and 
      BMSCs. We demonstrate that PCBP2 and ZNF467 impact adipogenic but not osteogenic 
      differentiation, further supporting evidence that AMSCs and BMSCs appear to be 
      adapted to their microenvironment.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Gluscevic, Martina
AU  - Gluscevic M
AD  - Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, United States.
FAU - Paradise, Christopher R
AU  - Paradise CR
AD  - Mayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic, Rochester, MN, 
      United States; Center for Regenerative Medicine, Mayo Clinic, Rochester, MN, 
      United States.
FAU - Dudakovic, Amel
AU  - Dudakovic A
AD  - Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, United States; 
      Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, 
      United States.
FAU - Karperien, Marcel
AU  - Karperien M
AD  - Department of Developmental BioEngineering, University of Twente, Enschede, 
      Netherlands.
FAU - Dietz, Allan B
AU  - Dietz AB
AD  - Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 
      United States.
FAU - van Wijnen, Andre J
AU  - van Wijnen AJ
AD  - Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, United States; 
      Center for Regenerative Medicine, Mayo Clinic, Rochester, MN, United States; 
      Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, 
      United States. Electronic address: vanwijnen.andre@mayo.edu.
FAU - Deyle, David R
AU  - Deyle DR
AD  - Department of Orthopedic Surgery, Mayo Clinic, Rochester, MN, United States; 
      Department of Medical Genetics, Mayo Clinic, Rochester, MN, United States; 
      Department of Molecular Medicine, Mayo Clinic, Rochester, MN, United States. 
      Electronic address: deyle.david@mayo.edu.
LA  - eng
GR  - R01 AR067707/AR/NIAMS NIH HHS/United States
PT  - Journal Article
DEP - 20200204
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (PCBP2 protein, human)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Transcription Factors, General)
RN  - 0 (ZNF467 protein, human)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - *Cell Differentiation
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - RNA-Binding Proteins/genetics/*metabolism
MH  - Transcription Factors, General/genetics/*metabolism
OTO - NOTNLM
OT  - Adipogenic differentiation
OT  - Adipose-derived mesenchymal stromal/stem cells
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/02/08 06:00
MHDA- 2020/04/09 06:00
CRDT- 2020/02/08 06:00
PHST- 2020/01/27 00:00 [received]
PHST- 2020/02/03 00:00 [accepted]
PHST- 2020/02/08 06:00 [pubmed]
PHST- 2020/04/09 06:00 [medline]
PHST- 2020/02/08 06:00 [entrez]
AID - S0378-1119(20)30106-2 [pii]
AID - 10.1016/j.gene.2020.144437 [doi]
PST - ppublish
SO  - Gene. 2020 May 5;737:144437. doi: 10.1016/j.gene.2020.144437. Epub 2020 Feb 4.