PMID- 32032749
OWN - NLM
STAT- MEDLINE
DCOM- 20210419
LR  - 20210419
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 721
DP  - 2020 Mar 16
TI  - Heroin exposure and withdrawal differentially influence expression of NMDA 
      receptor NR2 subunits in the prelimbic region of rat medial prefrontal cortex.
PG  - 134819
LID - S0304-3940(20)30089-6 [pii]
LID - 10.1016/j.neulet.2020.134819 [doi]
AB  - It is widely reported that drug addiction involves the strengthening of specific 
      reward circuits through N-methyl-d-aspartic acid receptor (NMDAR)-dependent 
      synaptic potentiation, and several lines of evidence strongly implicate NMDA 
      receptor 2 (NR2) subunits in drug abuse. To explore the potential mechanism of 
      heroin dependence, this study examined changes in the expression levels of NR2 
      subunits NR2A-D in the prelimbic (PL) region of the medial prefrontal cortex 
      (mPFC) after repeated heroin administration and subsequent abstinence. The 
      conditioned place preference (CPP) test confirmed successful induction of heroin 
      dependence and withdrawal. Western blotting and qRT-PCR revealed no differences 
      in NR2A subunit expression among heroin-exposure, heroin-withdrawal, and control 
      group rats; in contrast, expression of NR2B was significantly higher in the 
      heroin-exposure group, whereas expression levels of NR2C and NR2D were 
      significantly higher in the heroin-withdrawal group relative to the controls. 
      Further studies are needed to identify the functional significance based on 
      alterations of NR2 subunits.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Zhu, Zaiman
AU  - Zhu Z
AD  - College of Life Sciences, Anhui Normal University, Wuhu, 241000, China; 
      Department of Physiology, Wannan Medical College, Wuhu, 241000, China.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Physiology, Wannan Medical College, Wuhu, 241000, China.
FAU - Jiang, Yuxin
AU  - Jiang Y
AD  - Department of Physiology, Wannan Medical College, Wuhu, 241000, China.
FAU - Hua, Tianmiao
AU  - Hua T
AD  - College of Life Sciences, Anhui Normal University, Wuhu, 241000, China. 
      Electronic address: tmhua@mail.ahnu.edu.cn.
FAU - Zhang, Changzheng
AU  - Zhang C
AD  - Department of Psychology & Guangdong Provincial Key Laboratory of Development and 
      Education for Special Needs Children, Lingnan Normal University, Zhanjiang, 
      524048, China. Electronic address: neurobiologyzhang@yahoo.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200204
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (NR2A NMDA receptor)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - 70D95007SX (Heroin)
SB  - IM
MH  - Animals
MH  - Gene Expression
MH  - Heroin/*adverse effects
MH  - Heroin Dependence/genetics/*metabolism
MH  - Male
MH  - Prefrontal Cortex/drug effects/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*biosynthesis/genetics
MH  - Substance Withdrawal Syndrome/genetics/*metabolism
OTO - NOTNLM
OT  - Heroin exposure
OT  - Heroin withdrawal
OT  - NMDA receptor
OT  - NR2 subunits
OT  - PL region
COIS- Declaration of Competing Interest The authors have no conflicts of interests.
EDAT- 2020/02/08 06:00
MHDA- 2021/04/20 06:00
CRDT- 2020/02/08 06:00
PHST- 2019/10/31 00:00 [received]
PHST- 2020/01/24 00:00 [revised]
PHST- 2020/02/03 00:00 [accepted]
PHST- 2020/02/08 06:00 [pubmed]
PHST- 2021/04/20 06:00 [medline]
PHST- 2020/02/08 06:00 [entrez]
AID - S0304-3940(20)30089-6 [pii]
AID - 10.1016/j.neulet.2020.134819 [doi]
PST - ppublish
SO  - Neurosci Lett. 2020 Mar 16;721:134819. doi: 10.1016/j.neulet.2020.134819. Epub 
      2020 Feb 4.