PMID- 32033425
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2076-0817 (Print)
IS  - 2076-0817 (Electronic)
IS  - 2076-0817 (Linking)
VI  - 9
IP  - 2
DP  - 2020 Feb 5
TI  - Comparison of the Transcriptome Response within the Swine Tracheobronchial 
      Lymphnode Following Infection with PRRSV, PCV-2 or IAV-S.
LID - 10.3390/pathogens9020099 [doi]
LID - 99
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) is a major 
      respiratory pathogen of swine that has become extremely costly to the swine 
      industry worldwide, often causing losses in production and animal life due to 
      their ease of spread. However, the intracellular changes that occur in pigs 
      following viral respiratory infections are still scantily understood for PRRSV, 
      as well as other viral respiratory infections. The aim of this study was to 
      acquire a better understanding of the PRRS disease by comparing gene expression 
      changes that occur in tracheobronchial lymph nodes (TBLN) of pigs infected with 
      either porcine reproductive and respiratory syndrome virus (PRRSV), porcine 
      circovirus type 2 (PCV-2), or swine influenza A virus (IAV-S) infections. The 
      study identified and compared gene expression changes in the TBLN of 80 pigs 
      following infection by PRRSV, PCV-2, IAV-S, or sham inoculation. Total RNA was 
      pooled for each group and time-point (1, 3, 6, and 14 dpi) to make 16 
      libraries-analyses are by Digital Gene Expression Tag Profiling (DGETP). The data 
      underwent standard filtering to generate a list of sequence tag raw counts that 
      were then analyzed using multidimensional and differential expression statistical 
      tests. The results showed that PRRSV, IAV-S and PCV-2 viral infections followed a 
      clinical course in the pigs typical of experimental infection of young pigs with 
      these viruses. Gene expression results echoed this course, as well as uncovered 
      genes related to intersecting and unique host immune responses to the three 
      viruses. By testing and observing the host response to other respiratory viruses, 
      our study has elucidated similarities and differences that can assist in the 
      development of vaccines and therapeutics that shorten or prevent a chronic PRRSV 
      infection.
FAU - Miller, Laura C
AU  - Miller LC
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA 50161, USA.
FAU - Fleming, Damarius S
AU  - Fleming DS
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA 50161, USA.
AD  - Oak Ridge Associated Universities/Oak Ridge Institute for Science and Education, 
      Oakridge, TN 37830, USA.
FAU - Lager, Kelly M
AU  - Lager KM
AD  - Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural 
      Research Service, Ames, IA 50161, USA.
LA  - eng
GR  - 08-247/National Pork Board/
GR  - DE-AC05-06OR23100/Oak Ridge Institute for Science and Education/
PT  - Journal Article
DEP - 20200205
PL  - Switzerland
TA  - Pathogens
JT  - Pathogens (Basel, Switzerland)
JID - 101596317
PMC - PMC7168592
OTO - NOTNLM
OT  - IAV-S
OT  - PCV-2
OT  - PRRSV
OT  - differential expression
OT  - immune response
OT  - lymph node
COIS- The authors declare no conflict of interest. Mention of trade names or commercial 
      products in this article is solely for the purpose of providing specific 
      information and does not imply recommendation or endorsement by the U.S. 
      Department of Agriculture. USDA is an equal opportunity provider and employer.
EDAT- 2020/02/09 06:00
MHDA- 2020/02/09 06:01
PMCR- 2020/02/01
CRDT- 2020/02/09 06:00
PHST- 2019/12/27 00:00 [received]
PHST- 2020/01/29 00:00 [revised]
PHST- 2020/02/04 00:00 [accepted]
PHST- 2020/02/09 06:00 [entrez]
PHST- 2020/02/09 06:00 [pubmed]
PHST- 2020/02/09 06:01 [medline]
PHST- 2020/02/01 00:00 [pmc-release]
AID - pathogens9020099 [pii]
AID - pathogens-09-00099 [pii]
AID - 10.3390/pathogens9020099 [doi]
PST - epublish
SO  - Pathogens. 2020 Feb 5;9(2):99. doi: 10.3390/pathogens9020099.