PMID- 32034794
OWN - NLM
STAT- MEDLINE
DCOM- 20210115
LR  - 20210115
IS  - 1439-0264 (Electronic)
IS  - 0340-2096 (Linking)
VI  - 49
IP  - 3
DP  - 2020 May
TI  - Differentiation of human umbilical cord mesenchymal stem cell into germ-like cell 
      under effect of co-culture with testicular cell tissue.
PG  - 359-364
LID - 10.1111/ahe.12537 [doi]
AB  - Supplements produced by mouse testicular cells (mTCs) and the interaction between 
      cells can increase the differentiation rate of human umbilical cord mesenchymal 
      stem cells (hUCMSCs) into the germ-like cells. We studied the differentiation 
      rate of hUCMSCs into the germ-like cells under effect of mTCs co-culturing. 
      Isolated hUCMSCs from postpartum human umbilical cords were cultured. Then, the 
      expression of mesenchymal (CD73, CD90 and CD105) and haematopoietic (CD34 and 
      CD45) markers of hUCMSCs were confirmed by flow cytometry. Then, the hUCMSCs were 
      cultured in four distinct groups: (a) control, (b) co-culture until D0, (c) 
      co-culture until D5 and (d) co-culture until D10, in order to differentiate into 
      the germ-like cells. After 10 days, the expression of OCT4, VASA, Fragilis and 
      SYCP3 genes were examined by Real-Time qPCR. The flow cytometry indicated a high 
      expression of mesenchymal markers and a low expression of haematopoietic markers 
      (CD73:98.6%, CD90: 99.1%, CD105: 99.5%, CD34: 4.22% and CD45: 2.54%). The 
      expression of OCT4 decreased during the time while the expression of VASA, 
      Fragilis and SYCP3 markers increased in the co-culture with testicular cells (p 
      value <.05). Co-culture with mTCs may be used as an effective method to 
      differentiate hUCMSCs into germ-like cells.
CI  - (c) 2020 Blackwell Verlag GmbH.
FAU - Majidi, Fatemeh
AU  - Majidi F
AD  - Department of Animal Physiology, Shahid Beheshti University, Tehran, Iran.
FAU - Bamehr, Hadi
AU  - Bamehr H
AD  - Research Center for Molecular Medicine, Hamadan University of Medical Sciences, 
      Hamadan, Iran.
FAU - Shalchian, Zohreh
AU  - Shalchian Z
AD  - Department of Food Chemistry, Science and Research Branch, Islamic Azad 
      University, Tehran, Iran.
FAU - Kouchakian, Mohammad-Reza
AU  - Kouchakian MR
AD  - Department of Anatomical Science, School of Medicine, Iran University of Medical 
      Science, Tehran, Iran.
FAU - Mohammadzadeh, Nima
AU  - Mohammadzadeh N
AD  - Department of Molecular Medicine, Cancer Biomedical Center, Tehran, Iran.
AD  - Department of Microbiology, School of Medicine, Iran University of Medical 
      Sciences, Tehran, Iran.
FAU - Khalili, Ahmad
AU  - Khalili A
AUID- ORCID: 0000-0002-5068-7889
AD  - Department of Molecular Medicine, Cancer Biomedical Center, Tehran, Iran.
AD  - Department of Immunology, School of Medical Sciences, Tarbiat Modares University, 
      Tehran, Iran.
LA  - eng
PT  - Journal Article
DEP - 20200207
PL  - Germany
TA  - Anat Histol Embryol
JT  - Anatomia, histologia, embryologia
JID - 7704218
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Humans
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology
MH  - Testis/cytology/metabolism
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - germ cell
OT  - mesenchymal stem cell
OT  - multipotent stem cells
OT  - testicular cells
EDAT- 2020/02/09 06:00
MHDA- 2021/01/16 06:00
CRDT- 2020/02/09 06:00
PHST- 2019/06/22 00:00 [received]
PHST- 2019/11/26 00:00 [revised]
PHST- 2020/01/12 00:00 [accepted]
PHST- 2020/02/09 06:00 [pubmed]
PHST- 2021/01/16 06:00 [medline]
PHST- 2020/02/09 06:00 [entrez]
AID - 10.1111/ahe.12537 [doi]
PST - ppublish
SO  - Anat Histol Embryol. 2020 May;49(3):359-364. doi: 10.1111/ahe.12537. Epub 2020 
      Feb 7.